Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700015 |
Resumo: | Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100%, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis. |
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Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniquesMycobacterium bovisdrug susceptibilityisoniaziderifoligotypingTransmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100%, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis.Instituto Oswaldo Cruz, Ministério da Saúde2004-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700015Memórias do Instituto Oswaldo Cruz v.99 n.7 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000700015info:eu-repo/semantics/openAccessParreiras,Patrícia MLobato,Francisco CFAlencar,Andréa PFigueiredo,Telma deGomes,Harrison MBoéchat,NeioLage,Andrey PAssis,Ronnie APereira,Márcia ASSouza,Patrícia RMota,Pedro MPCSuffys,Philip Neng2020-04-25T17:49:20Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:49.641Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
title |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
spellingShingle |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques Parreiras,Patrícia M Mycobacterium bovis drug susceptibility isoniazide rifoligotyping |
title_short |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
title_full |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
title_fullStr |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
title_full_unstemmed |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
title_sort |
Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques |
author |
Parreiras,Patrícia M |
author_facet |
Parreiras,Patrícia M Lobato,Francisco CF Alencar,Andréa P Figueiredo,Telma de Gomes,Harrison M Boéchat,Neio Lage,Andrey P Assis,Ronnie A Pereira,Márcia AS Souza,Patrícia R Mota,Pedro MPC Suffys,Philip N |
author_role |
author |
author2 |
Lobato,Francisco CF Alencar,Andréa P Figueiredo,Telma de Gomes,Harrison M Boéchat,Neio Lage,Andrey P Assis,Ronnie A Pereira,Márcia AS Souza,Patrícia R Mota,Pedro MPC Suffys,Philip N |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Parreiras,Patrícia M Lobato,Francisco CF Alencar,Andréa P Figueiredo,Telma de Gomes,Harrison M Boéchat,Neio Lage,Andrey P Assis,Ronnie A Pereira,Márcia AS Souza,Patrícia R Mota,Pedro MPC Suffys,Philip N |
dc.subject.por.fl_str_mv |
Mycobacterium bovis drug susceptibility isoniazide rifoligotyping |
topic |
Mycobacterium bovis drug susceptibility isoniazide rifoligotyping |
dc.description.none.fl_txt_mv |
Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100%, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis. |
description |
Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100%, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700015 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000700015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762004000700015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.99 n.7 2004 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937691799584768 |