Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias

Detalhes bibliográficos
Autor(a) principal: Thomas,A. W.
Data de Publicação: 1994
Outros Autores: Narum,D., Waters,A. P., Trape,J. F., Rogier,C., Gonçalves,A., Rosario,V., Druilhe,P., Mitchell,G. H., Dennis,D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600016
Resumo: The apical membrane antigen (AMA-1) family of malaria merozoite proteins is characterised by a high degree of inter-species conservation. Evidence that the protein (PK66/AMA-1) from the simian parasite Plasmodium knowlesi was protective in rhesus monkeys suggested that the 83kDa P. falciparum equivalent (PF83/AMA-1) should be investigated for protective effects in humans. Here we briefly review pertinent comparative data, and describe the use of an eukaryotic full length recombinant PF83/AMA-1 molecule to develop a sensitive ELISA for the determination of serological responses in endemic populations. The assay has revealed surprisingly high levels of humoral response to this quantitatively minor antigen. We also show that PK66/AMA-1 inhibitory mAb's are active against merozoites subsequent to release from schizont-infected red cells, further implicating AMA-1 molecules in red cell invasion.
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spelling Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malariasPlasmodium falciparumPlasmodium knowlesiiAMA-1PK66PF83merozoiteseroepidemiologyThe apical membrane antigen (AMA-1) family of malaria merozoite proteins is characterised by a high degree of inter-species conservation. Evidence that the protein (PK66/AMA-1) from the simian parasite Plasmodium knowlesi was protective in rhesus monkeys suggested that the 83kDa P. falciparum equivalent (PF83/AMA-1) should be investigated for protective effects in humans. Here we briefly review pertinent comparative data, and describe the use of an eukaryotic full length recombinant PF83/AMA-1 molecule to develop a sensitive ELISA for the determination of serological responses in endemic populations. The assay has revealed surprisingly high levels of humoral response to this quantitatively minor antigen. We also show that PK66/AMA-1 inhibitory mAb's are active against merozoites subsequent to release from schizont-infected red cells, further implicating AMA-1 molecules in red cell invasion.Instituto Oswaldo Cruz, Ministério da Saúde1994-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600016Memórias do Instituto Oswaldo Cruz v.89 suppl.2 1994reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761994000600016info:eu-repo/semantics/openAccessThomas,A. W.Narum,D.Waters,A. P.Trape,J. F.Rogier,C.Gonçalves,A.Rosario,V.Druilhe,P.Mitchell,G. H.Dennis,D.eng2020-04-25T17:47:19Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:18.414Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
title Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
spellingShingle Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
Thomas,A. W.
Plasmodium falciparum
Plasmodium knowlesii
AMA-1
PK66
PF83
merozoite
seroepidemiology
title_short Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
title_full Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
title_fullStr Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
title_full_unstemmed Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
title_sort Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
author Thomas,A. W.
author_facet Thomas,A. W.
Narum,D.
Waters,A. P.
Trape,J. F.
Rogier,C.
Gonçalves,A.
Rosario,V.
Druilhe,P.
Mitchell,G. H.
Dennis,D.
author_role author
author2 Narum,D.
Waters,A. P.
Trape,J. F.
Rogier,C.
Gonçalves,A.
Rosario,V.
Druilhe,P.
Mitchell,G. H.
Dennis,D.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Thomas,A. W.
Narum,D.
Waters,A. P.
Trape,J. F.
Rogier,C.
Gonçalves,A.
Rosario,V.
Druilhe,P.
Mitchell,G. H.
Dennis,D.
dc.subject.por.fl_str_mv Plasmodium falciparum
Plasmodium knowlesii
AMA-1
PK66
PF83
merozoite
seroepidemiology
topic Plasmodium falciparum
Plasmodium knowlesii
AMA-1
PK66
PF83
merozoite
seroepidemiology
dc.description.none.fl_txt_mv The apical membrane antigen (AMA-1) family of malaria merozoite proteins is characterised by a high degree of inter-species conservation. Evidence that the protein (PK66/AMA-1) from the simian parasite Plasmodium knowlesi was protective in rhesus monkeys suggested that the 83kDa P. falciparum equivalent (PF83/AMA-1) should be investigated for protective effects in humans. Here we briefly review pertinent comparative data, and describe the use of an eukaryotic full length recombinant PF83/AMA-1 molecule to develop a sensitive ELISA for the determination of serological responses in endemic populations. The assay has revealed surprisingly high levels of humoral response to this quantitatively minor antigen. We also show that PK66/AMA-1 inhibitory mAb's are active against merozoites subsequent to release from schizont-infected red cells, further implicating AMA-1 molecules in red cell invasion.
description The apical membrane antigen (AMA-1) family of malaria merozoite proteins is characterised by a high degree of inter-species conservation. Evidence that the protein (PK66/AMA-1) from the simian parasite Plasmodium knowlesi was protective in rhesus monkeys suggested that the 83kDa P. falciparum equivalent (PF83/AMA-1) should be investigated for protective effects in humans. Here we briefly review pertinent comparative data, and describe the use of an eukaryotic full length recombinant PF83/AMA-1 molecule to develop a sensitive ELISA for the determination of serological responses in endemic populations. The assay has revealed surprisingly high levels of humoral response to this quantitatively minor antigen. We also show that PK66/AMA-1 inhibitory mAb's are active against merozoites subsequent to release from schizont-infected red cells, further implicating AMA-1 molecules in red cell invasion.
publishDate 1994
dc.date.none.fl_str_mv 1994-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000600016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761994000600016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.89 suppl.2 1994
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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