Enterobius vermicularis: ancient DNA from north and south American human coprolites

Detalhes bibliográficos
Autor(a) principal: Iñiguez,Alena M
Data de Publicação: 2003
Outros Autores: Reinhard,Karl J, Araújo,Adauto, Ferreira,Luiz Fernando, Vicente,Ana Carolina P
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000900013
Resumo: A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.
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spelling Enterobius vermicularis: ancient DNA from north and south American human coprolitesancient DNAEnterobius vermiculariscoprolitesA molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.Instituto Oswaldo Cruz, Ministério da Saúde2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000900013Memórias do Instituto Oswaldo Cruz v.98 suppl.1 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000900013info:eu-repo/semantics/openAccessIñiguez,Alena MReinhard,Karl JAraújo,AdautoFerreira,Luiz FernandoVicente,Ana Carolina Peng2020-04-25T17:49:11Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:21.704Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Enterobius vermicularis: ancient DNA from north and south American human coprolites
title Enterobius vermicularis: ancient DNA from north and south American human coprolites
spellingShingle Enterobius vermicularis: ancient DNA from north and south American human coprolites
Iñiguez,Alena M
ancient DNA
Enterobius vermicularis
coprolites
title_short Enterobius vermicularis: ancient DNA from north and south American human coprolites
title_full Enterobius vermicularis: ancient DNA from north and south American human coprolites
title_fullStr Enterobius vermicularis: ancient DNA from north and south American human coprolites
title_full_unstemmed Enterobius vermicularis: ancient DNA from north and south American human coprolites
title_sort Enterobius vermicularis: ancient DNA from north and south American human coprolites
author Iñiguez,Alena M
author_facet Iñiguez,Alena M
Reinhard,Karl J
Araújo,Adauto
Ferreira,Luiz Fernando
Vicente,Ana Carolina P
author_role author
author2 Reinhard,Karl J
Araújo,Adauto
Ferreira,Luiz Fernando
Vicente,Ana Carolina P
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Iñiguez,Alena M
Reinhard,Karl J
Araújo,Adauto
Ferreira,Luiz Fernando
Vicente,Ana Carolina P
dc.subject.por.fl_str_mv ancient DNA
Enterobius vermicularis
coprolites
topic ancient DNA
Enterobius vermicularis
coprolites
dc.description.none.fl_txt_mv A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.
description A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000900013
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000900013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762003000900013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.98 suppl.1 2003
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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