In vitro activity of amphotericin B cochleates against Leishmania chagasi

Detalhes bibliográficos
Autor(a) principal: Sesana,Aretha Molina
Data de Publicação: 2011
Outros Autores: Monti-Rocha,Renata, Vinhas,Solange Alves, Morais,Carlos Gustavo, Dietze,Reynaldo, Lemos,Elenice Moreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200022
Resumo: Cochleate delivery vehicles are a novel lipid-based system with potential for delivery of amphotericin B (AmB). In this study, the efficacy of cochleates was evaluated by examining the in vitro activity of AmB cochleates (CAMB) against Leishmania chagasi in a macrophage model of infection. We demonstrate that CAMB is nontoxic to macrophages at concentrations as high as 2.5 μg/mL, whereas the conventional formulation, AmB deoxycholate, showed high toxicity at this concentration. The in vitro activity of CAMB against L. chagasi was found to be similar to that of the reference drug AmB deoxycholate, with ED50s of 0.017 μg/mL and 0.021 μg/mL, respectively. Considering that L. chagasi affects organs amenable to cochleate-mediated delivery of AmB, we hypothesize that CAMB will be an effective lipid system for the treatment of visceral leishmaniasis.
id FIOCRUZ-4_ac1ff79e40eeb812756d00f9aa9b8393
oai_identifier_str oai:scielo:S0074-02762011000200022
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling In vitro activity of amphotericin B cochleates against Leishmania chagasiamphotericin B cochleatesLeishmania chagasiin vitro activityCochleate delivery vehicles are a novel lipid-based system with potential for delivery of amphotericin B (AmB). In this study, the efficacy of cochleates was evaluated by examining the in vitro activity of AmB cochleates (CAMB) against Leishmania chagasi in a macrophage model of infection. We demonstrate that CAMB is nontoxic to macrophages at concentrations as high as 2.5 μg/mL, whereas the conventional formulation, AmB deoxycholate, showed high toxicity at this concentration. The in vitro activity of CAMB against L. chagasi was found to be similar to that of the reference drug AmB deoxycholate, with ED50s of 0.017 μg/mL and 0.021 μg/mL, respectively. Considering that L. chagasi affects organs amenable to cochleate-mediated delivery of AmB, we hypothesize that CAMB will be an effective lipid system for the treatment of visceral leishmaniasis.Instituto Oswaldo Cruz, Ministério da Saúde2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200022Memórias do Instituto Oswaldo Cruz v.106 n.2 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000200022info:eu-repo/semantics/openAccessSesana,Aretha MolinaMonti-Rocha,RenataVinhas,Solange AlvesMorais,Carlos GustavoDietze,ReynaldoLemos,Elenice Moreiraeng2020-04-25T17:50:59Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:33.159Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv In vitro activity of amphotericin B cochleates against Leishmania chagasi
title In vitro activity of amphotericin B cochleates against Leishmania chagasi
spellingShingle In vitro activity of amphotericin B cochleates against Leishmania chagasi
Sesana,Aretha Molina
amphotericin B cochleates
Leishmania chagasi
in vitro activity
title_short In vitro activity of amphotericin B cochleates against Leishmania chagasi
title_full In vitro activity of amphotericin B cochleates against Leishmania chagasi
title_fullStr In vitro activity of amphotericin B cochleates against Leishmania chagasi
title_full_unstemmed In vitro activity of amphotericin B cochleates against Leishmania chagasi
title_sort In vitro activity of amphotericin B cochleates against Leishmania chagasi
author Sesana,Aretha Molina
author_facet Sesana,Aretha Molina
Monti-Rocha,Renata
Vinhas,Solange Alves
Morais,Carlos Gustavo
Dietze,Reynaldo
Lemos,Elenice Moreira
author_role author
author2 Monti-Rocha,Renata
Vinhas,Solange Alves
Morais,Carlos Gustavo
Dietze,Reynaldo
Lemos,Elenice Moreira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Sesana,Aretha Molina
Monti-Rocha,Renata
Vinhas,Solange Alves
Morais,Carlos Gustavo
Dietze,Reynaldo
Lemos,Elenice Moreira
dc.subject.por.fl_str_mv amphotericin B cochleates
Leishmania chagasi
in vitro activity
topic amphotericin B cochleates
Leishmania chagasi
in vitro activity
dc.description.none.fl_txt_mv Cochleate delivery vehicles are a novel lipid-based system with potential for delivery of amphotericin B (AmB). In this study, the efficacy of cochleates was evaluated by examining the in vitro activity of AmB cochleates (CAMB) against Leishmania chagasi in a macrophage model of infection. We demonstrate that CAMB is nontoxic to macrophages at concentrations as high as 2.5 μg/mL, whereas the conventional formulation, AmB deoxycholate, showed high toxicity at this concentration. The in vitro activity of CAMB against L. chagasi was found to be similar to that of the reference drug AmB deoxycholate, with ED50s of 0.017 μg/mL and 0.021 μg/mL, respectively. Considering that L. chagasi affects organs amenable to cochleate-mediated delivery of AmB, we hypothesize that CAMB will be an effective lipid system for the treatment of visceral leishmaniasis.
description Cochleate delivery vehicles are a novel lipid-based system with potential for delivery of amphotericin B (AmB). In this study, the efficacy of cochleates was evaluated by examining the in vitro activity of AmB cochleates (CAMB) against Leishmania chagasi in a macrophage model of infection. We demonstrate that CAMB is nontoxic to macrophages at concentrations as high as 2.5 μg/mL, whereas the conventional formulation, AmB deoxycholate, showed high toxicity at this concentration. The in vitro activity of CAMB against L. chagasi was found to be similar to that of the reference drug AmB deoxycholate, with ED50s of 0.017 μg/mL and 0.021 μg/mL, respectively. Considering that L. chagasi affects organs amenable to cochleate-mediated delivery of AmB, we hypothesize that CAMB will be an effective lipid system for the treatment of visceral leishmaniasis.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200022
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000200022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.2 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937708929122304

Registros relacionados