Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs

Detalhes bibliográficos
Autor(a) principal: Semião-Santos,SJ
Data de Publicação: 1996
Outros Autores: Abranches,P, Silva-Pereira,MCD, Santos-Gomes,GM, Fernandes,JP, Vetter,JCM
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600018
Resumo: A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude.
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spelling Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirscaninevulpineleishmaniasisdirect agglutination testimmunofluorescence techniqueA direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude.Instituto Oswaldo Cruz, Ministério da Saúde1996-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600018Memórias do Instituto Oswaldo Cruz v.91 n.6 1996reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761996000600018info:eu-repo/semantics/openAccessSemião-Santos,SJAbranches,PSilva-Pereira,MCDSantos-Gomes,GMFernandes,JPVetter,JCMeng2020-04-25T17:47:36Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:14.388Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
title Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
spellingShingle Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
Semião-Santos,SJ
canine
vulpine
leishmaniasis
direct agglutination test
immunofluorescence technique
title_short Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
title_full Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
title_fullStr Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
title_full_unstemmed Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
title_sort Reliability of serological methods for detection of leishmaniasis in portuguese domestic and wild reservoirs
author Semião-Santos,SJ
author_facet Semião-Santos,SJ
Abranches,P
Silva-Pereira,MCD
Santos-Gomes,GM
Fernandes,JP
Vetter,JCM
author_role author
author2 Abranches,P
Silva-Pereira,MCD
Santos-Gomes,GM
Fernandes,JP
Vetter,JCM
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Semião-Santos,SJ
Abranches,P
Silva-Pereira,MCD
Santos-Gomes,GM
Fernandes,JP
Vetter,JCM
dc.subject.por.fl_str_mv canine
vulpine
leishmaniasis
direct agglutination test
immunofluorescence technique
topic canine
vulpine
leishmaniasis
direct agglutination test
immunofluorescence technique
dc.description.none.fl_txt_mv A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude.
description A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres >1:320 and >1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence of L. infantum infection in the canine and vulpine populations appear to be of high magnitude.
publishDate 1996
dc.date.none.fl_str_mv 1996-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761996000600018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.91 n.6 1996
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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