Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/116819 |
Resumo: | A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude. |
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Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirsCanineDirect agglutination testImmunofluorescence techniqueLeishmaniasisVulpineParasitologia MédicaClinical BiochemistryImmunologyVirologyInfectious DiseasesMicrobiology (medical)SDG 3 - Good Health and Well-beingA direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude.Instituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)RUNSemião-Santos, S. J.Abranches, PSilva-Pereira, M. C DSantos-Gomes, G. M.Fernandes, J. P.Vetter, J. C M2021-05-03T22:31:49Z1996-111996-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttp://hdl.handle.net/10362/116819eng0074-0276PURE: 2397691https://doi.org/10.1590/s0074-02761996000600018info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:35Zoai:run.unl.pt:10362/116819Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:18.127379Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
title |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
spellingShingle |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs Semião-Santos, S. J. Canine Direct agglutination test Immunofluorescence technique Leishmaniasis Vulpine Parasitologia Médica Clinical Biochemistry Immunology Virology Infectious Diseases Microbiology (medical) SDG 3 - Good Health and Well-being |
title_short |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
title_full |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
title_fullStr |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
title_full_unstemmed |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
title_sort |
Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs |
author |
Semião-Santos, S. J. |
author_facet |
Semião-Santos, S. J. Abranches, P Silva-Pereira, M. C D Santos-Gomes, G. M. Fernandes, J. P. Vetter, J. C M |
author_role |
author |
author2 |
Abranches, P Silva-Pereira, M. C D Santos-Gomes, G. M. Fernandes, J. P. Vetter, J. C M |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) RUN |
dc.contributor.author.fl_str_mv |
Semião-Santos, S. J. Abranches, P Silva-Pereira, M. C D Santos-Gomes, G. M. Fernandes, J. P. Vetter, J. C M |
dc.subject.por.fl_str_mv |
Canine Direct agglutination test Immunofluorescence technique Leishmaniasis Vulpine Parasitologia Médica Clinical Biochemistry Immunology Virology Infectious Diseases Microbiology (medical) SDG 3 - Good Health and Well-being |
topic |
Canine Direct agglutination test Immunofluorescence technique Leishmaniasis Vulpine Parasitologia Médica Clinical Biochemistry Immunology Virology Infectious Diseases Microbiology (medical) SDG 3 - Good Health and Well-being |
description |
A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-11 1996-11-01T00:00:00Z 2021-05-03T22:31:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/116819 |
url |
http://hdl.handle.net/10362/116819 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0074-0276 PURE: 2397691 https://doi.org/10.1590/s0074-02761996000600018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138042688045056 |