Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs

Detalhes bibliográficos
Autor(a) principal: Semião-Santos, S. J.
Data de Publicação: 1996
Outros Autores: Abranches, P, Silva-Pereira, M. C D, Santos-Gomes, G. M., Fernandes, J. P., Vetter, J. C M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/116819
Resumo: A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude.
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spelling Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirsCanineDirect agglutination testImmunofluorescence techniqueLeishmaniasisVulpineParasitologia MédicaClinical BiochemistryImmunologyVirologyInfectious DiseasesMicrobiology (medical)SDG 3 - Good Health and Well-beingA direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude.Instituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)RUNSemião-Santos, S. J.Abranches, PSilva-Pereira, M. C DSantos-Gomes, G. M.Fernandes, J. P.Vetter, J. C M2021-05-03T22:31:49Z1996-111996-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttp://hdl.handle.net/10362/116819eng0074-0276PURE: 2397691https://doi.org/10.1590/s0074-02761996000600018info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:59:35Zoai:run.unl.pt:10362/116819Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:18.127379Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
title Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
spellingShingle Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
Semião-Santos, S. J.
Canine
Direct agglutination test
Immunofluorescence technique
Leishmaniasis
Vulpine
Parasitologia Médica
Clinical Biochemistry
Immunology
Virology
Infectious Diseases
Microbiology (medical)
SDG 3 - Good Health and Well-being
title_short Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
title_full Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
title_fullStr Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
title_full_unstemmed Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
title_sort Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs
author Semião-Santos, S. J.
author_facet Semião-Santos, S. J.
Abranches, P
Silva-Pereira, M. C D
Santos-Gomes, G. M.
Fernandes, J. P.
Vetter, J. C M
author_role author
author2 Abranches, P
Silva-Pereira, M. C D
Santos-Gomes, G. M.
Fernandes, J. P.
Vetter, J. C M
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Higiene e Medicina Tropical (IHMT)
Centro de Malária e outras Doenças Tropicais (CMDT)
RUN
dc.contributor.author.fl_str_mv Semião-Santos, S. J.
Abranches, P
Silva-Pereira, M. C D
Santos-Gomes, G. M.
Fernandes, J. P.
Vetter, J. C M
dc.subject.por.fl_str_mv Canine
Direct agglutination test
Immunofluorescence technique
Leishmaniasis
Vulpine
Parasitologia Médica
Clinical Biochemistry
Immunology
Virology
Infectious Diseases
Microbiology (medical)
SDG 3 - Good Health and Well-being
topic Canine
Direct agglutination test
Immunofluorescence technique
Leishmaniasis
Vulpine
Parasitologia Médica
Clinical Biochemistry
Immunology
Virology
Infectious Diseases
Microbiology (medical)
SDG 3 - Good Health and Well-being
description A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude.
publishDate 1996
dc.date.none.fl_str_mv 1996-11
1996-11-01T00:00:00Z
2021-05-03T22:31:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/116819
url http://hdl.handle.net/10362/116819
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0074-0276
PURE: 2397691
https://doi.org/10.1590/s0074-02761996000600018
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eu_rights_str_mv openAccess
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