An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels

Detalhes bibliográficos
Autor(a) principal: Gonçalves,Antonio M.
Data de Publicação: 1990
Outros Autores: Nehme,Nedia S., Morel,Carlos M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017
Resumo: A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
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spelling An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gelssilver stainingschizodeme analysisDNARNAproteintrypanosomatidsA simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.Instituto Oswaldo Cruz, Ministério da Saúde1990-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017Memórias do Instituto Oswaldo Cruz v.85 n.1 1990reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761990000100017info:eu-repo/semantics/openAccessGonçalves,Antonio M.Nehme,Nedia S.Morel,Carlos M.eng2020-04-25T17:46:28Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:03:39.895Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
spellingShingle An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
Gonçalves,Antonio M.
silver staining
schizodeme analysis
DNA
RNA
protein
trypanosomatids
title_short An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_full An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_fullStr An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_full_unstemmed An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
title_sort An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
author Gonçalves,Antonio M.
author_facet Gonçalves,Antonio M.
Nehme,Nedia S.
Morel,Carlos M.
author_role author
author2 Nehme,Nedia S.
Morel,Carlos M.
author2_role author
author
dc.contributor.author.fl_str_mv Gonçalves,Antonio M.
Nehme,Nedia S.
Morel,Carlos M.
dc.subject.por.fl_str_mv silver staining
schizodeme analysis
DNA
RNA
protein
trypanosomatids
topic silver staining
schizodeme analysis
DNA
RNA
protein
trypanosomatids
dc.description.none.fl_txt_mv A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
description A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
publishDate 1990
dc.date.none.fl_str_mv 1990-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761990000100017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.85 n.1 1990
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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