A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Detalhes bibliográficos
Autor(a) principal: Qiu,Shanlian
Data de Publicação: 2012
Outros Autores: Chen,Jichen, Lin,Si, Lin,Xinjian
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029
Resumo: Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
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network_name_str Brazilian Journal of Microbiology
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spelling A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gelBacked gelDNASilver stainingEight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.Sociedade Brasileira de Microbiologia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029Brazilian Journal of Microbiology v.43 n.2 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000200029info:eu-repo/semantics/openAccessQiu,ShanlianChen,JichenLin,SiLin,Xinjianeng2012-08-07T00:00:00Zoai:scielo:S1517-83822012000200029Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-08-07T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
spellingShingle A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
Qiu,Shanlian
Backed gel
DNA
Silver staining
title_short A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_full A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_fullStr A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_full_unstemmed A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_sort A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
author Qiu,Shanlian
author_facet Qiu,Shanlian
Chen,Jichen
Lin,Si
Lin,Xinjian
author_role author
author2 Chen,Jichen
Lin,Si
Lin,Xinjian
author2_role author
author
author
dc.contributor.author.fl_str_mv Qiu,Shanlian
Chen,Jichen
Lin,Si
Lin,Xinjian
dc.subject.por.fl_str_mv Backed gel
DNA
Silver staining
topic Backed gel
DNA
Silver staining
description Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822012000200029
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.43 n.2 2012
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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