Molecular and biological diversity of HIV-1 in Brazil

Detalhes bibliográficos
Autor(a) principal: Couto-Fernandez,José Carlos
Data de Publicação: 1992
Outros Autores: Morgado,Mariza Gonçalves, Santos,Jairo Ivo dos, Galvão-Castro,Bernardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200013
Resumo: To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.
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spelling Molecular and biological diversity of HIV-1 in BrazilBrazilian HIVvirulencerestriction analysisviral diversityTo determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.Instituto Oswaldo Cruz, Ministério da Saúde1992-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200013Memórias do Instituto Oswaldo Cruz v.87 n.2 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000200013info:eu-repo/semantics/openAccessCouto-Fernandez,José CarlosMorgado,Mariza GonçalvesSantos,Jairo Ivo dosGalvão-Castro,Bernardoeng2020-04-25T17:46:45Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:36.734Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular and biological diversity of HIV-1 in Brazil
title Molecular and biological diversity of HIV-1 in Brazil
spellingShingle Molecular and biological diversity of HIV-1 in Brazil
Couto-Fernandez,José Carlos
Brazilian HIV
virulence
restriction analysis
viral diversity
title_short Molecular and biological diversity of HIV-1 in Brazil
title_full Molecular and biological diversity of HIV-1 in Brazil
title_fullStr Molecular and biological diversity of HIV-1 in Brazil
title_full_unstemmed Molecular and biological diversity of HIV-1 in Brazil
title_sort Molecular and biological diversity of HIV-1 in Brazil
author Couto-Fernandez,José Carlos
author_facet Couto-Fernandez,José Carlos
Morgado,Mariza Gonçalves
Santos,Jairo Ivo dos
Galvão-Castro,Bernardo
author_role author
author2 Morgado,Mariza Gonçalves
Santos,Jairo Ivo dos
Galvão-Castro,Bernardo
author2_role author
author
author
dc.contributor.author.fl_str_mv Couto-Fernandez,José Carlos
Morgado,Mariza Gonçalves
Santos,Jairo Ivo dos
Galvão-Castro,Bernardo
dc.subject.por.fl_str_mv Brazilian HIV
virulence
restriction analysis
viral diversity
topic Brazilian HIV
virulence
restriction analysis
viral diversity
dc.description.none.fl_txt_mv To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.
description To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.
publishDate 1992
dc.date.none.fl_str_mv 1992-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200013
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000200013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 n.2 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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