Environmental influences on antibody-enhanced dengue disease outcomes
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800010 |
Resumo: | Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE. |
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Environmental influences on antibody-enhanced dengue disease outcomesexperimental dengue infectionenriched environmentantibody-enhanced dengue diseaseserial dengue infectionT-lymphocytesBecause an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE.Instituto Oswaldo Cruz, Ministério da Saúde2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800010Memórias do Instituto Oswaldo Cruz v.107 n.8 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000800010info:eu-repo/semantics/openAccessDiniz,Daniel GuerreiroFôro,César Augusto RaiolTuriel,Maíra C PereiraSosthenes,Marcia CKDemachki,SâmiaGomes,Giovanni FreitasRego,Carla M DamascenoMagalhães,Marina CutrimPinho,Brunno GomesRamos,Juliana PastanaCasseb,Samir M MoraesBrito,Maysa de VasconcelosSilva,Eliana Vieira Pinto daNunes,Marcio Roberto TeixeiraDiniz,José Antonio PicançoCunningham,ColmPerry,Victor HughVasconcelos,Pedro F CostaDiniz,Cristovam W Picançoeng2020-04-25T17:51:18Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:38.655Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Environmental influences on antibody-enhanced dengue disease outcomes |
title |
Environmental influences on antibody-enhanced dengue disease outcomes |
spellingShingle |
Environmental influences on antibody-enhanced dengue disease outcomes Diniz,Daniel Guerreiro experimental dengue infection enriched environment antibody-enhanced dengue disease serial dengue infection T-lymphocytes |
title_short |
Environmental influences on antibody-enhanced dengue disease outcomes |
title_full |
Environmental influences on antibody-enhanced dengue disease outcomes |
title_fullStr |
Environmental influences on antibody-enhanced dengue disease outcomes |
title_full_unstemmed |
Environmental influences on antibody-enhanced dengue disease outcomes |
title_sort |
Environmental influences on antibody-enhanced dengue disease outcomes |
author |
Diniz,Daniel Guerreiro |
author_facet |
Diniz,Daniel Guerreiro Fôro,César Augusto Raiol Turiel,Maíra C Pereira Sosthenes,Marcia CK Demachki,Sâmia Gomes,Giovanni Freitas Rego,Carla M Damasceno Magalhães,Marina Cutrim Pinho,Brunno Gomes Ramos,Juliana Pastana Casseb,Samir M Moraes Brito,Maysa de Vasconcelos Silva,Eliana Vieira Pinto da Nunes,Marcio Roberto Teixeira Diniz,José Antonio Picanço Cunningham,Colm Perry,Victor Hugh Vasconcelos,Pedro F Costa Diniz,Cristovam W Picanço |
author_role |
author |
author2 |
Fôro,César Augusto Raiol Turiel,Maíra C Pereira Sosthenes,Marcia CK Demachki,Sâmia Gomes,Giovanni Freitas Rego,Carla M Damasceno Magalhães,Marina Cutrim Pinho,Brunno Gomes Ramos,Juliana Pastana Casseb,Samir M Moraes Brito,Maysa de Vasconcelos Silva,Eliana Vieira Pinto da Nunes,Marcio Roberto Teixeira Diniz,José Antonio Picanço Cunningham,Colm Perry,Victor Hugh Vasconcelos,Pedro F Costa Diniz,Cristovam W Picanço |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Diniz,Daniel Guerreiro Fôro,César Augusto Raiol Turiel,Maíra C Pereira Sosthenes,Marcia CK Demachki,Sâmia Gomes,Giovanni Freitas Rego,Carla M Damasceno Magalhães,Marina Cutrim Pinho,Brunno Gomes Ramos,Juliana Pastana Casseb,Samir M Moraes Brito,Maysa de Vasconcelos Silva,Eliana Vieira Pinto da Nunes,Marcio Roberto Teixeira Diniz,José Antonio Picanço Cunningham,Colm Perry,Victor Hugh Vasconcelos,Pedro F Costa Diniz,Cristovam W Picanço |
dc.subject.por.fl_str_mv |
experimental dengue infection enriched environment antibody-enhanced dengue disease serial dengue infection T-lymphocytes |
topic |
experimental dengue infection enriched environment antibody-enhanced dengue disease serial dengue infection T-lymphocytes |
dc.description.none.fl_txt_mv |
Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE. |
description |
Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800010 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762012000800010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.107 n.8 2012 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937712966139904 |