A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100350 |
Resumo: | BACKGROUND Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. METHODS AND FINDINGS Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. CONCLUSIONS These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient. |
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A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analysesSporothrixgenotypic analysesPCR fingerprintexperimental murine modelvirulence BACKGROUND Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. METHODS AND FINDINGS Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. CONCLUSIONS These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient.Instituto Oswaldo Cruz, Ministério da Saúde2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100350Memórias do Instituto Oswaldo Cruz v.114 2019reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760190260info:eu-repo/semantics/openAccessOliveira,Manoel Marques EAlmeida-Paes,RodrigoCorrêa-Moreira,DaniellyBorba,Cintia de MoraesMenezes,Rodrigo CaldasFreitas,Dayvison Francis Saraivado Valle,Antonio Carlos FrancesconiSchubach,Armando de OliveiraBarros,Monica Bastos de LimaNosanchuk,Joshua DGutierrez-Galhardo,Maria ClaraZancopé-Oliveira,Rosely Mariaeng2020-04-25T17:53:00Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:22:38.878Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
title |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
spellingShingle |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses Oliveira,Manoel Marques E Sporothrix genotypic analyses PCR fingerprint experimental murine model virulence |
title_short |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
title_full |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
title_fullStr |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
title_full_unstemmed |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
title_sort |
A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses |
author |
Oliveira,Manoel Marques E |
author_facet |
Oliveira,Manoel Marques E Almeida-Paes,Rodrigo Corrêa-Moreira,Danielly Borba,Cintia de Moraes Menezes,Rodrigo Caldas Freitas,Dayvison Francis Saraiva do Valle,Antonio Carlos Francesconi Schubach,Armando de Oliveira Barros,Monica Bastos de Lima Nosanchuk,Joshua D Gutierrez-Galhardo,Maria Clara Zancopé-Oliveira,Rosely Maria |
author_role |
author |
author2 |
Almeida-Paes,Rodrigo Corrêa-Moreira,Danielly Borba,Cintia de Moraes Menezes,Rodrigo Caldas Freitas,Dayvison Francis Saraiva do Valle,Antonio Carlos Francesconi Schubach,Armando de Oliveira Barros,Monica Bastos de Lima Nosanchuk,Joshua D Gutierrez-Galhardo,Maria Clara Zancopé-Oliveira,Rosely Maria |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Manoel Marques E Almeida-Paes,Rodrigo Corrêa-Moreira,Danielly Borba,Cintia de Moraes Menezes,Rodrigo Caldas Freitas,Dayvison Francis Saraiva do Valle,Antonio Carlos Francesconi Schubach,Armando de Oliveira Barros,Monica Bastos de Lima Nosanchuk,Joshua D Gutierrez-Galhardo,Maria Clara Zancopé-Oliveira,Rosely Maria |
dc.subject.por.fl_str_mv |
Sporothrix genotypic analyses PCR fingerprint experimental murine model virulence |
topic |
Sporothrix genotypic analyses PCR fingerprint experimental murine model virulence |
dc.description.none.fl_txt_mv |
BACKGROUND Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. METHODS AND FINDINGS Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. CONCLUSIONS These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient. |
description |
BACKGROUND Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. METHODS AND FINDINGS Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. CONCLUSIONS These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100350 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100350 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760190260 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.114 2019 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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