Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania

Detalhes bibliográficos
Autor(a) principal: Guimarães,Tânia Mara Pinto Dabés
Data de Publicação: 1996
Outros Autores: Toledo,Vicente de Paulo Coelho Peixoto de, Costa,Carlos Alberto da, Costa,Roberto Teodoro da, Genaro,Odair, Williams,Paul, Mayrink,Wilson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000100010
Resumo: A comparative study was undertaken on the immunogenic properties of 63kDa glycoproteins obtained from five different strains/species of Leishmania and assessed in C57BL/10 mice. The humoral immune response was assessed by ELISA against the five different antigens of the immunized animals. The cellular immune response was derived from Leishmania. The response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. The presence of ³-IFN and IL-2, and the absence of IL-4 in the supernatants of cells stimulated by L. amazonensis antigen established that the cellular response is of Th1 type. The five glycoproteins tested were equally effective in protecting C57BL/10 mice against challenge by L. amazonensis. About 50% of the immunized animals were protected for six months.
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spelling Assessment of immunity induced in mice by glycoproteins derived from different strains and species of LeishmaniaLeishmaniaglycoproteinsmonoclonal antibodiesA comparative study was undertaken on the immunogenic properties of 63kDa glycoproteins obtained from five different strains/species of Leishmania and assessed in C57BL/10 mice. The humoral immune response was assessed by ELISA against the five different antigens of the immunized animals. The cellular immune response was derived from Leishmania. The response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. The presence of ³-IFN and IL-2, and the absence of IL-4 in the supernatants of cells stimulated by L. amazonensis antigen established that the cellular response is of Th1 type. The five glycoproteins tested were equally effective in protecting C57BL/10 mice against challenge by L. amazonensis. About 50% of the immunized animals were protected for six months.Instituto Oswaldo Cruz, Ministério da Saúde1996-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000100010Memórias do Instituto Oswaldo Cruz v.91 n.1 1996reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761996000100010info:eu-repo/semantics/openAccessGuimarães,Tânia Mara Pinto DabésToledo,Vicente de Paulo Coelho Peixoto deCosta,Carlos Alberto daCosta,Roberto Teodoro daGenaro,OdairWilliams,PaulMayrink,Wilsoneng2020-04-25T17:47:30Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:50.199Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
title Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
spellingShingle Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
Guimarães,Tânia Mara Pinto Dabés
Leishmania
glycoproteins
monoclonal antibodies
title_short Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
title_full Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
title_fullStr Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
title_full_unstemmed Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
title_sort Assessment of immunity induced in mice by glycoproteins derived from different strains and species of Leishmania
author Guimarães,Tânia Mara Pinto Dabés
author_facet Guimarães,Tânia Mara Pinto Dabés
Toledo,Vicente de Paulo Coelho Peixoto de
Costa,Carlos Alberto da
Costa,Roberto Teodoro da
Genaro,Odair
Williams,Paul
Mayrink,Wilson
author_role author
author2 Toledo,Vicente de Paulo Coelho Peixoto de
Costa,Carlos Alberto da
Costa,Roberto Teodoro da
Genaro,Odair
Williams,Paul
Mayrink,Wilson
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães,Tânia Mara Pinto Dabés
Toledo,Vicente de Paulo Coelho Peixoto de
Costa,Carlos Alberto da
Costa,Roberto Teodoro da
Genaro,Odair
Williams,Paul
Mayrink,Wilson
dc.subject.por.fl_str_mv Leishmania
glycoproteins
monoclonal antibodies
topic Leishmania
glycoproteins
monoclonal antibodies
dc.description.none.fl_txt_mv A comparative study was undertaken on the immunogenic properties of 63kDa glycoproteins obtained from five different strains/species of Leishmania and assessed in C57BL/10 mice. The humoral immune response was assessed by ELISA against the five different antigens of the immunized animals. The cellular immune response was derived from Leishmania. The response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. The presence of ³-IFN and IL-2, and the absence of IL-4 in the supernatants of cells stimulated by L. amazonensis antigen established that the cellular response is of Th1 type. The five glycoproteins tested were equally effective in protecting C57BL/10 mice against challenge by L. amazonensis. About 50% of the immunized animals were protected for six months.
description A comparative study was undertaken on the immunogenic properties of 63kDa glycoproteins obtained from five different strains/species of Leishmania and assessed in C57BL/10 mice. The humoral immune response was assessed by ELISA against the five different antigens of the immunized animals. The cellular immune response was derived from Leishmania. The response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. The presence of ³-IFN and IL-2, and the absence of IL-4 in the supernatants of cells stimulated by L. amazonensis antigen established that the cellular response is of Th1 type. The five glycoproteins tested were equally effective in protecting C57BL/10 mice against challenge by L. amazonensis. About 50% of the immunized animals were protected for six months.
publishDate 1996
dc.date.none.fl_str_mv 1996-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000100010
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761996000100010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.91 n.1 1996
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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