CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1995 |
| Outros Autores: | |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Memórias do Instituto Oswaldo Cruz |
| Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000100012 |
Resumo: | We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania. |
| id |
FIOCRUZ-4_bb2b4448404a9192ff6dcd8e444a2277 |
|---|---|
| oai_identifier_str |
oai:scielo:S0074-02761995000100012 |
| network_acronym_str |
FIOCRUZ-4 |
| network_name_str |
Memórias do Instituto Oswaldo Cruz |
| spelling |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infectionT cell-subpopulationsLeishmania m. mexicanaliposomes monoclonal antibodiesWe studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania.Instituto Oswaldo Cruz, Ministério da Saúde1995-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000100012Memórias do Instituto Oswaldo Cruz v.90 n.1 1995reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761995000100012info:eu-repo/semantics/openAccessLezama-Dávila,C. MGallagher,Geng2020-04-25T17:47:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:22.105Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
| dc.title.none.fl_str_mv |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| title |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| spellingShingle |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection Lezama-Dávila,C. M T cell-subpopulations Leishmania m. mexicana liposomes monoclonal antibodies |
| title_short |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| title_full |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| title_fullStr |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| title_full_unstemmed |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| title_sort |
CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection |
| author |
Lezama-Dávila,C. M |
| author_facet |
Lezama-Dávila,C. M Gallagher,G |
| author_role |
author |
| author2 |
Gallagher,G |
| author2_role |
author |
| dc.contributor.author.fl_str_mv |
Lezama-Dávila,C. M Gallagher,G |
| dc.subject.por.fl_str_mv |
T cell-subpopulations Leishmania m. mexicana liposomes monoclonal antibodies |
| topic |
T cell-subpopulations Leishmania m. mexicana liposomes monoclonal antibodies |
| dc.description.none.fl_txt_mv |
We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania. |
| description |
We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania. |
| publishDate |
1995 |
| dc.date.none.fl_str_mv |
1995-02-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000100012 |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000100012 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S0074-02761995000100012 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.90 n.1 1995 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
| reponame_str |
Memórias do Instituto Oswaldo Cruz |
| collection |
Memórias do Instituto Oswaldo Cruz |
| instname_str |
Fundação Oswaldo Cruz |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
| repository.mail.fl_str_mv |
|
| _version_ |
1669937663225888768 |