The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700009 |
Resumo: | Orally transmitted Chagas disease (ChD), which is a well-known entity in the Brazilian Amazon Region, was first documented in Venezuela in December 2007, when 103 people attending an urban public school in Caracas became infected by ingesting juice that was contaminated with Trypanosoma cruzi. The infection occurred 45-50 days prior to the initiation of the sampling performed in the current study. Parasitological methods were used to diagnose the first nine symptomatic patients; T. cruzi was found in all of them. However, because this outbreak was managed as a sudden emergency during Christmas time, we needed to rapidly evaluate 1,000 people at risk, so we decided to use conventional serology to detect specific IgM and IgG antibodies via ELISA as well as indirect haemagglutination, which produced positive test results for 9.1%, 11.9% and 9.9% of the individuals tested, respectively. In other more restricted patient groups, polymerase chain reaction (PCR) provided more sensitive results (80.4%) than blood cultures (16.2%) and animal inoculations (11.6%). Although the classical diagnosis of acute ChD is mainly based on parasitological findings, highly sensitive and specific serological techniques can provide rapid results during large and severe outbreaks, as described herein. The use of these serological techniques allows prompt treatment of all individuals suspected of being infected, resulting in reduced rates of morbidity and mortality. |
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Memórias do Instituto Oswaldo Cruz |
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The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas diseaseChagas diseaseoral transmissionoutbreakdiagnosisserologyVenezuelaOrally transmitted Chagas disease (ChD), which is a well-known entity in the Brazilian Amazon Region, was first documented in Venezuela in December 2007, when 103 people attending an urban public school in Caracas became infected by ingesting juice that was contaminated with Trypanosoma cruzi. The infection occurred 45-50 days prior to the initiation of the sampling performed in the current study. Parasitological methods were used to diagnose the first nine symptomatic patients; T. cruzi was found in all of them. However, because this outbreak was managed as a sudden emergency during Christmas time, we needed to rapidly evaluate 1,000 people at risk, so we decided to use conventional serology to detect specific IgM and IgG antibodies via ELISA as well as indirect haemagglutination, which produced positive test results for 9.1%, 11.9% and 9.9% of the individuals tested, respectively. In other more restricted patient groups, polymerase chain reaction (PCR) provided more sensitive results (80.4%) than blood cultures (16.2%) and animal inoculations (11.6%). Although the classical diagnosis of acute ChD is mainly based on parasitological findings, highly sensitive and specific serological techniques can provide rapid results during large and severe outbreaks, as described herein. The use of these serological techniques allows prompt treatment of all individuals suspected of being infected, resulting in reduced rates of morbidity and mortality.Instituto Oswaldo Cruz, Ministério da Saúde2012-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700009Memórias do Instituto Oswaldo Cruz v.107 n.7 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000700009info:eu-repo/semantics/openAccessNoya,Belkisyolé Alarcón deDíaz-Bello,ZoraidaColmenares,CeciliaZavala-Jaspe,ReinaldoAbate,TeresaContreras,RosaLosada,SandraArtigas,DomingoMauriello,LucianoRuiz-Guevara,RaizaNoya,Oscareng2020-04-25T17:51:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:34.047Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
title |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
spellingShingle |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease Noya,Belkisyolé Alarcón de Chagas disease oral transmission outbreak diagnosis serology Venezuela |
title_short |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
title_full |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
title_fullStr |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
title_full_unstemmed |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
title_sort |
The performance of laboratory tests in the management of a large outbreak of orally transmitted Chagas disease |
author |
Noya,Belkisyolé Alarcón de |
author_facet |
Noya,Belkisyolé Alarcón de Díaz-Bello,Zoraida Colmenares,Cecilia Zavala-Jaspe,Reinaldo Abate,Teresa Contreras,Rosa Losada,Sandra Artigas,Domingo Mauriello,Luciano Ruiz-Guevara,Raiza Noya,Oscar |
author_role |
author |
author2 |
Díaz-Bello,Zoraida Colmenares,Cecilia Zavala-Jaspe,Reinaldo Abate,Teresa Contreras,Rosa Losada,Sandra Artigas,Domingo Mauriello,Luciano Ruiz-Guevara,Raiza Noya,Oscar |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Noya,Belkisyolé Alarcón de Díaz-Bello,Zoraida Colmenares,Cecilia Zavala-Jaspe,Reinaldo Abate,Teresa Contreras,Rosa Losada,Sandra Artigas,Domingo Mauriello,Luciano Ruiz-Guevara,Raiza Noya,Oscar |
dc.subject.por.fl_str_mv |
Chagas disease oral transmission outbreak diagnosis serology Venezuela |
topic |
Chagas disease oral transmission outbreak diagnosis serology Venezuela |
dc.description.none.fl_txt_mv |
Orally transmitted Chagas disease (ChD), which is a well-known entity in the Brazilian Amazon Region, was first documented in Venezuela in December 2007, when 103 people attending an urban public school in Caracas became infected by ingesting juice that was contaminated with Trypanosoma cruzi. The infection occurred 45-50 days prior to the initiation of the sampling performed in the current study. Parasitological methods were used to diagnose the first nine symptomatic patients; T. cruzi was found in all of them. However, because this outbreak was managed as a sudden emergency during Christmas time, we needed to rapidly evaluate 1,000 people at risk, so we decided to use conventional serology to detect specific IgM and IgG antibodies via ELISA as well as indirect haemagglutination, which produced positive test results for 9.1%, 11.9% and 9.9% of the individuals tested, respectively. In other more restricted patient groups, polymerase chain reaction (PCR) provided more sensitive results (80.4%) than blood cultures (16.2%) and animal inoculations (11.6%). Although the classical diagnosis of acute ChD is mainly based on parasitological findings, highly sensitive and specific serological techniques can provide rapid results during large and severe outbreaks, as described herein. The use of these serological techniques allows prompt treatment of all individuals suspected of being infected, resulting in reduced rates of morbidity and mortality. |
description |
Orally transmitted Chagas disease (ChD), which is a well-known entity in the Brazilian Amazon Region, was first documented in Venezuela in December 2007, when 103 people attending an urban public school in Caracas became infected by ingesting juice that was contaminated with Trypanosoma cruzi. The infection occurred 45-50 days prior to the initiation of the sampling performed in the current study. Parasitological methods were used to diagnose the first nine symptomatic patients; T. cruzi was found in all of them. However, because this outbreak was managed as a sudden emergency during Christmas time, we needed to rapidly evaluate 1,000 people at risk, so we decided to use conventional serology to detect specific IgM and IgG antibodies via ELISA as well as indirect haemagglutination, which produced positive test results for 9.1%, 11.9% and 9.9% of the individuals tested, respectively. In other more restricted patient groups, polymerase chain reaction (PCR) provided more sensitive results (80.4%) than blood cultures (16.2%) and animal inoculations (11.6%). Although the classical diagnosis of acute ChD is mainly based on parasitological findings, highly sensitive and specific serological techniques can provide rapid results during large and severe outbreaks, as described herein. The use of these serological techniques allows prompt treatment of all individuals suspected of being infected, resulting in reduced rates of morbidity and mortality. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700009 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762012000700009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.107 n.7 2012 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937712924196864 |