Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain

Detalhes bibliográficos
Autor(a) principal: Le-Senne,Ana
Data de Publicação: 2002
Outros Autores: Muelas-Serrano,Susana, Fernández-Portillo,Carlos, Escario,José Antonio, Gómez-Barrio,Alicia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800006
Resumo: Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.
id FIOCRUZ-4_c329466118588eab0442c3b53196a736
oai_identifier_str oai:scielo:S0074-02762002000800006
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strainTrypanosoma cruziclone CL B5biological characterizationClone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.Instituto Oswaldo Cruz, Ministério da Saúde2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800006Memórias do Instituto Oswaldo Cruz v.97 n.8 2002reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762002000800006info:eu-repo/semantics/openAccessLe-Senne,AnaMuelas-Serrano,SusanaFernández-Portillo,CarlosEscario,José AntonioGómez-Barrio,Aliciaeng2020-04-25T17:48:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:28.321Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
title Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
spellingShingle Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
Le-Senne,Ana
Trypanosoma cruzi
clone CL B5
biological characterization
title_short Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
title_full Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
title_fullStr Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
title_full_unstemmed Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
title_sort Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
author Le-Senne,Ana
author_facet Le-Senne,Ana
Muelas-Serrano,Susana
Fernández-Portillo,Carlos
Escario,José Antonio
Gómez-Barrio,Alicia
author_role author
author2 Muelas-Serrano,Susana
Fernández-Portillo,Carlos
Escario,José Antonio
Gómez-Barrio,Alicia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Le-Senne,Ana
Muelas-Serrano,Susana
Fernández-Portillo,Carlos
Escario,José Antonio
Gómez-Barrio,Alicia
dc.subject.por.fl_str_mv Trypanosoma cruzi
clone CL B5
biological characterization
topic Trypanosoma cruzi
clone CL B5
biological characterization
dc.description.none.fl_txt_mv Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.
description Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.
publishDate 2002
dc.date.none.fl_str_mv 2002-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800006
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762002000800006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.97 n.8 2002
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937686799974400

Registros relacionados