Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures

Detalhes bibliográficos
Autor(a) principal: Abath,Frederico Guilherme Coutinho
Data de Publicação: 1990
Outros Autores: Almeida,Alzira Maria Paiva de, Ferreira,Luís Carlos de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100018
Resumo: Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.
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spelling Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperaturesYersinia pestisouter membrame proteinsThree Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.Instituto Oswaldo Cruz, Ministério da Saúde1990-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100018Memórias do Instituto Oswaldo Cruz v.85 n.1 1990reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761990000100018info:eu-repo/semantics/openAccessAbath,Frederico Guilherme CoutinhoAlmeida,Alzira Maria Paiva deFerreira,Luís Carlos de Souzaeng2020-04-25T17:46:28Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:03:40.067Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
title Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
spellingShingle Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
Abath,Frederico Guilherme Coutinho
Yersinia pestis
outer membrame proteins
title_short Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
title_full Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
title_fullStr Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
title_full_unstemmed Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
title_sort Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
author Abath,Frederico Guilherme Coutinho
author_facet Abath,Frederico Guilherme Coutinho
Almeida,Alzira Maria Paiva de
Ferreira,Luís Carlos de Souza
author_role author
author2 Almeida,Alzira Maria Paiva de
Ferreira,Luís Carlos de Souza
author2_role author
author
dc.contributor.author.fl_str_mv Abath,Frederico Guilherme Coutinho
Almeida,Alzira Maria Paiva de
Ferreira,Luís Carlos de Souza
dc.subject.por.fl_str_mv Yersinia pestis
outer membrame proteins
topic Yersinia pestis
outer membrame proteins
dc.description.none.fl_txt_mv Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.
description Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted.
publishDate 1990
dc.date.none.fl_str_mv 1990-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761990000100018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.85 n.1 1990
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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