Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology

Detalhes bibliográficos
Autor(a) principal: Abath, Frederico Guilherme Coutinho
Data de Publicação: 1990
Outros Autores: Ferreira, Luís Carlos de Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28714
Resumo: In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods resulted in similar protein patterns. The measurement of NADH-dehydrogenase and succinate dehydrogenase (inner membrane enzymes) indicated that the outer membrane preparations obtained by the three methods were pure enough for analytical studies. In addition, preliminary evidences on the potential use of outer membrane proteins for the identification of geographic variants of Y. pestis wild isolates are presented.
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spelling Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology Estudo comparativo de técnicas de isolamento de membrana externa de Yersinia pestis e seu uso na epidemiologia da peste Yersinia pestisouter membrane proteinsmembrane isolation techniques In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods resulted in similar protein patterns. The measurement of NADH-dehydrogenase and succinate dehydrogenase (inner membrane enzymes) indicated that the outer membrane preparations obtained by the three methods were pure enough for analytical studies. In addition, preliminary evidences on the potential use of outer membrane proteins for the identification of geographic variants of Y. pestis wild isolates are presented. No presente estudo três técnicas para isolamento de frações enriquecidas em membrana externa de Y. pestis foram avaliadas. As técnicas utilizadas foram: centrifugação em gradiente de densidade em sacarose e solubilização diferencial com Sarkosyl ou Triton X-100. A análise por eletroforese em gel de poliacrilamida na presença de dodecil sulfato de sódio (SDS-PAGE) das membranas externas extraídas pelos diferentes métodos evidenciou perfis protéicos semelhantes. A determinação das atividades de NADH-desidrogenase e succinato-desidrogenase (enzimas de membrana interna) indicou que todas as preparações estudadas eram adequadas a estudos analíticos. Obteve-se evidências preliminares sobre o possível uso de perfis protéicos de membrana externa na identificação de variantes geográficos entre isolados selvagens de Y. pestis. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1990-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28714Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 2 (1990); 78-83 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 2 (1990); 78-83 Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 2 (1990); 78-83 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28714/30567Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessAbath, Frederico Guilherme CoutinhoFerreira, Luís Carlos de Sousa2012-07-02T01:27:31Zoai:revistas.usp.br:article/28714Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:27.946144Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
Estudo comparativo de técnicas de isolamento de membrana externa de Yersinia pestis e seu uso na epidemiologia da peste
title Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
spellingShingle Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
Abath, Frederico Guilherme Coutinho
Yersinia pestis
outer membrane proteins
membrane isolation techniques
title_short Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
title_full Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
title_fullStr Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
title_full_unstemmed Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
title_sort Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology
author Abath, Frederico Guilherme Coutinho
author_facet Abath, Frederico Guilherme Coutinho
Ferreira, Luís Carlos de Sousa
author_role author
author2 Ferreira, Luís Carlos de Sousa
author2_role author
dc.contributor.author.fl_str_mv Abath, Frederico Guilherme Coutinho
Ferreira, Luís Carlos de Sousa
dc.subject.por.fl_str_mv Yersinia pestis
outer membrane proteins
membrane isolation techniques
topic Yersinia pestis
outer membrane proteins
membrane isolation techniques
description In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods resulted in similar protein patterns. The measurement of NADH-dehydrogenase and succinate dehydrogenase (inner membrane enzymes) indicated that the outer membrane preparations obtained by the three methods were pure enough for analytical studies. In addition, preliminary evidences on the potential use of outer membrane proteins for the identification of geographic variants of Y. pestis wild isolates are presented.
publishDate 1990
dc.date.none.fl_str_mv 1990-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28714
url https://www.revistas.usp.br/rimtsp/article/view/28714
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28714/30567
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 No. 2 (1990); 78-83
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 32 Núm. 2 (1990); 78-83
Revista do Instituto de Medicina Tropical de São Paulo; v. 32 n. 2 (1990); 78-83
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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