A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia

Detalhes bibliográficos
Autor(a) principal: Gallo,Stephanie Wagner
Data de Publicação: 2013
Outros Autores: Ramos,Patricia Locosque, Ferreira,Carlos Alexandre Sanchez, Oliveira,Silvia Dias de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300390
Resumo: Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
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spelling A specific polymerase chain reaction method to identify Stenotrophomonas maltophiliaStenotrophomonas maltophiliaidentificationPCR Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.Instituto Oswaldo Cruz, Ministério da Saúde2013-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300390Memórias do Instituto Oswaldo Cruz v.108 n.3 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762013000300020info:eu-repo/semantics/openAccessGallo,Stephanie WagnerRamos,Patricia LocosqueFerreira,Carlos Alexandre SanchezOliveira,Silvia Dias deeng2020-04-25T17:51:24Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:59.695Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
title A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
spellingShingle A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
Gallo,Stephanie Wagner
Stenotrophomonas maltophilia
identification
PCR
title_short A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
title_full A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
title_fullStr A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
title_full_unstemmed A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
title_sort A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
author Gallo,Stephanie Wagner
author_facet Gallo,Stephanie Wagner
Ramos,Patricia Locosque
Ferreira,Carlos Alexandre Sanchez
Oliveira,Silvia Dias de
author_role author
author2 Ramos,Patricia Locosque
Ferreira,Carlos Alexandre Sanchez
Oliveira,Silvia Dias de
author2_role author
author
author
dc.contributor.author.fl_str_mv Gallo,Stephanie Wagner
Ramos,Patricia Locosque
Ferreira,Carlos Alexandre Sanchez
Oliveira,Silvia Dias de
dc.subject.por.fl_str_mv Stenotrophomonas maltophilia
identification
PCR
topic Stenotrophomonas maltophilia
identification
PCR
dc.description.none.fl_txt_mv Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
description Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300390
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000300390
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762013000300020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.108 n.3 2013
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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