B-cell infiltration and frequency of cytokine producing cells differ between localized and disseminated human cutaneous leishmaniases

• Autor(a) principal: Vieira,MGS
• Data de Publicação: 2002
• Outros Autores: Oliveira,F, Arruda,S, Bittencourt,AL, Barbosa Jr,AA, Barral-Netto,M, Barral,A
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Memórias do Instituto Oswaldo Cruz
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000700009
• topic: leishmaniasis; B lymphocytes; cytokines; CD8+ T cells; IFN-<FONT FACE=Symbol>g</font>

Biopsies from human localized cutaneous lesions (LCL n = 7) or disseminated lesions (DL n = 8) cases were characterized according to cellular infiltration,frequency of cytokine (IFN-<FONT FACE=Symbol>g</font>, TNF-alpha) or iNOS enzyme producing cells. LCL, the most usual form of the disease with usually one or two lesions, exhibits extensive tissue damage. DL is a rare form with widespread lesions throughout the body; exhibiting poor parasite containment but less tissue damage. We demonstrated that LCL lesions exhibit higher frequency of B lymphocytes and a higher intensity of IFN-gamma expression. In both forms of the disease CD8+ were found in higher frequency than CD4+ T cells. Frequency of TNF-alpha and iNOS producing cells, as well as the frequency of CD68+ macrophages, did not differ between LCL and DL. Our findings reinforce the link between an efficient control of parasite and tissue damage, implicating higher frequency of IFN-gamma producing cells, as well as its possible counteraction by infiltrated B cells and hence possible humoral immune response in situ.