Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015 |
Resumo: | Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination. |
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Memórias do Instituto Oswaldo Cruz |
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Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samplesapoptosisParacoccidioides brasiliensiscaspase 3BakBcl-2Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.Instituto Oswaldo Cruz, Ministério da Saúde2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015Memórias do Instituto Oswaldo Cruz v.104 n.5 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000500015info:eu-repo/semantics/openAccessDel Vecchio,AdrianaSilva,Julhiany de Fatima daSilva,Juliana Leal Monteiro daAndreotti,Patricia FerrariSoares,Christiane PiennaBenard,GilGiannini,Maria José Soares Mendeseng2020-04-25T17:50:31Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:21.293Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
title |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
spellingShingle |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples Del Vecchio,Adriana apoptosis Paracoccidioides brasiliensis caspase 3 Bak Bcl-2 |
title_short |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
title_full |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
title_fullStr |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
title_full_unstemmed |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
title_sort |
Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples |
author |
Del Vecchio,Adriana |
author_facet |
Del Vecchio,Adriana Silva,Julhiany de Fatima da Silva,Juliana Leal Monteiro da Andreotti,Patricia Ferrari Soares,Christiane Pienna Benard,Gil Giannini,Maria José Soares Mendes |
author_role |
author |
author2 |
Silva,Julhiany de Fatima da Silva,Juliana Leal Monteiro da Andreotti,Patricia Ferrari Soares,Christiane Pienna Benard,Gil Giannini,Maria José Soares Mendes |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Del Vecchio,Adriana Silva,Julhiany de Fatima da Silva,Juliana Leal Monteiro da Andreotti,Patricia Ferrari Soares,Christiane Pienna Benard,Gil Giannini,Maria José Soares Mendes |
dc.subject.por.fl_str_mv |
apoptosis Paracoccidioides brasiliensis caspase 3 Bak Bcl-2 |
topic |
apoptosis Paracoccidioides brasiliensis caspase 3 Bak Bcl-2 |
dc.description.none.fl_txt_mv |
Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination. |
description |
Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762009000500015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.104 n.5 2009 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937705536978944 |