Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples

Detalhes bibliográficos
Autor(a) principal: Del Vecchio,Adriana
Data de Publicação: 2009
Outros Autores: Silva,Julhiany de Fatima da, Silva,Juliana Leal Monteiro da, Andreotti,Patricia Ferrari, Soares,Christiane Pienna, Benard,Gil, Giannini,Maria José Soares Mendes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015
Resumo: Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.
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spelling Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samplesapoptosisParacoccidioides brasiliensiscaspase 3BakBcl-2Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.Instituto Oswaldo Cruz, Ministério da Saúde2009-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015Memórias do Instituto Oswaldo Cruz v.104 n.5 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000500015info:eu-repo/semantics/openAccessDel Vecchio,AdrianaSilva,Julhiany de Fatima daSilva,Juliana Leal Monteiro daAndreotti,Patricia FerrariSoares,Christiane PiennaBenard,GilGiannini,Maria José Soares Mendeseng2020-04-25T17:50:31Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:21.293Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
title Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
spellingShingle Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
Del Vecchio,Adriana
apoptosis
Paracoccidioides brasiliensis
caspase 3
Bak
Bcl-2
title_short Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
title_full Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
title_fullStr Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
title_full_unstemmed Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
title_sort Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
author Del Vecchio,Adriana
author_facet Del Vecchio,Adriana
Silva,Julhiany de Fatima da
Silva,Juliana Leal Monteiro da
Andreotti,Patricia Ferrari
Soares,Christiane Pienna
Benard,Gil
Giannini,Maria José Soares Mendes
author_role author
author2 Silva,Julhiany de Fatima da
Silva,Juliana Leal Monteiro da
Andreotti,Patricia Ferrari
Soares,Christiane Pienna
Benard,Gil
Giannini,Maria José Soares Mendes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Del Vecchio,Adriana
Silva,Julhiany de Fatima da
Silva,Juliana Leal Monteiro da
Andreotti,Patricia Ferrari
Soares,Christiane Pienna
Benard,Gil
Giannini,Maria José Soares Mendes
dc.subject.por.fl_str_mv apoptosis
Paracoccidioides brasiliensis
caspase 3
Bak
Bcl-2
topic apoptosis
Paracoccidioides brasiliensis
caspase 3
Bak
Bcl-2
dc.description.none.fl_txt_mv Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.
description Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.
publishDate 2009
dc.date.none.fl_str_mv 2009-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000500015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762009000500015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.104 n.5 2009
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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