Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

Detalhes bibliográficos
Autor(a) principal: Rubiano,Claudia C
Data de Publicação: 2003
Outros Autores: Wasserman,Moises
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018
Resumo: A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
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spelling Detection of telomerase activity in Plasmodium falciparum using a nonradioactive methodPlasmodiummalariatelomeraseassaynonradioactiveA simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.Instituto Oswaldo Cruz, Ministério da Saúde2003-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018Memórias do Instituto Oswaldo Cruz v.98 n.5 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000500018info:eu-repo/semantics/openAccessRubiano,Claudia CWasserman,Moiseseng2020-04-25T17:49:06Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:05.311Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
spellingShingle Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
Rubiano,Claudia C
Plasmodium
malaria
telomerase
assay
nonradioactive
title_short Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_full Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_fullStr Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_full_unstemmed Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
title_sort Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method
author Rubiano,Claudia C
author_facet Rubiano,Claudia C
Wasserman,Moises
author_role author
author2 Wasserman,Moises
author2_role author
dc.contributor.author.fl_str_mv Rubiano,Claudia C
Wasserman,Moises
dc.subject.por.fl_str_mv Plasmodium
malaria
telomerase
assay
nonradioactive
topic Plasmodium
malaria
telomerase
assay
nonradioactive
dc.description.none.fl_txt_mv A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
description A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
publishDate 2003
dc.date.none.fl_str_mv 2003-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000500018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762003000500018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.98 n.5 2003
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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