Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300011 |
Resumo: | The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests. |
id |
FIOCRUZ-4_d038e1548cc8f1124e179b993252a653 |
---|---|
oai_identifier_str |
oai:scielo:S0074-02762010000300011 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in childrenleishmaniasisPCRperipheral bloodchildrenThe diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.Instituto Oswaldo Cruz, Ministério da Saúde2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300011Memórias do Instituto Oswaldo Cruz v.105 n.3 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000300011info:eu-repo/semantics/openAccessFraga,Thiago LeiteBrustoloni,Yvone MaiaLima,Rosimar BaptistaDorval,Maria Elizabeth CavalheirosOshiro,Elisa TeruyaOliveira,JanainaOliveira,Ana Lúcia Lyrio dePirmez,Claudeeng2020-04-25T17:50:47Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:55.037Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
title |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
spellingShingle |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children Fraga,Thiago Leite leishmaniasis PCR peripheral blood children |
title_short |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
title_full |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
title_fullStr |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
title_full_unstemmed |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
title_sort |
Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children |
author |
Fraga,Thiago Leite |
author_facet |
Fraga,Thiago Leite Brustoloni,Yvone Maia Lima,Rosimar Baptista Dorval,Maria Elizabeth Cavalheiros Oshiro,Elisa Teruya Oliveira,Janaina Oliveira,Ana Lúcia Lyrio de Pirmez,Claude |
author_role |
author |
author2 |
Brustoloni,Yvone Maia Lima,Rosimar Baptista Dorval,Maria Elizabeth Cavalheiros Oshiro,Elisa Teruya Oliveira,Janaina Oliveira,Ana Lúcia Lyrio de Pirmez,Claude |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fraga,Thiago Leite Brustoloni,Yvone Maia Lima,Rosimar Baptista Dorval,Maria Elizabeth Cavalheiros Oshiro,Elisa Teruya Oliveira,Janaina Oliveira,Ana Lúcia Lyrio de Pirmez,Claude |
dc.subject.por.fl_str_mv |
leishmaniasis PCR peripheral blood children |
topic |
leishmaniasis PCR peripheral blood children |
dc.description.none.fl_txt_mv |
The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests. |
description |
The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300011 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762010000300011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.105 n.3 2010 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937707861671936 |