Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007 |
Resumo: | Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed. |
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Memórias do Instituto Oswaldo Cruz |
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Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cellsTrypanosoma cruziphenothiazinescalcium antagonistsparasite-host cell interactionsPhenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed.Instituto Oswaldo Cruz, Ministério da Saúde1992-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007Memórias do Instituto Oswaldo Cruz v.87 n.2 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000200007info:eu-repo/semantics/openAccessCastro,Solange L. deSoeiro,Maria Nazaré C.Meirelles,Maria de Nazareth Leal deeng2020-04-25T17:46:43Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:35.546Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
title |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
spellingShingle |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells Castro,Solange L. de Trypanosoma cruzi phenothiazines calcium antagonists parasite-host cell interactions |
title_short |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
title_full |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
title_fullStr |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
title_full_unstemmed |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
title_sort |
Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells |
author |
Castro,Solange L. de |
author_facet |
Castro,Solange L. de Soeiro,Maria Nazaré C. Meirelles,Maria de Nazareth Leal de |
author_role |
author |
author2 |
Soeiro,Maria Nazaré C. Meirelles,Maria de Nazareth Leal de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Castro,Solange L. de Soeiro,Maria Nazaré C. Meirelles,Maria de Nazareth Leal de |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi phenothiazines calcium antagonists parasite-host cell interactions |
topic |
Trypanosoma cruzi phenothiazines calcium antagonists parasite-host cell interactions |
dc.description.none.fl_txt_mv |
Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed. |
description |
Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761992000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.87 n.2 1992 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937658299678720 |