Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil

Detalhes bibliográficos
Autor(a) principal: Gardinali,Noemi Rovaris
Data de Publicação: 2012
Outros Autores: Barry,Aline Fernandes, Otonel,Rodrigo Alejandro Arellano, Alfieri,Alice Fernandes, Alfieri,Amauri Alcindo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700016
Resumo: The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.
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spelling Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazilhepatitis E virusswineslaughterhouseThe objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.Instituto Oswaldo Cruz, Ministério da Saúde2012-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700016Memórias do Instituto Oswaldo Cruz v.107 n.7 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000700016info:eu-repo/semantics/openAccessGardinali,Noemi RovarisBarry,Aline FernandesOtonel,Rodrigo Alejandro ArellanoAlfieri,Alice FernandesAlfieri,Amauri Alcindoeng2020-04-25T17:51:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:35.409Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
title Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
spellingShingle Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
Gardinali,Noemi Rovaris
hepatitis E virus
swine
slaughterhouse
title_short Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
title_full Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
title_fullStr Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
title_full_unstemmed Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
title_sort Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil
author Gardinali,Noemi Rovaris
author_facet Gardinali,Noemi Rovaris
Barry,Aline Fernandes
Otonel,Rodrigo Alejandro Arellano
Alfieri,Alice Fernandes
Alfieri,Amauri Alcindo
author_role author
author2 Barry,Aline Fernandes
Otonel,Rodrigo Alejandro Arellano
Alfieri,Alice Fernandes
Alfieri,Amauri Alcindo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gardinali,Noemi Rovaris
Barry,Aline Fernandes
Otonel,Rodrigo Alejandro Arellano
Alfieri,Alice Fernandes
Alfieri,Amauri Alcindo
dc.subject.por.fl_str_mv hepatitis E virus
swine
slaughterhouse
topic hepatitis E virus
swine
slaughterhouse
dc.description.none.fl_txt_mv The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.
description The objective of this study was to detect and identify hepatitis E virus (HEV) strains in liver and bile samples from slaughtered pigs in the state of Paraná, Brazil. Liver and bile samples were collected from 118 asymptomatic adult pigs at a slaughterhouse in a major Brazilian pork production area. The samples were assayed using a nested reverse transcription-polymerase chain reaction protocol with primer sets targeting open reading frames (ORF)1 and 2 of the HEV genome. HEV RNA was detected in two (1.7%) liver samples and one (0.84%) bile sample using both primers sets. The HEV strains were classified as genotype 3b on the basis of their nucleotide sequences. These data suggest that healthy pigs may be a source of HEV infection for consumers of pig liver and slaughterhouse workers in Brazil.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762012000700016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.107 n.7 2012
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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