Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil

Detalhes bibliográficos
Autor(a) principal: Bueris,Vanessa
Data de Publicação: 2007
Outros Autores: Sircili,Marcelo Palma, Taddei,Carla Romano, Santos,Maurilio Fernandes dos, Franzolin,Marcia Regina, Martinez,Marina Baquerizo, Ferrer,Suzana Ramos, Barreto,Mauricio Lima, Trabulsi,Luiz Rachid
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009
Resumo: We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.
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spelling Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazildiarrheagenic Escherichia colivirulenceMultiplex PCRWe identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.Instituto Oswaldo Cruz, Ministério da Saúde2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009Memórias do Instituto Oswaldo Cruz v.102 n.7 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007005000116info:eu-repo/semantics/openAccessBueris,VanessaSircili,Marcelo PalmaTaddei,Carla RomanoSantos,Maurilio Fernandes dosFranzolin,Marcia ReginaMartinez,Marina BaquerizoFerrer,Suzana RamosBarreto,Mauricio LimaTrabulsi,Luiz Rachideng2020-04-25T17:50:13Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:23.779Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
title Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
spellingShingle Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
Bueris,Vanessa
diarrheagenic Escherichia coli
virulence
Multiplex PCR
title_short Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
title_full Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
title_fullStr Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
title_full_unstemmed Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
title_sort Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
author Bueris,Vanessa
author_facet Bueris,Vanessa
Sircili,Marcelo Palma
Taddei,Carla Romano
Santos,Maurilio Fernandes dos
Franzolin,Marcia Regina
Martinez,Marina Baquerizo
Ferrer,Suzana Ramos
Barreto,Mauricio Lima
Trabulsi,Luiz Rachid
author_role author
author2 Sircili,Marcelo Palma
Taddei,Carla Romano
Santos,Maurilio Fernandes dos
Franzolin,Marcia Regina
Martinez,Marina Baquerizo
Ferrer,Suzana Ramos
Barreto,Mauricio Lima
Trabulsi,Luiz Rachid
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bueris,Vanessa
Sircili,Marcelo Palma
Taddei,Carla Romano
Santos,Maurilio Fernandes dos
Franzolin,Marcia Regina
Martinez,Marina Baquerizo
Ferrer,Suzana Ramos
Barreto,Mauricio Lima
Trabulsi,Luiz Rachid
dc.subject.por.fl_str_mv diarrheagenic Escherichia coli
virulence
Multiplex PCR
topic diarrheagenic Escherichia coli
virulence
Multiplex PCR
dc.description.none.fl_txt_mv We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.
description We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762007005000116
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.102 n.7 2007
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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