Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009 |
Resumo: | We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea. |
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Memórias do Instituto Oswaldo Cruz |
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Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazildiarrheagenic Escherichia colivirulenceMultiplex PCRWe identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.Instituto Oswaldo Cruz, Ministério da Saúde2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009Memórias do Instituto Oswaldo Cruz v.102 n.7 2007reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762007005000116info:eu-repo/semantics/openAccessBueris,VanessaSircili,Marcelo PalmaTaddei,Carla RomanoSantos,Maurilio Fernandes dosFranzolin,Marcia ReginaMartinez,Marina BaquerizoFerrer,Suzana RamosBarreto,Mauricio LimaTrabulsi,Luiz Rachideng2020-04-25T17:50:13Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:23.779Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
title |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
spellingShingle |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil Bueris,Vanessa diarrheagenic Escherichia coli virulence Multiplex PCR |
title_short |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
title_full |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
title_fullStr |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
title_full_unstemmed |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
title_sort |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
author |
Bueris,Vanessa |
author_facet |
Bueris,Vanessa Sircili,Marcelo Palma Taddei,Carla Romano Santos,Maurilio Fernandes dos Franzolin,Marcia Regina Martinez,Marina Baquerizo Ferrer,Suzana Ramos Barreto,Mauricio Lima Trabulsi,Luiz Rachid |
author_role |
author |
author2 |
Sircili,Marcelo Palma Taddei,Carla Romano Santos,Maurilio Fernandes dos Franzolin,Marcia Regina Martinez,Marina Baquerizo Ferrer,Suzana Ramos Barreto,Mauricio Lima Trabulsi,Luiz Rachid |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bueris,Vanessa Sircili,Marcelo Palma Taddei,Carla Romano Santos,Maurilio Fernandes dos Franzolin,Marcia Regina Martinez,Marina Baquerizo Ferrer,Suzana Ramos Barreto,Mauricio Lima Trabulsi,Luiz Rachid |
dc.subject.por.fl_str_mv |
diarrheagenic Escherichia coli virulence Multiplex PCR |
topic |
diarrheagenic Escherichia coli virulence Multiplex PCR |
dc.description.none.fl_txt_mv |
We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea. |
description |
We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762007005000116 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.102 n.7 2007 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937701851234304 |