Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/1551 |
Resumo: | p. 839-844 |
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Bueris, VanessaSircili, Marcelo PalmaTaddei, Carla RomanoSantos, Maurilio Fernandes dosFranzolin, Marcia ReginaMartinez, Marina BaquerizoFerrer, Suzana RamosBarreto, Mauricio LimaTrabulsi, Luiz RachidBueris, VanessaSircili, Marcelo PalmaTaddei, Carla RomanoSantos, Maurilio Fernandes dosFranzolin, Marcia ReginaMartinez, Marina BaquerizoFerrer, Suzana RamosBarreto, Mauricio LimaTrabulsi, Luiz Rachid2011-06-18T04:10:13Z2011-06-18T04:10:13Z20071678-8060http://www.repositorio.ufba.br/ri/handle/ri/1551102(7)p. 839-844We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador, Bahia, Brazil collected as part of a case control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/ Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEC) and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1,020 patients) and the control group (187 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.Submitted by Rodrigo Meirelles (rodrigomei@ufba.br) on 2011-06-18T04:10:13Z No. of bitstreams: 1 arrtigo repositorio16.pdf: 168125 bytes, checksum: 9d675bf2b299d3d9f1fe092ef087082d (MD5)Made available in DSpace on 2011-06-18T04:10:13Z (GMT). No. of bitstreams: 1 arrtigo repositorio16.pdf: 168125 bytes, checksum: 9d675bf2b299d3d9f1fe092ef087082d (MD5) Previous issue date: 2007Rio de Janeirodiarrheagenic Escherichia colivirulenceMultiplex PCRDetection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, BrazilMem Inst Oswaldo Cruzinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALarrtigo repositorio16.pdfarrtigo repositorio16.pdfapplication/pdf168125https://repositorio.ufba.br/bitstream/ri/1551/1/arrtigo%20repositorio16.pdf9d675bf2b299d3d9f1fe092ef087082dMD51LICENSElicense.txtlicense.txttext/plain1900https://repositorio.ufba.br/bitstream/ri/1551/2/license.txtcc5c844f708344a6d46faa7e8ef5b616MD52TEXTarrtigo repositorio16.pdf.txtarrtigo repositorio16.pdf.txtExtracted texttext/plain28675https://repositorio.ufba.br/bitstream/ri/1551/3/arrtigo%20repositorio16.pdf.txtcd71968fb534000043cd382f80dbe5d4MD53ri/15512022-07-05 14:03:28.306oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:28Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| dc.title.alternative.pt_BR.fl_str_mv |
Mem Inst Oswaldo Cruz |
| title |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| spellingShingle |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil Bueris, Vanessa diarrheagenic Escherichia coli virulence Multiplex PCR |
| title_short |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| title_full |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| title_fullStr |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| title_full_unstemmed |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| title_sort |
Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil |
| author |
Bueris, Vanessa |
| author_facet |
Bueris, Vanessa Sircili, Marcelo Palma Taddei, Carla Romano Santos, Maurilio Fernandes dos Franzolin, Marcia Regina Martinez, Marina Baquerizo Ferrer, Suzana Ramos Barreto, Mauricio Lima Trabulsi, Luiz Rachid |
| author_role |
author |
| author2 |
Sircili, Marcelo Palma Taddei, Carla Romano Santos, Maurilio Fernandes dos Franzolin, Marcia Regina Martinez, Marina Baquerizo Ferrer, Suzana Ramos Barreto, Mauricio Lima Trabulsi, Luiz Rachid |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bueris, Vanessa Sircili, Marcelo Palma Taddei, Carla Romano Santos, Maurilio Fernandes dos Franzolin, Marcia Regina Martinez, Marina Baquerizo Ferrer, Suzana Ramos Barreto, Mauricio Lima Trabulsi, Luiz Rachid Bueris, Vanessa Sircili, Marcelo Palma Taddei, Carla Romano Santos, Maurilio Fernandes dos Franzolin, Marcia Regina Martinez, Marina Baquerizo Ferrer, Suzana Ramos Barreto, Mauricio Lima Trabulsi, Luiz Rachid |
| dc.subject.por.fl_str_mv |
diarrheagenic Escherichia coli virulence Multiplex PCR |
| topic |
diarrheagenic Escherichia coli virulence Multiplex PCR |
| description |
p. 839-844 |
| publishDate |
2007 |
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2007 |
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2011-06-18T04:10:13Z |
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1678-8060 |
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102(7) |
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