Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174 |
Resumo: | Chagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels. |
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Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas diseasemetallothionein-Iantioxidantsnitric oxideL-NAMEChagas diseaseChagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels.Instituto Oswaldo Cruz, Ministério da Saúde2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174Memórias do Instituto Oswaldo Cruz v.109 n.2 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276140339info:eu-repo/semantics/openAccessGonzalez-Mejia,Martha ElbaTorres-Rasgado,EnriquePorchia,Leonardo MSalgado,Hilda RosasTotolhua,José-LuisOrtega,ArturoHernández-Kelly,Luisa Clara ReginaRuiz-Vivanco,GuadalupeBáez-Duarte,Blanca GPérez-Fuentes,Ricardoeng2020-04-25T17:51:53Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:11.86Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
title |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
spellingShingle |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease Gonzalez-Mejia,Martha Elba metallothionein-I antioxidants nitric oxide L-NAME Chagas disease |
title_short |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
title_full |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
title_fullStr |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
title_full_unstemmed |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
title_sort |
Metallothionein-1 and nitric oxide expression are inversely correlated in a murine model of Chagas disease |
author |
Gonzalez-Mejia,Martha Elba |
author_facet |
Gonzalez-Mejia,Martha Elba Torres-Rasgado,Enrique Porchia,Leonardo M Salgado,Hilda Rosas Totolhua,José-Luis Ortega,Arturo Hernández-Kelly,Luisa Clara Regina Ruiz-Vivanco,Guadalupe Báez-Duarte,Blanca G Pérez-Fuentes,Ricardo |
author_role |
author |
author2 |
Torres-Rasgado,Enrique Porchia,Leonardo M Salgado,Hilda Rosas Totolhua,José-Luis Ortega,Arturo Hernández-Kelly,Luisa Clara Regina Ruiz-Vivanco,Guadalupe Báez-Duarte,Blanca G Pérez-Fuentes,Ricardo |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gonzalez-Mejia,Martha Elba Torres-Rasgado,Enrique Porchia,Leonardo M Salgado,Hilda Rosas Totolhua,José-Luis Ortega,Arturo Hernández-Kelly,Luisa Clara Regina Ruiz-Vivanco,Guadalupe Báez-Duarte,Blanca G Pérez-Fuentes,Ricardo |
dc.subject.por.fl_str_mv |
metallothionein-I antioxidants nitric oxide L-NAME Chagas disease |
topic |
metallothionein-I antioxidants nitric oxide L-NAME Chagas disease |
dc.description.none.fl_txt_mv |
Chagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels. |
description |
Chagas disease, caused by Trypanosoma cruzi, represents an endemic among Latin America countries. The participation of free radicals, especially nitric oxide (NO), has been demonstrated in the pathophysiology of seropositive individuals with T. cruzi. In Chagas disease, increased NO contributes to the development of cardiomyopathy and megacolon. Metallothioneins (MTs) are efficient free radicals scavengers of NO in vitro and in vivo. Here, we developed a murine model of the chronic phase of Chagas disease using endemic T. cruzi RyCH1 in BALB/c mice, which were divided into four groups: infected non-treated (Inf), infected N-monomethyl-L-arginine treated (Inf L-NAME), non-infected L-NAME treated and non-infected vehicle-treated. We determined blood parasitaemia and NO levels, the extent of parasite nests in tissues and liver MT-I expression levels. It was observed that NO levels were increasing in Inf mice in a time-dependent manner. Inf L-NAME mice had fewer T. cruzi nests in cardiac and skeletal muscle with decreased blood NO levels at day 135 post infection. This affect was negatively correlated with an increase of MT-I expression (r = -0.8462, p < 0.0001). In conclusion, we determined that in Chagas disease, an unknown inhibitory mechanism reduces MT-I expression, allowing augmented NO levels. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000200174 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-0276140339 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.109 n.2 2014 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937717137375232 |