Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies

Detalhes bibliográficos
Autor(a) principal: Figueiredo,Luiz Tadeu Moraes
Data de Publicação: 1989
Outros Autores: Nogueira,Rita Maria Ribeiro, Cavalcanti,Silvia Maria Baêta, Schatzmayr,Hermann, Rosa,Amélia Travassos da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300003
Resumo: This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
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spelling Study of two different enzyme immunoassays for the detection of Mayaro virus antibodiesMayaroalphavirusarbovirusenzyme immunoassayThis paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.Instituto Oswaldo Cruz, Ministério da Saúde1989-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300003Memórias do Instituto Oswaldo Cruz v.84 n.3 1989reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761989000300003info:eu-repo/semantics/openAccessFigueiredo,Luiz Tadeu MoraesNogueira,Rita Maria RibeiroCavalcanti,Silvia Maria BaêtaSchatzmayr,HermannRosa,Amélia Travassos daeng2020-04-25T17:46:16Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:58.664Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
title Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
spellingShingle Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
Figueiredo,Luiz Tadeu Moraes
Mayaro
alphavirus
arbovirus
enzyme immunoassay
title_short Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
title_full Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
title_fullStr Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
title_full_unstemmed Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
title_sort Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies
author Figueiredo,Luiz Tadeu Moraes
author_facet Figueiredo,Luiz Tadeu Moraes
Nogueira,Rita Maria Ribeiro
Cavalcanti,Silvia Maria Baêta
Schatzmayr,Hermann
Rosa,Amélia Travassos da
author_role author
author2 Nogueira,Rita Maria Ribeiro
Cavalcanti,Silvia Maria Baêta
Schatzmayr,Hermann
Rosa,Amélia Travassos da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Figueiredo,Luiz Tadeu Moraes
Nogueira,Rita Maria Ribeiro
Cavalcanti,Silvia Maria Baêta
Schatzmayr,Hermann
Rosa,Amélia Travassos da
dc.subject.por.fl_str_mv Mayaro
alphavirus
arbovirus
enzyme immunoassay
topic Mayaro
alphavirus
arbovirus
enzyme immunoassay
dc.description.none.fl_txt_mv This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
description This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells ara used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent mayaro infection. IgG-ICC showed hight sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by ELISA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.
publishDate 1989
dc.date.none.fl_str_mv 1989-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300003
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761989000300003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761989000300003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.84 n.3 1989
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
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reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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