Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes

Detalhes bibliográficos
Autor(a) principal: Marinho,Fernanda de Aquino
Data de Publicação: 2011
Outros Autores: Gonçalves,Keyla Cristiny da Silva, Oliveira,Selma Soares de, Oliveira,Ana-Carolina de Siqueira Couto de, Bellio,Maria, d'Avila-Levy,Claudia Masini, Santos,André Luis Souza dos, Branquinha,Marta Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021
Resumo: In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.
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spelling Miltefosine induces programmed cell death in Leishmania amazonensis promastigotesleishmaniasischemotherapyapoptosisIn the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.Instituto Oswaldo Cruz, Ministério da Saúde2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021Memórias do Instituto Oswaldo Cruz v.106 n.4 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000400021info:eu-repo/semantics/openAccessMarinho,Fernanda de AquinoGonçalves,Keyla Cristiny da SilvaOliveira,Selma Soares deOliveira,Ana-Carolina de Siqueira Couto deBellio,Mariad'Avila-Levy,Claudia MasiniSantos,André Luis Souza dosBranquinha,Marta Helenaeng2020-04-25T17:51:01Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:43.193Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
title Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
spellingShingle Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
Marinho,Fernanda de Aquino
leishmaniasis
chemotherapy
apoptosis
title_short Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
title_full Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
title_fullStr Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
title_full_unstemmed Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
title_sort Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
author Marinho,Fernanda de Aquino
author_facet Marinho,Fernanda de Aquino
Gonçalves,Keyla Cristiny da Silva
Oliveira,Selma Soares de
Oliveira,Ana-Carolina de Siqueira Couto de
Bellio,Maria
d'Avila-Levy,Claudia Masini
Santos,André Luis Souza dos
Branquinha,Marta Helena
author_role author
author2 Gonçalves,Keyla Cristiny da Silva
Oliveira,Selma Soares de
Oliveira,Ana-Carolina de Siqueira Couto de
Bellio,Maria
d'Avila-Levy,Claudia Masini
Santos,André Luis Souza dos
Branquinha,Marta Helena
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marinho,Fernanda de Aquino
Gonçalves,Keyla Cristiny da Silva
Oliveira,Selma Soares de
Oliveira,Ana-Carolina de Siqueira Couto de
Bellio,Maria
d'Avila-Levy,Claudia Masini
Santos,André Luis Souza dos
Branquinha,Marta Helena
dc.subject.por.fl_str_mv leishmaniasis
chemotherapy
apoptosis
topic leishmaniasis
chemotherapy
apoptosis
dc.description.none.fl_txt_mv In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.
description In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000400021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.4 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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