Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni

Detalhes bibliográficos
Autor(a) principal: Rabello,Ana Lúcia Teles
Data de Publicação: 1992
Outros Autores: Dias Neto,Emmanuel, Garcia,Maria Monica Aguiar, Katz,Naftale
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003
Resumo: A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested: ELISA x dot-ELISA: 95.1%, ELISA x dot-DIA: 92.7% and dot-ELISA x dot-DIA: 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis.
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spelling Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoniSchistosomiasisimmunoassaydot-ELISAdot-DIAA new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested: ELISA x dot-ELISA: 95.1%, ELISA x dot-DIA: 92.7% and dot-ELISA x dot-DIA: 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis.Instituto Oswaldo Cruz, Ministério da Saúde1992-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003Memórias do Instituto Oswaldo Cruz v.87 n.2 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000200003info:eu-repo/semantics/openAccessRabello,Ana Lúcia TelesDias Neto,EmmanuelGarcia,Maria Monica AguiarKatz,Naftaleeng2020-04-25T17:46:43Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:34.78Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
title Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
spellingShingle Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
Rabello,Ana Lúcia Teles
Schistosomiasis
immunoassay
dot-ELISA
dot-DIA
title_short Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
title_full Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
title_fullStr Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
title_full_unstemmed Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
title_sort Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
author Rabello,Ana Lúcia Teles
author_facet Rabello,Ana Lúcia Teles
Dias Neto,Emmanuel
Garcia,Maria Monica Aguiar
Katz,Naftale
author_role author
author2 Dias Neto,Emmanuel
Garcia,Maria Monica Aguiar
Katz,Naftale
author2_role author
author
author
dc.contributor.author.fl_str_mv Rabello,Ana Lúcia Teles
Dias Neto,Emmanuel
Garcia,Maria Monica Aguiar
Katz,Naftale
dc.subject.por.fl_str_mv Schistosomiasis
immunoassay
dot-ELISA
dot-DIA
topic Schistosomiasis
immunoassay
dot-ELISA
dot-DIA
dc.description.none.fl_txt_mv A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested: ELISA x dot-ELISA: 95.1%, ELISA x dot-DIA: 92.7% and dot-ELISA x dot-DIA: 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis.
description A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps: (a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested: ELISA x dot-ELISA: 95.1%, ELISA x dot-DIA: 92.7% and dot-ELISA x dot-DIA: 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis.
publishDate 1992
dc.date.none.fl_str_mv 1992-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 n.2 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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