Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
Autor(a) principal: | |
---|---|
Data de Publicação: | 1995 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/29245 |
Resumo: | Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies. |
id |
IMT-1_4cdf84eed32dd5669cef43ba9522ae14 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/29245 |
network_acronym_str |
IMT-1 |
network_name_str |
Revista do Instituto de Medicina Tropical de São Paulo |
repository_id_str |
|
spelling |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes SchistosomiasisDOT-ELISAEgg excretionDiagnosis Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies. A esquistosomose humana, causada pelo Schistosoma mansoni, é altamente prevalente no Brasil e é usualmente diagnosticada através de exames de fezes, que são demorados. Os testes sorológicos nesta doença limitam-se aos estudos epidemiológicos, mostrando apenas o contato prévio com o parasita e não a infecção realmente ativa. Neste trabalho, nós padronizamos e comparamos um novo ensaio de Dot-ELISA, para detecção de IgM e IgG específicas contra antígenos de ovo e vermes de S. mansoni, comparando seus resultados com a técnica clássica de imunofluorescência (IFI), tanto para IgG ou IgM, utilizando antígenos similares, no estudo de uma população de 27 pacientes com quantificação de ovos nas fezes. A positividade do teste de Dot-ELISA foi de 96,3% para IgG e 88,9% para IgM, com antígeno de verme, e 92,6% para IgG e 90,9% para IgM com antígeno de ovo, que foi comparado com a IFI que apresentou resultados similares com antígeno de verme, 92,6% (IgG) e 96,3% (IgM) e menores com antígeno de ovo, 77,8% (IgG e IgM). Nosso grupo de pacientes claramente apresentava dois subgrupos, quanto à excreção de ovos nas fezes, com maior positividade dos testes sorológicos para o grupo composto por pacientes com maior excreção fecal de ovos. Quando comparamos a excreção quantitativa de ovos nas fezes com os títulos sorológicos, detectamos apenas correlação entre estes valores e o Dot-ELISA IgM (r=0,552 p=0.0127). Estes dados mostram que o teste de Dot-ELISA pode ser usado para a detecção de anticorpos específicos contra o S. mansoni em soros de pacientes suspeitos, para estudos epidemiológicos de prevalência; e, com maior purificação dos antígenos de ovos e amostragem ampliada, poderá tornar-se um teste útil para uma estimativa grosseira da carga parasitária em estudos epidemiológicos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29245Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 2 (1995); 109-115 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 2 (1995); 109-115 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 2 (1995); 109-115 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29245/31102Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessPinto, Pedro Luiz SilvaKanamura, Herminia Y.Silva, Rita MariaRossi, Cristina Renata NardotoAndrade Jr., Heitor Franco deAmato Neto, Vicente2012-07-02T01:39:50Zoai:revistas.usp.br:article/29245Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:59.712484Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true |
dc.title.none.fl_str_mv |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes |
title |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients |
spellingShingle |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients Pinto, Pedro Luiz Silva Schistosomiasis DOT-ELISA Egg excretion Diagnosis |
title_short |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients |
title_full |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients |
title_fullStr |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients |
title_full_unstemmed |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients |
title_sort |
Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients |
author |
Pinto, Pedro Luiz Silva |
author_facet |
Pinto, Pedro Luiz Silva Kanamura, Herminia Y. Silva, Rita Maria Rossi, Cristina Renata Nardoto Andrade Jr., Heitor Franco de Amato Neto, Vicente |
author_role |
author |
author2 |
Kanamura, Herminia Y. Silva, Rita Maria Rossi, Cristina Renata Nardoto Andrade Jr., Heitor Franco de Amato Neto, Vicente |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pinto, Pedro Luiz Silva Kanamura, Herminia Y. Silva, Rita Maria Rossi, Cristina Renata Nardoto Andrade Jr., Heitor Franco de Amato Neto, Vicente |
dc.subject.por.fl_str_mv |
Schistosomiasis DOT-ELISA Egg excretion Diagnosis |
topic |
Schistosomiasis DOT-ELISA Egg excretion Diagnosis |
description |
Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29245 |
url |
https://www.revistas.usp.br/rimtsp/article/view/29245 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/29245/31102 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 2 (1995); 109-115 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 2 (1995); 109-115 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 2 (1995); 109-115 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
||revimtsp@usp.br |
_version_ |
1798951641121030144 |