Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients

Detalhes bibliográficos
Autor(a) principal: Pinto, Pedro Luiz Silva
Data de Publicação: 1995
Outros Autores: Kanamura, Herminia Y., Silva, Rita Maria, Rossi, Cristina Renata Nardoto, Andrade Jr., Heitor Franco de, Amato Neto, Vicente
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/29245
Resumo: Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies.
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spelling Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes SchistosomiasisDOT-ELISAEgg excretionDiagnosis Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies. A esquistosomose humana, causada pelo Schistosoma mansoni, é altamente prevalente no Brasil e é usualmente diagnosticada através de exames de fezes, que são demorados. Os testes sorológicos nesta doença limitam-se aos estudos epidemiológicos, mostrando apenas o contato prévio com o parasita e não a infecção realmente ativa. Neste trabalho, nós padronizamos e comparamos um novo ensaio de Dot-ELISA, para detecção de IgM e IgG específicas contra antígenos de ovo e vermes de S. mansoni, comparando seus resultados com a técnica clássica de imunofluorescência (IFI), tanto para IgG ou IgM, utilizando antígenos similares, no estudo de uma população de 27 pacientes com quantificação de ovos nas fezes. A positividade do teste de Dot-ELISA foi de 96,3% para IgG e 88,9% para IgM, com antígeno de verme, e 92,6% para IgG e 90,9% para IgM com antígeno de ovo, que foi comparado com a IFI que apresentou resultados similares com antígeno de verme, 92,6% (IgG) e 96,3% (IgM) e menores com antígeno de ovo, 77,8% (IgG e IgM). Nosso grupo de pacientes claramente apresentava dois subgrupos, quanto à excreção de ovos nas fezes, com maior positividade dos testes sorológicos para o grupo composto por pacientes com maior excreção fecal de ovos. Quando comparamos a excreção quantitativa de ovos nas fezes com os títulos sorológicos, detectamos apenas correlação entre estes valores e o Dot-ELISA IgM (r=0,552 p=0.0127). Estes dados mostram que o teste de Dot-ELISA pode ser usado para a detecção de anticorpos específicos contra o S. mansoni em soros de pacientes suspeitos, para estudos epidemiológicos de prevalência; e, com maior purificação dos antígenos de ovos e amostragem ampliada, poderá tornar-se um teste útil para uma estimativa grosseira da carga parasitária em estudos epidemiológicos. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1995-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/29245Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 2 (1995); 109-115 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 2 (1995); 109-115 Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 2 (1995); 109-115 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/29245/31102Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessPinto, Pedro Luiz SilvaKanamura, Herminia Y.Silva, Rita MariaRossi, Cristina Renata NardotoAndrade Jr., Heitor Franco deAmato Neto, Vicente2012-07-02T01:39:50Zoai:revistas.usp.br:article/29245Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:59.712484Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
DOT-ELISA para a detecção de anticorpos IgG e IgM para antígenos de ovos e vermes de Schistosoma mansoni, em associação com excreção de ovos por pacientes
title Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
spellingShingle Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
Pinto, Pedro Luiz Silva
Schistosomiasis
DOT-ELISA
Egg excretion
Diagnosis
title_short Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
title_full Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
title_fullStr Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
title_full_unstemmed Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
title_sort Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients
author Pinto, Pedro Luiz Silva
author_facet Pinto, Pedro Luiz Silva
Kanamura, Herminia Y.
Silva, Rita Maria
Rossi, Cristina Renata Nardoto
Andrade Jr., Heitor Franco de
Amato Neto, Vicente
author_role author
author2 Kanamura, Herminia Y.
Silva, Rita Maria
Rossi, Cristina Renata Nardoto
Andrade Jr., Heitor Franco de
Amato Neto, Vicente
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto, Pedro Luiz Silva
Kanamura, Herminia Y.
Silva, Rita Maria
Rossi, Cristina Renata Nardoto
Andrade Jr., Heitor Franco de
Amato Neto, Vicente
dc.subject.por.fl_str_mv Schistosomiasis
DOT-ELISA
Egg excretion
Diagnosis
topic Schistosomiasis
DOT-ELISA
Egg excretion
Diagnosis
description Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg stool excretion. The positivity obtained for IgG Dot-ELISA was 96.3% and 88.9% for IgM Dot-ELISA with worm antigen and 92.6% and 90.9% with egg antigen. The IFI presented similar positivities using worm antigen, 92.6% (IgG) and 96.3% (IgM),and lower results with egg antigen, 77.8% (IgG and IgM). The patients studied were divided into two groups according to their egg excretion, with greater positivity of serological tests in higher egg excreters. When comparing the quantitative egg excretion and the serological titers of the patients, we detected a correlation only with IgM Dot-ELISA, with r=0.552 (p=0.0127). These data show that Dot-ELISA can be used for the detection of specific antibodies against S. mansoni in sera from suspected patients or in epidemiological studies and, with further purification of egg antigen and larger samples, IgM Dot-ELISA could be a possible tool for rough estimates of parasite burden in epidemiological studies.
publishDate 1995
dc.date.none.fl_str_mv 1995-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29245
url https://www.revistas.usp.br/rimtsp/article/view/29245
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/29245/31102
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 No. 2 (1995); 109-115
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 37 Núm. 2 (1995); 109-115
Revista do Instituto de Medicina Tropical de São Paulo; v. 37 n. 2 (1995); 109-115
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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