Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms

Detalhes bibliográficos
Autor(a) principal: David,Caroline Nespolo de
Data de Publicação: 2022
Outros Autores: Varela,Fernanda Hammes, Sartor,Ivaine Tais Sauthier, Polese-Bonatto,Márcia, Fernandes,Ingrid Rodrigues, Zavaglia,Gabriela Oliveira, Kern,Luciane Beatriz, Ferreira,Charles Francisco, Bastos,Gisele Alsina Nader, Pitrez,Paulo Márcio, Almeida,Walquiria Aparecida Ferreira de, Porto,Victor Bertollo Gomes, Zavascki,Alexandre Prehn, Stein,Renato Tetelbom, Scotta,Marcelo Comerlato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Cadernos de Saúde Pública
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-311X2022000105007
Resumo: Point-of-care serological tests for SARS-CoV-2 have been used for COVID-19 diagnosis. However, their accuracy over time regarding the onset of symptoms is not fully understood. We aimed to assess the accuracy of a point-of-care lateral flow immunoassay (LFI). Subjects, aged over 18 years, presenting clinical symptoms suggestive of acute SARS-CoV-2 infection were tested once by both nasopharyngeal and oropharyngeal RT-PCR and LFI. The accuracy of LFI was assessed in periodic intervals of three days in relation to the onset of symptoms. The optimal cut-off point was defined as the number of days required to achieve the best sensitivity and specificity. This cut-off point was also used to compare LFI accuracy according to participants’ status: outpatient or hospitalized. In total, 959 patients were included, 379 (39.52%) tested positive for SARS-CoV-2 with RT-PCR, and 272 (28.36%) tested positive with LFI. LFI best performance was achieved after 10 days of the onset of symptoms, with sensitivity and specificity of 84.9% (95%CI: 79.8-89.1) and 94.4% (95%CI: 91.0-96.8), respectively. Although the specificity was similar (94.6% vs. 88.9%, p = 0.051), the sensitivity was higher in hospitalized patients than in outpatients (91.7% vs. 82.1%, p = 0.032) after 10 days of the onset of symptoms. Best sensitivity of point-of-care LFI was found 10 days after the onset of symptoms which may limit its use in acute care. Specificity remained high regardless of the number of days since the onset of symptoms.
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spelling Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptomsSARS-CoV-2COVID-19 TestingPoint-of-Care TestingImmunoassaySensitivity and SpecificityPoint-of-care serological tests for SARS-CoV-2 have been used for COVID-19 diagnosis. However, their accuracy over time regarding the onset of symptoms is not fully understood. We aimed to assess the accuracy of a point-of-care lateral flow immunoassay (LFI). Subjects, aged over 18 years, presenting clinical symptoms suggestive of acute SARS-CoV-2 infection were tested once by both nasopharyngeal and oropharyngeal RT-PCR and LFI. The accuracy of LFI was assessed in periodic intervals of three days in relation to the onset of symptoms. The optimal cut-off point was defined as the number of days required to achieve the best sensitivity and specificity. This cut-off point was also used to compare LFI accuracy according to participants’ status: outpatient or hospitalized. In total, 959 patients were included, 379 (39.52%) tested positive for SARS-CoV-2 with RT-PCR, and 272 (28.36%) tested positive with LFI. LFI best performance was achieved after 10 days of the onset of symptoms, with sensitivity and specificity of 84.9% (95%CI: 79.8-89.1) and 94.4% (95%CI: 91.0-96.8), respectively. Although the specificity was similar (94.6% vs. 88.9%, p = 0.051), the sensitivity was higher in hospitalized patients than in outpatients (91.7% vs. 82.1%, p = 0.032) after 10 days of the onset of symptoms. Best sensitivity of point-of-care LFI was found 10 days after the onset of symptoms which may limit its use in acute care. Specificity remained high regardless of the number of days since the onset of symptoms.Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-311X2022000105007Cadernos de Saúde Pública v.38 n.1 2022reponame:Cadernos de Saúde Públicainstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZ10.1590/0102-311x00069921info:eu-repo/semantics/openAccessDavid,Caroline Nespolo deVarela,Fernanda HammesSartor,Ivaine Tais SauthierPolese-Bonatto,MárciaFernandes,Ingrid RodriguesZavaglia,Gabriela OliveiraKern,Luciane BeatrizFerreira,Charles FranciscoBastos,Gisele Alsina NaderPitrez,Paulo MárcioAlmeida,Walquiria Aparecida Ferreira dePorto,Victor Bertollo GomesZavascki,Alexandre PrehnStein,Renato TetelbomScotta,Marcelo Comerlatoeng2022-01-10T00:00:00Zoai:scielo:S0102-311X2022000105007Revistahttp://cadernos.ensp.fiocruz.br/csp/https://old.scielo.br/oai/scielo-oai.phpcadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br1678-44640102-311Xopendoar:2022-01-10T00:00Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
title Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
spellingShingle Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
David,Caroline Nespolo de
SARS-CoV-2
COVID-19 Testing
Point-of-Care Testing
Immunoassay
Sensitivity and Specificity
title_short Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
title_full Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
title_fullStr Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
title_full_unstemmed Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
title_sort Diagnostic accuracy of a SARS-CoV-2 rapid test and optimal time for seropositivity according to the onset of symptoms
author David,Caroline Nespolo de
author_facet David,Caroline Nespolo de
Varela,Fernanda Hammes
Sartor,Ivaine Tais Sauthier
Polese-Bonatto,Márcia
Fernandes,Ingrid Rodrigues
Zavaglia,Gabriela Oliveira
Kern,Luciane Beatriz
Ferreira,Charles Francisco
Bastos,Gisele Alsina Nader
Pitrez,Paulo Márcio
Almeida,Walquiria Aparecida Ferreira de
Porto,Victor Bertollo Gomes
Zavascki,Alexandre Prehn
Stein,Renato Tetelbom
Scotta,Marcelo Comerlato
author_role author
author2 Varela,Fernanda Hammes
Sartor,Ivaine Tais Sauthier
Polese-Bonatto,Márcia
Fernandes,Ingrid Rodrigues
Zavaglia,Gabriela Oliveira
Kern,Luciane Beatriz
Ferreira,Charles Francisco
Bastos,Gisele Alsina Nader
Pitrez,Paulo Márcio
Almeida,Walquiria Aparecida Ferreira de
Porto,Victor Bertollo Gomes
Zavascki,Alexandre Prehn
Stein,Renato Tetelbom
Scotta,Marcelo Comerlato
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv David,Caroline Nespolo de
Varela,Fernanda Hammes
Sartor,Ivaine Tais Sauthier
Polese-Bonatto,Márcia
Fernandes,Ingrid Rodrigues
Zavaglia,Gabriela Oliveira
Kern,Luciane Beatriz
Ferreira,Charles Francisco
Bastos,Gisele Alsina Nader
Pitrez,Paulo Márcio
Almeida,Walquiria Aparecida Ferreira de
Porto,Victor Bertollo Gomes
Zavascki,Alexandre Prehn
Stein,Renato Tetelbom
Scotta,Marcelo Comerlato
dc.subject.por.fl_str_mv SARS-CoV-2
COVID-19 Testing
Point-of-Care Testing
Immunoassay
Sensitivity and Specificity
topic SARS-CoV-2
COVID-19 Testing
Point-of-Care Testing
Immunoassay
Sensitivity and Specificity
description Point-of-care serological tests for SARS-CoV-2 have been used for COVID-19 diagnosis. However, their accuracy over time regarding the onset of symptoms is not fully understood. We aimed to assess the accuracy of a point-of-care lateral flow immunoassay (LFI). Subjects, aged over 18 years, presenting clinical symptoms suggestive of acute SARS-CoV-2 infection were tested once by both nasopharyngeal and oropharyngeal RT-PCR and LFI. The accuracy of LFI was assessed in periodic intervals of three days in relation to the onset of symptoms. The optimal cut-off point was defined as the number of days required to achieve the best sensitivity and specificity. This cut-off point was also used to compare LFI accuracy according to participants’ status: outpatient or hospitalized. In total, 959 patients were included, 379 (39.52%) tested positive for SARS-CoV-2 with RT-PCR, and 272 (28.36%) tested positive with LFI. LFI best performance was achieved after 10 days of the onset of symptoms, with sensitivity and specificity of 84.9% (95%CI: 79.8-89.1) and 94.4% (95%CI: 91.0-96.8), respectively. Although the specificity was similar (94.6% vs. 88.9%, p = 0.051), the sensitivity was higher in hospitalized patients than in outpatients (91.7% vs. 82.1%, p = 0.032) after 10 days of the onset of symptoms. Best sensitivity of point-of-care LFI was found 10 days after the onset of symptoms which may limit its use in acute care. Specificity remained high regardless of the number of days since the onset of symptoms.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-311X2022000105007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-311X2022000105007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0102-311x00069921
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz
publisher.none.fl_str_mv Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz
dc.source.none.fl_str_mv Cadernos de Saúde Pública v.38 n.1 2022
reponame:Cadernos de Saúde Pública
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Cadernos de Saúde Pública
collection Cadernos de Saúde Pública
repository.name.fl_str_mv Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv cadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br
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