Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus

Detalhes bibliográficos
Autor(a) principal: Melo, André L. A.
Data de Publicação: 2009
Outros Autores: Soccol, Carlos R., Thomaz-Soccol, Vanete, Nogueira Jr.S, Miodeli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Cadernos de Saúde Pública
Texto Completo: https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135
Resumo: Bioinsecticides are shown to be useful in control programs to prevent several diseases, based on their specificity and efficiency against insect vectors. In the current study a bioinsecticide based on Bacillus sphaericus was produced using a white soybean culture medium and applied to larvae of Culex quinquefasciatus, the susceptible species, and Aedes aegypti, the refractory species used as the negative control. Efficacy was compared with that of the product fermented with the Luria Bertani (LB) reference medium. The experiments showed that C. quinquefasciatus was highly susceptible to the product prepared with white soybean meal, reaching 100% larval mortality even at 10mg/L, while A. aegypti failed to reach 70% mortality at a concentration of 1g/L. By comparison with the reference medium, the proposed culture medium showed high larvicidal power, reaching a LD90 of 2.26mg/L, while 4.37mg/L was needed for the LB medium to achieve the same mortality rate. Cost comparison between the formulations favored the use of the bioinsecticide produced with white soybean meal. After factoring in the LD90 value, the cost ratio favored the new raw material by nearly 1:220.
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spelling Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatusInsecticidesVector ControlSoybeansBioinsecticides are shown to be useful in control programs to prevent several diseases, based on their specificity and efficiency against insect vectors. In the current study a bioinsecticide based on Bacillus sphaericus was produced using a white soybean culture medium and applied to larvae of Culex quinquefasciatus, the susceptible species, and Aedes aegypti, the refractory species used as the negative control. Efficacy was compared with that of the product fermented with the Luria Bertani (LB) reference medium. The experiments showed that C. quinquefasciatus was highly susceptible to the product prepared with white soybean meal, reaching 100% larval mortality even at 10mg/L, while A. aegypti failed to reach 70% mortality at a concentration of 1g/L. By comparison with the reference medium, the proposed culture medium showed high larvicidal power, reaching a LD90 of 2.26mg/L, while 4.37mg/L was needed for the LB medium to achieve the same mortality rate. Cost comparison between the formulations favored the use of the bioinsecticide produced with white soybean meal. After factoring in the LD90 value, the cost ratio favored the new raw material by nearly 1:220.A utilização de bioinseticidas tem se mostrado útil aos programas de prevenção de diversas enfermidades devido a sua especificidade e eficiência contra insetos vetores. No presente trabalho, o bioinseticida de Bacillus sphaericus foi produzido com um meio de cultivo composto de farelo branco de soja e aplicado em larvas de Culex quinquefasciatus, espécie susce-tível, e Aedes aegypti, espécie refratária, usada como controle negativo. O desempenho foi comparado com o do produto fermentado com o meio referência Luria Bertani (LB). Os experimentos constataram que C. quinquefasciatus apresentou uma alta suscetibilidade ao produto produzido com farelo branco de soja, alcançando mortalidade de 100% mesmo na diluição de 10mg/L, enquanto A. aegypti não atingiu 70% na concentração de 1g/L. Quando comparado com o meio referência, a formulação proposta proporcionou um alto poder larvicida, alcançando uma DL90 de 2,26mg/L, ao passo que LB precisou de 4,37mg/L para a mesma mortalidade. A comparação do custo das formulações favoreceu o bioinseticida produzido com o meio farelo branco de soja. Considerando os valores de DL90, a relação do custo das matérias-primas ficou próxima de 1:220.Reports in Public HealthCadernos de Saúde Pública2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlapplication/pdfhttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135Reports in Public Health; Vol. 25 No. 3 (2009): MarchCadernos de Saúde Pública; v. 25 n. 3 (2009): Março1678-44640102-311Xreponame:Cadernos de Saúde Públicainstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135/8407https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135/8408Melo, André L. A.Soccol, Carlos R.Thomaz-Soccol, VaneteNogueira Jr.S, Miodeliinfo:eu-repo/semantics/openAccess2024-03-06T15:28:01Zoai:ojs.teste-cadernos.ensp.fiocruz.br:article/4135Revistahttps://cadernos.ensp.fiocruz.br/ojs/index.php/csphttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/oaicadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br1678-44640102-311Xopendoar:2024-03-06T13:04:46.654638Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)true
dc.title.none.fl_str_mv Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
title Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
spellingShingle Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
Melo, André L. A.
Insecticides
Vector Control
Soybeans
title_short Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
title_full Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
title_fullStr Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
title_full_unstemmed Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
title_sort Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus
author Melo, André L. A.
author_facet Melo, André L. A.
Soccol, Carlos R.
Thomaz-Soccol, Vanete
Nogueira Jr.S, Miodeli
author_role author
author2 Soccol, Carlos R.
Thomaz-Soccol, Vanete
Nogueira Jr.S, Miodeli
author2_role author
author
author
dc.contributor.author.fl_str_mv Melo, André L. A.
Soccol, Carlos R.
Thomaz-Soccol, Vanete
Nogueira Jr.S, Miodeli
dc.subject.por.fl_str_mv Insecticides
Vector Control
Soybeans
topic Insecticides
Vector Control
Soybeans
description Bioinsecticides are shown to be useful in control programs to prevent several diseases, based on their specificity and efficiency against insect vectors. In the current study a bioinsecticide based on Bacillus sphaericus was produced using a white soybean culture medium and applied to larvae of Culex quinquefasciatus, the susceptible species, and Aedes aegypti, the refractory species used as the negative control. Efficacy was compared with that of the product fermented with the Luria Bertani (LB) reference medium. The experiments showed that C. quinquefasciatus was highly susceptible to the product prepared with white soybean meal, reaching 100% larval mortality even at 10mg/L, while A. aegypti failed to reach 70% mortality at a concentration of 1g/L. By comparison with the reference medium, the proposed culture medium showed high larvicidal power, reaching a LD90 of 2.26mg/L, while 4.37mg/L was needed for the LB medium to achieve the same mortality rate. Cost comparison between the formulations favored the use of the bioinsecticide produced with white soybean meal. After factoring in the LD90 value, the cost ratio favored the new raw material by nearly 1:220.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135
url https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135/8407
https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/4135/8408
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
application/pdf
dc.publisher.none.fl_str_mv Reports in Public Health
Cadernos de Saúde Pública
publisher.none.fl_str_mv Reports in Public Health
Cadernos de Saúde Pública
dc.source.none.fl_str_mv Reports in Public Health; Vol. 25 No. 3 (2009): March
Cadernos de Saúde Pública; v. 25 n. 3 (2009): Março
1678-4464
0102-311X
reponame:Cadernos de Saúde Pública
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Cadernos de Saúde Pública
collection Cadernos de Saúde Pública
repository.name.fl_str_mv Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv cadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br
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