Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Vigilância Sanitária em Debate |
Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387 |
Resumo: | Introduction: The microbiological quality of hemodialysis treated water is directly related to the occurrence of infections and pyrogenic reactions in patients. Objective: Determine the minimum incubation time and evaluate the alternative microbiological method performance for heterotrophic bacteria count in hemodialysis water through the fluorescence microbial detection technique. Method: The analyses were conducted by concentration levels of 2,5 x 10-1 to 1,0 x 102 CFU/plate for Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli and Staphylococcus aureus. The tests were performed simultaneously by the alternative and the traditional methods, using culture medium R2A and incubation temperature of 24.0°C ± 4.0°C. The incubation times were 40 h and 120 h, respectively. Fourteen hemodialysis water samples were analyzed to assess the equivalence between the methods evaluated. Results: The results demonstrated that the alternative method allows quantification of heterotrophic bacteria after 40 h of incubation, with accuracy, precision, specificity and linearity for the range of 5 to 100 CFU/plate. The detection limit of the alternative method is 1 CFU/plate. Conclusions: It was possible to conclude that the alternative method has equivalent results to the traditional method, since the confidence interval of the alternative method was entirely within the equivalence range. Therefore, the microbial detection technique by fluorescence showed a viable option for the implementation of a rapid microbiological method for the heterotrophic bacteria count in samples of treated water for hemodialysis. |
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Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis waterAvaliação da técnica de detecção por fluorescência como alternativa para contagem de bactérias heterotróficas em água para hemodiáliseHemodialysisTreated WaterHeterotrophic BacteriaFluorescenceHemodiáliseÁgua TratadaBactérias HeterotróficasFluorescênciaIntroduction: The microbiological quality of hemodialysis treated water is directly related to the occurrence of infections and pyrogenic reactions in patients. Objective: Determine the minimum incubation time and evaluate the alternative microbiological method performance for heterotrophic bacteria count in hemodialysis water through the fluorescence microbial detection technique. Method: The analyses were conducted by concentration levels of 2,5 x 10-1 to 1,0 x 102 CFU/plate for Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli and Staphylococcus aureus. The tests were performed simultaneously by the alternative and the traditional methods, using culture medium R2A and incubation temperature of 24.0°C ± 4.0°C. The incubation times were 40 h and 120 h, respectively. Fourteen hemodialysis water samples were analyzed to assess the equivalence between the methods evaluated. Results: The results demonstrated that the alternative method allows quantification of heterotrophic bacteria after 40 h of incubation, with accuracy, precision, specificity and linearity for the range of 5 to 100 CFU/plate. The detection limit of the alternative method is 1 CFU/plate. Conclusions: It was possible to conclude that the alternative method has equivalent results to the traditional method, since the confidence interval of the alternative method was entirely within the equivalence range. Therefore, the microbial detection technique by fluorescence showed a viable option for the implementation of a rapid microbiological method for the heterotrophic bacteria count in samples of treated water for hemodialysis.Introdução: A qualidade microbiológica da água tratada para hemodiálise está diretamente relacionada à ocorrência de infecções e de reações pirogênicas nos pacientes. Objetivo: Determinar o tempo de incubação mínimo e avaliar o desempenho do método microbiológico alternativo para a contagem de bactérias heterotróficas em água de hemodiálise por meio da técnica de detecção microbiana por fluorescência. Método: As análises foram conduzidas com níveis de concentração entre 2,5 x 10-1 e 1,0 x 102 UFC/placa para Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli e Staphylococcus aureus. Os testes foram realizados simultaneamente pelos métodos alternativo e tradicional, utilizando o meio de cultura R2A e temperatura de incubação de 24,0°C ± 4,0°C. Os tempos de incubação empregados foram os de 40 h e 120 h, respectivamente. Quatorze amostras de água para hemodiálise foram analisadas para avaliação da equivalência entre os métodos avaliados. Resultados: Os resultados demonstraram que o método alternativo permite a quantificação de bactérias heterotróficas após 40 h de incubação, com precisão, exatidão, especificidade e linearidade para a faixa de 5 a 100 UFC/placa. O limite de detecção do método alternativo é 1 UFC/placa. Conclusões: O método alternativo possui resultados equivalentes ao método tradicional, uma vez que o intervalo de confiança do método alternativo obtido esteve compreendido inteiramente dentro da faixa de equivalência. Portanto, a técnica de detecção microbiana por fluorescência mostrou ser uma opção viável para a implementação de um método microbiológico rápido para a contagem de bactérias heterotróficas em amostras de água tratada para hemodiálise.Instituto Nacional de Controle de Qualidade em Saúde2020-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/138710.22239/2317-269X.01387Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 2 (2020): May; 94-105Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 2 (2020): Puede; 94-105Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 2 (2020): Maio; 94-1052317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387/1220https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387/1221Copyright (c) 2020 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by/3.0/deed.pt_BRinfo:eu-repo/semantics/openAccessHilinski, Ellen GameiroBugno, AdrianaSilva, Fernando Pontes de Lima eAlmodovar, Adriana Aparecida BuzzoPinto, Terezinha de Jesus Andreoli2023-06-27T15:12:27Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1387Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:12:27Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water Avaliação da técnica de detecção por fluorescência como alternativa para contagem de bactérias heterotróficas em água para hemodiálise |
title |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water |
spellingShingle |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water Hilinski, Ellen Gameiro Hemodialysis Treated Water Heterotrophic Bacteria Fluorescence Hemodiálise Água Tratada Bactérias Heterotróficas Fluorescência |
title_short |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water |
title_full |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water |
title_fullStr |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water |
title_full_unstemmed |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water |
title_sort |
Evaluation of fluorescence detection technique as an alternative for heterotrophic bacteria count in hemodialysis water |
author |
Hilinski, Ellen Gameiro |
author_facet |
Hilinski, Ellen Gameiro Bugno, Adriana Silva, Fernando Pontes de Lima e Almodovar, Adriana Aparecida Buzzo Pinto, Terezinha de Jesus Andreoli |
author_role |
author |
author2 |
Bugno, Adriana Silva, Fernando Pontes de Lima e Almodovar, Adriana Aparecida Buzzo Pinto, Terezinha de Jesus Andreoli |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hilinski, Ellen Gameiro Bugno, Adriana Silva, Fernando Pontes de Lima e Almodovar, Adriana Aparecida Buzzo Pinto, Terezinha de Jesus Andreoli |
dc.subject.por.fl_str_mv |
Hemodialysis Treated Water Heterotrophic Bacteria Fluorescence Hemodiálise Água Tratada Bactérias Heterotróficas Fluorescência |
topic |
Hemodialysis Treated Water Heterotrophic Bacteria Fluorescence Hemodiálise Água Tratada Bactérias Heterotróficas Fluorescência |
description |
Introduction: The microbiological quality of hemodialysis treated water is directly related to the occurrence of infections and pyrogenic reactions in patients. Objective: Determine the minimum incubation time and evaluate the alternative microbiological method performance for heterotrophic bacteria count in hemodialysis water through the fluorescence microbial detection technique. Method: The analyses were conducted by concentration levels of 2,5 x 10-1 to 1,0 x 102 CFU/plate for Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli and Staphylococcus aureus. The tests were performed simultaneously by the alternative and the traditional methods, using culture medium R2A and incubation temperature of 24.0°C ± 4.0°C. The incubation times were 40 h and 120 h, respectively. Fourteen hemodialysis water samples were analyzed to assess the equivalence between the methods evaluated. Results: The results demonstrated that the alternative method allows quantification of heterotrophic bacteria after 40 h of incubation, with accuracy, precision, specificity and linearity for the range of 5 to 100 CFU/plate. The detection limit of the alternative method is 1 CFU/plate. Conclusions: It was possible to conclude that the alternative method has equivalent results to the traditional method, since the confidence interval of the alternative method was entirely within the equivalence range. Therefore, the microbial detection technique by fluorescence showed a viable option for the implementation of a rapid microbiological method for the heterotrophic bacteria count in samples of treated water for hemodialysis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387 10.22239/2317-269X.01387 |
url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387 |
identifier_str_mv |
10.22239/2317-269X.01387 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387/1220 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1387/1221 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/3.0/deed.pt_BR info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/3.0/deed.pt_BR |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 2 (2020): May; 94-105 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 2 (2020): Puede; 94-105 Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 2 (2020): Maio; 94-105 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Vigilância Sanitária em Debate |
collection |
Vigilância Sanitária em Debate |
repository.name.fl_str_mv |
Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
_version_ |
1797042045784686592 |