Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Vigilância Sanitária em Debate |
Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296 |
Resumo: | Introduction: Antimicrobial resistance is a public health problem. Foods may be carriers of antimicrobial resistant bacteria and resistance genes for humans. Objective: To isolate, identify, and evaluate the antimicrobial susceptibility and identification of resistance genes and integrons in Escherichia coli strains isolated from Minas Frescal cheese. Method: The presence of E. coli in thirty samples of Minas Frescal cheese was evaluated through the 3M™ Petrifilm plates. Thirty . coli isolates were evaluated through the Disk Diffusion Susceptibility Test for 17 antimicrobial agents. Through PCR and Multiplex PCR the isolates were examined for the presence of 24 antimicrobial resistance genes. Genes and variable regions of class 1, 2 and 3 integrons were also evaluated. Results: Phenotypic resistance to one or more classes of antimicrobial agents were found in 50% of E. coli isolates. One hundred percent of the isolates showed PCR amplification for the blaTEM gene and there was also amplification for blaSHV and tetB genes. In addition, 46.6% of the phenotypically resistant isolates amplified to one or more classes of integrons. This is one of the first studies to identify these genes in Minas Frescal cheese in Brazil. Conclusions: Cheese can be a source of multiresistant bacteria and those can disseminate their resistance genes to other bacteria being in the food and human gastrointestinal tract, emphasizing the importance of Good Manufacturing Practices and greater supervision on products on sale. |
id |
FIOCRUZ-9_33f7009f778e896a82f64746a365e5b9 |
---|---|
oai_identifier_str |
oai:ojs.visaemdebate.incqs.fiocruz.br:article/1296 |
network_acronym_str |
FIOCRUZ-9 |
network_name_str |
Vigilância Sanitária em Debate |
repository_id_str |
|
spelling |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profileResistência antimicrobiana em cepas de Escherichia coli isoladas de queijo Minas Frescal no município do Rio de Janeiro – Perfil fenotípico e genotípicoResistência antimicrobianaE. coliIntegronQueijo Minas FrescalAntimicrobial resistanceE.coliIntegronMinas Frescal CheeseIntroduction: Antimicrobial resistance is a public health problem. Foods may be carriers of antimicrobial resistant bacteria and resistance genes for humans. Objective: To isolate, identify, and evaluate the antimicrobial susceptibility and identification of resistance genes and integrons in Escherichia coli strains isolated from Minas Frescal cheese. Method: The presence of E. coli in thirty samples of Minas Frescal cheese was evaluated through the 3M™ Petrifilm plates. Thirty . coli isolates were evaluated through the Disk Diffusion Susceptibility Test for 17 antimicrobial agents. Through PCR and Multiplex PCR the isolates were examined for the presence of 24 antimicrobial resistance genes. Genes and variable regions of class 1, 2 and 3 integrons were also evaluated. Results: Phenotypic resistance to one or more classes of antimicrobial agents were found in 50% of E. coli isolates. One hundred percent of the isolates showed PCR amplification for the blaTEM gene and there was also amplification for blaSHV and tetB genes. In addition, 46.6% of the phenotypically resistant isolates amplified to one or more classes of integrons. This is one of the first studies to identify these genes in Minas Frescal cheese in Brazil. Conclusions: Cheese can be a source of multiresistant bacteria and those can disseminate their resistance genes to other bacteria being in the food and human gastrointestinal tract, emphasizing the importance of Good Manufacturing Practices and greater supervision on products on sale.Introdução: A resistência antimicrobiana é um problema de saúde pública. Os alimentos podem ser veículos de bactérias resistentes a antimicrobianos e genes de resistência para os seres humanos. Objetivo: Isolamento, identificação, avaliação da susceptibilidade antimicrobiana e identificação de genes de resistência e integrons em Escherichia coli isoladas de queijo Minas Frescal. Método: Avaliação da presença de E. coli em trinta amostras de queijo Minas Frescal por meio de Placas Petrifilm 3M™. Trinta isolados de E. coli foram avaliados através do teste de sensibilidade por disco difusão para 17 agentes antimicrobianos. Isolados fenotipicamente resistentes foram examinados por meio de PCR e Multiplex PCR para 24 genes de resistência. Genes e regiões variáveis dos integrons de classe 1, 2 e 3 também foram avaliados. Resultados: Em 50% dos isolados verificou-se resistência fenotípica a uma ou mais classes de antimicrobianos. Cem por cento dos isolados apresentaram o gene blaTEM e houve amplificação dos genes blaSHV e tetB. Além disso, 46,6% dos isolados fenotipicamente resistentes amplificaram para uma ou mais classes de integrons. Este é um dos primeiros estudos a identificar esses genes em queijo Minas Frescal no Brasil. Conclusões: O queijo pode ser uma fonte de bactérias multirresistentes e estas podem disseminar seus genes de resistência a bactérias presentes no alimento e no trato gastrointestinal humano, demonstrando a importância das Boas Práticas de Fabricação e a necessidade de maior fiscalização dos produtos colocados à venda.Instituto Nacional de Controle de Qualidade em Saúde2019-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/129610.22239/2317-269X.01296Health Surveillance under Debate: Society, Science & Technology ; Vol. 7 No. 3 (2019): August; 86-91Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 7 Núm. 3 (2019): Agosto; 86-91Vigil Sanit Debate, Rio de Janeiro; v. 7 n. 3 (2019): Agosto; 86-912317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296/1040https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296/1173Copyright (c) 2019 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessPires, Bruna Amatto DuarteOliveira, Juliana Wolff Salles deSilva, Cristiane RodriguesAbrantes, Shirley de Mello PereiraMarin, Victor Augustus2023-06-27T15:11:24Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1296Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:11:24Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile Resistência antimicrobiana em cepas de Escherichia coli isoladas de queijo Minas Frescal no município do Rio de Janeiro – Perfil fenotípico e genotípico |
title |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile |
spellingShingle |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile Pires, Bruna Amatto Duarte Resistência antimicrobiana E. coli Integron Queijo Minas Frescal Antimicrobial resistance E.coli Integron Minas Frescal Cheese |
title_short |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile |
title_full |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile |
title_fullStr |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile |
title_full_unstemmed |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile |
title_sort |
Antimicrobial resistance of Escherichia coli isolated from Minas Frescal Cheese in the city of Rio de Janeiro – Phenotypic and genotypic profile |
author |
Pires, Bruna Amatto Duarte |
author_facet |
Pires, Bruna Amatto Duarte Oliveira, Juliana Wolff Salles de Silva, Cristiane Rodrigues Abrantes, Shirley de Mello Pereira Marin, Victor Augustus |
author_role |
author |
author2 |
Oliveira, Juliana Wolff Salles de Silva, Cristiane Rodrigues Abrantes, Shirley de Mello Pereira Marin, Victor Augustus |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pires, Bruna Amatto Duarte Oliveira, Juliana Wolff Salles de Silva, Cristiane Rodrigues Abrantes, Shirley de Mello Pereira Marin, Victor Augustus |
dc.subject.por.fl_str_mv |
Resistência antimicrobiana E. coli Integron Queijo Minas Frescal Antimicrobial resistance E.coli Integron Minas Frescal Cheese |
topic |
Resistência antimicrobiana E. coli Integron Queijo Minas Frescal Antimicrobial resistance E.coli Integron Minas Frescal Cheese |
description |
Introduction: Antimicrobial resistance is a public health problem. Foods may be carriers of antimicrobial resistant bacteria and resistance genes for humans. Objective: To isolate, identify, and evaluate the antimicrobial susceptibility and identification of resistance genes and integrons in Escherichia coli strains isolated from Minas Frescal cheese. Method: The presence of E. coli in thirty samples of Minas Frescal cheese was evaluated through the 3M™ Petrifilm plates. Thirty . coli isolates were evaluated through the Disk Diffusion Susceptibility Test for 17 antimicrobial agents. Through PCR and Multiplex PCR the isolates were examined for the presence of 24 antimicrobial resistance genes. Genes and variable regions of class 1, 2 and 3 integrons were also evaluated. Results: Phenotypic resistance to one or more classes of antimicrobial agents were found in 50% of E. coli isolates. One hundred percent of the isolates showed PCR amplification for the blaTEM gene and there was also amplification for blaSHV and tetB genes. In addition, 46.6% of the phenotypically resistant isolates amplified to one or more classes of integrons. This is one of the first studies to identify these genes in Minas Frescal cheese in Brazil. Conclusions: Cheese can be a source of multiresistant bacteria and those can disseminate their resistance genes to other bacteria being in the food and human gastrointestinal tract, emphasizing the importance of Good Manufacturing Practices and greater supervision on products on sale. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296 10.22239/2317-269X.01296 |
url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296 |
identifier_str_mv |
10.22239/2317-269X.01296 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296/1040 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1296/1173 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 7 No. 3 (2019): August; 86-91 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 7 Núm. 3 (2019): Agosto; 86-91 Vigil Sanit Debate, Rio de Janeiro; v. 7 n. 3 (2019): Agosto; 86-91 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Vigilância Sanitária em Debate |
collection |
Vigilância Sanitária em Debate |
repository.name.fl_str_mv |
Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
_version_ |
1797042045371547648 |