Development of proficiency test items for Salmonella spp. research in chocolate matrix
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por eng |
| Título da fonte: | Vigilância Sanitária em Debate |
| Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838 |
Resumo: | The aim of this study was to develop lyophilized test items (TI) containing Salmonella spp., in chocolate matrix to be used in proficiency testing programs (PTP). Microbial analysis was conducted on samples of granulated chocolate to verify that the sample was free of the target microorganisms. Homogeneity and stability studies in long and short term were carried out to monitor TI quality; the presence of vacuum in the samples was also verified, to ensure the efficiency of the lyophilization process. The results of the microbial testing indicated no contamination by Salmonella spp.; thus, the sample was appropriate to be used as matrix. The lyophilization technique, using trehalose as cryoprotectant, has proven to be effective for desiccation of TI produced. The Salmonella batch proved to be sufficiently homogeneous, because the microorganism was present in all analyzed flasks. The batch was held stable at -20°C (five weeks) and -70°C (26 weeks). As for the transportation stability, the batch was considered stable at 4°C (in four days). The TI produced batch in this study showed a quality level that makes it suitable to be used in PTP, to contribute to the increasing reliability of the test results from laboratories and to provide subsidies for identification of problems and troubleshooting. |
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Development of proficiency test items for Salmonella spp. research in chocolate matrixDesenvolvimento de itens de ensaio de proficiência para pesquisa de Salmonella spp. em matriz chocolateItem de EnsaioEnsaio de ProficiênciaChocolateSalmonella sppVigilância SanitáriaTest ItemProficiency Testing ProgramsChocolateSalmonella sppSanitary SurveillanceThe aim of this study was to develop lyophilized test items (TI) containing Salmonella spp., in chocolate matrix to be used in proficiency testing programs (PTP). Microbial analysis was conducted on samples of granulated chocolate to verify that the sample was free of the target microorganisms. Homogeneity and stability studies in long and short term were carried out to monitor TI quality; the presence of vacuum in the samples was also verified, to ensure the efficiency of the lyophilization process. The results of the microbial testing indicated no contamination by Salmonella spp.; thus, the sample was appropriate to be used as matrix. The lyophilization technique, using trehalose as cryoprotectant, has proven to be effective for desiccation of TI produced. The Salmonella batch proved to be sufficiently homogeneous, because the microorganism was present in all analyzed flasks. The batch was held stable at -20°C (five weeks) and -70°C (26 weeks). As for the transportation stability, the batch was considered stable at 4°C (in four days). The TI produced batch in this study showed a quality level that makes it suitable to be used in PTP, to contribute to the increasing reliability of the test results from laboratories and to provide subsidies for identification of problems and troubleshooting.O objetivo desse estudo foi desenvolver itens de ensaio (IE) liofilizados contendo Salmonella spp., em matriz chocolate, para utilização em ensaio de proficiência (EP). Foi realizada a análise microbiológica de uma amostra de chocolate granulado para verificar se estava livre do micro-organismo alvo. Para monitoramento da qualidade dos IE, realizou-se estudos de homogeneidade e estabilidade em longo e curto prazo, bem como verificou-se a presença de vácuo nas amostras garantindo a eficiência do processo de liofilização. A análise microbiológica do chocolate indicou ausência de contaminação por Salmonella spp., estando apto para ser utilizado como matriz. A técnica de liofilização, com uso de trealose como crioprotetor, se mostrou eficaz para dessecação dos IE produzidos. O lote produzido se apresentou suficientemente homogêneo, pois o micro-organismo estava presente em todos os frascos analisados. O lote se apresentou estável à temperatura de -20ºC (em cinco semanas) e -70ºC (em 26 semanas); na estabilidade de transporte, foi considerado estável a 4ºC (em quatro dias). O lote de IE produzido nesse estudo apresentou qualidade que o torna apto para uso em EP, o que visou contribuir para o aumento da confiabilidade dos resultados das análises dos laboratórios e propiciar subsídios para a identificação e solução de problemas.Instituto Nacional de Controle de Qualidade em Saúde2017-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/83810.22239/2317-269X.00838Health Surveillance under Debate: Society, Science & Technology ; Vol. 5 No. 2 (2017): May; 106-112Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 5 Núm. 2 (2017): Puede; 106-112Vigil Sanit Debate, Rio de Janeiro; v. 5 n. 2 (2017): Maio; 106-1122317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838/380https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838/520Copyright (c) 2017 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessda Silva, Maria Luiza CabralBrandão, Marcelo Luiz LimaRosas, Carla de OliveiraMedeiros, Valéria de Melloda Silva, Cátia CardosoTavares, Rodrigo Domingos OveraLopes, Silvia Maria dos ReisCardarelli-Leite, Paola2023-06-27T15:19:44Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/838Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:19:44Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.none.fl_str_mv |
Development of proficiency test items for Salmonella spp. research in chocolate matrix Desenvolvimento de itens de ensaio de proficiência para pesquisa de Salmonella spp. em matriz chocolate |
| title |
Development of proficiency test items for Salmonella spp. research in chocolate matrix |
| spellingShingle |
Development of proficiency test items for Salmonella spp. research in chocolate matrix da Silva, Maria Luiza Cabral Item de Ensaio Ensaio de Proficiência Chocolate Salmonella spp Vigilância Sanitária Test Item Proficiency Testing Programs Chocolate Salmonella spp Sanitary Surveillance |
| title_short |
Development of proficiency test items for Salmonella spp. research in chocolate matrix |
| title_full |
Development of proficiency test items for Salmonella spp. research in chocolate matrix |
| title_fullStr |
Development of proficiency test items for Salmonella spp. research in chocolate matrix |
| title_full_unstemmed |
Development of proficiency test items for Salmonella spp. research in chocolate matrix |
| title_sort |
Development of proficiency test items for Salmonella spp. research in chocolate matrix |
| author |
da Silva, Maria Luiza Cabral |
| author_facet |
da Silva, Maria Luiza Cabral Brandão, Marcelo Luiz Lima Rosas, Carla de Oliveira Medeiros, Valéria de Mello da Silva, Cátia Cardoso Tavares, Rodrigo Domingos Overa Lopes, Silvia Maria dos Reis Cardarelli-Leite, Paola |
| author_role |
author |
| author2 |
Brandão, Marcelo Luiz Lima Rosas, Carla de Oliveira Medeiros, Valéria de Mello da Silva, Cátia Cardoso Tavares, Rodrigo Domingos Overa Lopes, Silvia Maria dos Reis Cardarelli-Leite, Paola |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
da Silva, Maria Luiza Cabral Brandão, Marcelo Luiz Lima Rosas, Carla de Oliveira Medeiros, Valéria de Mello da Silva, Cátia Cardoso Tavares, Rodrigo Domingos Overa Lopes, Silvia Maria dos Reis Cardarelli-Leite, Paola |
| dc.subject.por.fl_str_mv |
Item de Ensaio Ensaio de Proficiência Chocolate Salmonella spp Vigilância Sanitária Test Item Proficiency Testing Programs Chocolate Salmonella spp Sanitary Surveillance |
| topic |
Item de Ensaio Ensaio de Proficiência Chocolate Salmonella spp Vigilância Sanitária Test Item Proficiency Testing Programs Chocolate Salmonella spp Sanitary Surveillance |
| description |
The aim of this study was to develop lyophilized test items (TI) containing Salmonella spp., in chocolate matrix to be used in proficiency testing programs (PTP). Microbial analysis was conducted on samples of granulated chocolate to verify that the sample was free of the target microorganisms. Homogeneity and stability studies in long and short term were carried out to monitor TI quality; the presence of vacuum in the samples was also verified, to ensure the efficiency of the lyophilization process. The results of the microbial testing indicated no contamination by Salmonella spp.; thus, the sample was appropriate to be used as matrix. The lyophilization technique, using trehalose as cryoprotectant, has proven to be effective for desiccation of TI produced. The Salmonella batch proved to be sufficiently homogeneous, because the microorganism was present in all analyzed flasks. The batch was held stable at -20°C (five weeks) and -70°C (26 weeks). As for the transportation stability, the batch was considered stable at 4°C (in four days). The TI produced batch in this study showed a quality level that makes it suitable to be used in PTP, to contribute to the increasing reliability of the test results from laboratories and to provide subsidies for identification of problems and troubleshooting. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-05-31 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838 10.22239/2317-269X.00838 |
| url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838 |
| identifier_str_mv |
10.22239/2317-269X.00838 |
| dc.language.iso.fl_str_mv |
por eng |
| language |
por eng |
| dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838/380 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/838/520 |
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https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-nd/4.0 |
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openAccess |
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application/pdf application/pdf |
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Instituto Nacional de Controle de Qualidade em Saúde |
| publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
| dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 5 No. 2 (2017): May; 106-112 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 5 Núm. 2 (2017): Puede; 106-112 Vigil Sanit Debate, Rio de Janeiro; v. 5 n. 2 (2017): Maio; 106-112 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Vigilância Sanitária em Debate |
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Vigilância Sanitária em Debate |
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Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
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incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
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1797042044752887808 |