Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Detalhes bibliográficos
Autor(a) principal: MELO JÚNIOR,Paulo Maurício Reis de
Data de Publicação: 2015
Outros Autores: SOBRAL,Ana Paula Veras, SAMPAIO,Gerhilde Callou, PINTO,Isabel Maria de Araújo, SHINOHARA,Neide Kazue Sakugawa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: RGO - Revista Gaúcha de Odontologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200181
Resumo: OBJECTIVE: The objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. METHODS: Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. RESULTS: The diameters obtained to MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) were expressed in millimeters and subsequently submitted to Tukey test (p<0.05). CONCLUSION: According to the results obtained, it was concluded that the MTA and Portland cement showed to have similar antimicrobial properties, however these were not effective against the Enterococcus faecalis.
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spelling Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cementBactériaCimentos dentáriosProducts with antimicrobial action OBJECTIVE: The objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. METHODS: Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. RESULTS: The diameters obtained to MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) were expressed in millimeters and subsequently submitted to Tukey test (p<0.05). CONCLUSION: According to the results obtained, it was concluded that the MTA and Portland cement showed to have similar antimicrobial properties, however these were not effective against the Enterococcus faecalis.Faculdade São Leopoldo Mandic2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200181RGO - Revista Gaúcha de Odontologia v.63 n.2 2015reponame:RGO - Revista Gaúcha de Odontologia (Online)instname:Faculdade São Leopoldo Mandic (FSLM)instacron:FSLM10.1590/1981-863720150002000072733info:eu-repo/semantics/openAccessMELO JÚNIOR,Paulo Maurício Reis deSOBRAL,Ana Paula VerasSAMPAIO,Gerhilde CallouPINTO,Isabel Maria de AraújoSHINOHARA,Neide Kazue Sakugawaeng2019-08-06T00:00:00Zoai:scielo:S1981-86372015000200181Revistahttp://revodonto.bvsalud.org/scielo.php?script=sci_serial&pid=1981-8637&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||contato@revistargo.com.br1981-86370103-6971opendoar:2019-08-06T00:00RGO - Revista Gaúcha de Odontologia (Online) - Faculdade São Leopoldo Mandic (FSLM)false
dc.title.none.fl_str_mv Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
title Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
spellingShingle Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
MELO JÚNIOR,Paulo Maurício Reis de
Bactéria
Cimentos dentários
Products with antimicrobial action
title_short Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
title_full Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
title_fullStr Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
title_full_unstemmed Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
title_sort Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement
author MELO JÚNIOR,Paulo Maurício Reis de
author_facet MELO JÚNIOR,Paulo Maurício Reis de
SOBRAL,Ana Paula Veras
SAMPAIO,Gerhilde Callou
PINTO,Isabel Maria de Araújo
SHINOHARA,Neide Kazue Sakugawa
author_role author
author2 SOBRAL,Ana Paula Veras
SAMPAIO,Gerhilde Callou
PINTO,Isabel Maria de Araújo
SHINOHARA,Neide Kazue Sakugawa
author2_role author
author
author
author
dc.contributor.author.fl_str_mv MELO JÚNIOR,Paulo Maurício Reis de
SOBRAL,Ana Paula Veras
SAMPAIO,Gerhilde Callou
PINTO,Isabel Maria de Araújo
SHINOHARA,Neide Kazue Sakugawa
dc.subject.por.fl_str_mv Bactéria
Cimentos dentários
Products with antimicrobial action
topic Bactéria
Cimentos dentários
Products with antimicrobial action
description OBJECTIVE: The objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. METHODS: Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. RESULTS: The diameters obtained to MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) were expressed in millimeters and subsequently submitted to Tukey test (p<0.05). CONCLUSION: According to the results obtained, it was concluded that the MTA and Portland cement showed to have similar antimicrobial properties, however these were not effective against the Enterococcus faecalis.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372015000200181
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1981-863720150002000072733
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade São Leopoldo Mandic
publisher.none.fl_str_mv Faculdade São Leopoldo Mandic
dc.source.none.fl_str_mv RGO - Revista Gaúcha de Odontologia v.63 n.2 2015
reponame:RGO - Revista Gaúcha de Odontologia (Online)
instname:Faculdade São Leopoldo Mandic (FSLM)
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instname_str Faculdade São Leopoldo Mandic (FSLM)
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institution FSLM
reponame_str RGO - Revista Gaúcha de Odontologia (Online)
collection RGO - Revista Gaúcha de Odontologia (Online)
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