Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B

• Autor(a) principal: MONTEIRO,Larissa Cavalcanti
• Data de Publicação: 2019
• Outros Autores: RIBEIRO,Isabella Lima Arrais, BATISTA,Roberta Ferreti Bonan Dantas, MACIEL,Panmella Pereira, VALENÇA,Ana Maria Gondim, DULGHEROFF,Ana Carolina Bernardes, SOUZA,Joelma Rodrigues, CAVALCANTI,Yuri Wanderley, CASTELLANO,Lucio Roberto Cançado, BONAN,Paulo Rogério Ferreti
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: RGO - Revista Gaúcha de Odontologia (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372019000100309
• topic: Candida; Precursor cell lymphoblastic leukemia-lymphoma; Prevalence

ABSTRACT Objective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descriptive and analytic approach. Saliva was collected from40 individuals diagnosed with ALL and from40 healthy subjects, as a comparative group, matched by age and gender with ALL group. The mean age for both groups were 8 years-old. The isolation and identification of the Candidaspecies were performed using the CHROMagarCandidaTM and confirmed by polymerase chain reaction. The samples were subjected to antifungal susceptibility by microdilution assay for nystatin and amphotericin B. Salivary alterations and chemotherapy-induced oralmucositis were evaluated using modifiedOral Assessment Guide. Results: The positivity to Candida was higher inALL individuals (32.5%,13/40)than in a comparative group(2.5%, 1/40) (p<0.001). Candida albicans was the most prevalent strain (86.6%). The mucositis was directly associated with positive Candidacolonization (p=0.017) in the ALL group but not related with salivary alterations (p= 0.479). Six strains of C. albicans (54.5%), on ALL group, were resistant to nystatin and all strains were not susceptible to amphotericin B. Conclusion: Candida colonization was associated with ALL condition and with oral mucositis in these individuals. C. albicans was the prevalent strain and most samples were resistant to antifungal agents tested, nystatin and amphotericin B.