Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures

• Autor(a) principal: Castro-Raucci,Larissa Moreira Spinola de
• Data de Publicação: 2011
• Outros Autores: Oliveira,Ivone Regina de, Teixeira,Lucas Novaes, Rosa,Adalberto Luiz, Oliveira,Paulo Tambasco de, Jacobovitz,Marcos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Dental Journal
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000200002
• topic: calcium aluminate cement; mineral trioxide aggregate; cell culture; osteoblast; alkaline phosphatase

The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy.