Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000200002 |
Resumo: | The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy. |
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Brazilian Dental Journal |
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Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell culturescalcium aluminate cementmineral trioxide aggregatecell cultureosteoblastalkaline phosphataseThe present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy.Fundação Odontológica de Ribeirão Preto2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000200002Brazilian Dental Journal v.22 n.2 2011reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402011000200002info:eu-repo/semantics/openAccessCastro-Raucci,Larissa Moreira Spinola deOliveira,Ivone Regina deTeixeira,Lucas NovaesRosa,Adalberto LuizOliveira,Paulo Tambasco deJacobovitz,Marcoseng2011-06-08T00:00:00Zoai:scielo:S0103-64402011000200002Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2011-06-08T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
title |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
spellingShingle |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures Castro-Raucci,Larissa Moreira Spinola de calcium aluminate cement mineral trioxide aggregate cell culture osteoblast alkaline phosphatase |
title_short |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
title_full |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
title_fullStr |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
title_full_unstemmed |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
title_sort |
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures |
author |
Castro-Raucci,Larissa Moreira Spinola de |
author_facet |
Castro-Raucci,Larissa Moreira Spinola de Oliveira,Ivone Regina de Teixeira,Lucas Novaes Rosa,Adalberto Luiz Oliveira,Paulo Tambasco de Jacobovitz,Marcos |
author_role |
author |
author2 |
Oliveira,Ivone Regina de Teixeira,Lucas Novaes Rosa,Adalberto Luiz Oliveira,Paulo Tambasco de Jacobovitz,Marcos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Castro-Raucci,Larissa Moreira Spinola de Oliveira,Ivone Regina de Teixeira,Lucas Novaes Rosa,Adalberto Luiz Oliveira,Paulo Tambasco de Jacobovitz,Marcos |
dc.subject.por.fl_str_mv |
calcium aluminate cement mineral trioxide aggregate cell culture osteoblast alkaline phosphatase |
topic |
calcium aluminate cement mineral trioxide aggregate cell culture osteoblast alkaline phosphatase |
description |
The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000200002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000200002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-64402011000200002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.22 n.2 2011 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204091716730880 |