Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007 |
Resumo: | This study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time. |
id |
FUNORP-1_32208f928be45fd168f81bffdb84bdff |
---|---|
oai_identifier_str |
oai:scielo:S0103-64402012000100007 |
network_acronym_str |
FUNORP-1 |
network_name_str |
Brazilian Dental Journal |
repository_id_str |
|
spelling |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cellscell cytotoxicitycollagen membranesnitric oxideThis study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time.Fundação Odontológica de Ribeirão Preto2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007Brazilian Dental Journal v.23 n.1 2012reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402012000100007info:eu-repo/semantics/openAccessMoura,Camilla Christian GomesSoares,Priscilla Barbosa FerreiraCarneiro,Karine FernandesSouza,Maria Aparecida deMagalhães,Denildoeng2012-03-28T00:00:00Zoai:scielo:S0103-64402012000100007Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2012-03-28T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
title |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
spellingShingle |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells Moura,Camilla Christian Gomes cell cytotoxicity collagen membranes nitric oxide |
title_short |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
title_full |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
title_fullStr |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
title_full_unstemmed |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
title_sort |
Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells |
author |
Moura,Camilla Christian Gomes |
author_facet |
Moura,Camilla Christian Gomes Soares,Priscilla Barbosa Ferreira Carneiro,Karine Fernandes Souza,Maria Aparecida de Magalhães,Denildo |
author_role |
author |
author2 |
Soares,Priscilla Barbosa Ferreira Carneiro,Karine Fernandes Souza,Maria Aparecida de Magalhães,Denildo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Moura,Camilla Christian Gomes Soares,Priscilla Barbosa Ferreira Carneiro,Karine Fernandes Souza,Maria Aparecida de Magalhães,Denildo |
dc.subject.por.fl_str_mv |
cell cytotoxicity collagen membranes nitric oxide |
topic |
cell cytotoxicity collagen membranes nitric oxide |
description |
This study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-64402012000100007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.23 n.1 2012 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204092125675520 |