Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells

Detalhes bibliográficos
Autor(a) principal: Moura,Camilla Christian Gomes
Data de Publicação: 2012
Outros Autores: Soares,Priscilla Barbosa Ferreira, Carneiro,Karine Fernandes, Souza,Maria Aparecida de, Magalhães,Denildo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007
Resumo: This study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time.
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spelling Cytotoxicity of bovine and porcine collagen membranes in mononuclear cellscell cytotoxicitycollagen membranesnitric oxideThis study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time.Fundação Odontológica de Ribeirão Preto2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007Brazilian Dental Journal v.23 n.1 2012reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402012000100007info:eu-repo/semantics/openAccessMoura,Camilla Christian GomesSoares,Priscilla Barbosa FerreiraCarneiro,Karine FernandesSouza,Maria Aparecida deMagalhães,Denildoeng2012-03-28T00:00:00Zoai:scielo:S0103-64402012000100007Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2012-03-28T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
title Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
spellingShingle Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
Moura,Camilla Christian Gomes
cell cytotoxicity
collagen membranes
nitric oxide
title_short Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
title_full Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
title_fullStr Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
title_full_unstemmed Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
title_sort Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells
author Moura,Camilla Christian Gomes
author_facet Moura,Camilla Christian Gomes
Soares,Priscilla Barbosa Ferreira
Carneiro,Karine Fernandes
Souza,Maria Aparecida de
Magalhães,Denildo
author_role author
author2 Soares,Priscilla Barbosa Ferreira
Carneiro,Karine Fernandes
Souza,Maria Aparecida de
Magalhães,Denildo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Moura,Camilla Christian Gomes
Soares,Priscilla Barbosa Ferreira
Carneiro,Karine Fernandes
Souza,Maria Aparecida de
Magalhães,Denildo
dc.subject.por.fl_str_mv cell cytotoxicity
collagen membranes
nitric oxide
topic cell cytotoxicity
collagen membranes
nitric oxide
description This study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000100007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402012000100007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.23 n.1 2012
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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