Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering

Detalhes bibliográficos
Autor(a) principal: Fernandes,Ligia L.
Data de Publicação: 2011
Outros Autores: Resende,Cristiane X., Tavares,Débora S., Soares,Gloria A., Castro,Letícia O., Granjeiro,Jose M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Polímeros (São Carlos. Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282011000100003
Resumo: In this work, chitosan and collagen-chitosan porous scaffolds were produced by the freeze drying method and characterized as potential skin substitutes. Their beneficial effects on soft tissues justify the choice of both collagen and chitosan. Samples were characterized using scanning electron microscope, Fourier Transform InfraRed Spectroscopy (FTIR) and thermogravimetry (TG). The in vitro cytocompatibility of chitosan and collagen-chitosan scaffolds was evaluated with three different assays. Phenol and titanium powder were used as positive and negative controls, respectively. Scanning electron microscopy revealed the highly interconnected porous structure of the scaffolds. The addition of collagen to chitosan increased both pore diameter and porosity of the scaffolds. Results of FTIR and TG analysis indicate that the two polymers interact yielding a miscible blend with intermediate thermal degradation properties. The reduction of XTT ((2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) and the uptake of Neutral Red (NR) were not affected by the blend or by the chitosan scaffold extracts, but the blend and the titanium powder presented greater incorporation of Crystal Violet (CV) than phenol and chitosan alone. In conclusion, collagen-chitosan scaffolds produced by freeze-drying methods were cytocompatible and presented mixed properties of each component with intermediate thermal degradation properties.
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spelling Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineeringCollagenchitosanblendcytotoxicityIn this work, chitosan and collagen-chitosan porous scaffolds were produced by the freeze drying method and characterized as potential skin substitutes. Their beneficial effects on soft tissues justify the choice of both collagen and chitosan. Samples were characterized using scanning electron microscope, Fourier Transform InfraRed Spectroscopy (FTIR) and thermogravimetry (TG). The in vitro cytocompatibility of chitosan and collagen-chitosan scaffolds was evaluated with three different assays. Phenol and titanium powder were used as positive and negative controls, respectively. Scanning electron microscopy revealed the highly interconnected porous structure of the scaffolds. The addition of collagen to chitosan increased both pore diameter and porosity of the scaffolds. Results of FTIR and TG analysis indicate that the two polymers interact yielding a miscible blend with intermediate thermal degradation properties. The reduction of XTT ((2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) and the uptake of Neutral Red (NR) were not affected by the blend or by the chitosan scaffold extracts, but the blend and the titanium powder presented greater incorporation of Crystal Violet (CV) than phenol and chitosan alone. In conclusion, collagen-chitosan scaffolds produced by freeze-drying methods were cytocompatible and presented mixed properties of each component with intermediate thermal degradation properties.Associação Brasileira de Polímeros2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282011000100003Polímeros v.21 n.1 2011reponame:Polímeros (São Carlos. Online)instname:Associação Brasileira de Polímeros (ABPol)instacron:ABPO10.1590/S0104-14282011005000008info:eu-repo/semantics/openAccessFernandes,Ligia L.Resende,Cristiane X.Tavares,Débora S.Soares,Gloria A.Castro,Letícia O.Granjeiro,Jose M.eng2011-05-04T00:00:00Zoai:scielo:S0104-14282011000100003Revistahttp://www.scielo.br/pohttps://old.scielo.br/oai/scielo-oai.php||revista@abpol.org.br1678-51690104-1428opendoar:2011-05-04T00:00Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol)false
dc.title.none.fl_str_mv Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
title Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
spellingShingle Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
Fernandes,Ligia L.
Collagen
chitosan
blend
cytotoxicity
title_short Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
title_full Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
title_fullStr Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
title_full_unstemmed Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
title_sort Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering
author Fernandes,Ligia L.
author_facet Fernandes,Ligia L.
Resende,Cristiane X.
Tavares,Débora S.
Soares,Gloria A.
Castro,Letícia O.
Granjeiro,Jose M.
author_role author
author2 Resende,Cristiane X.
Tavares,Débora S.
Soares,Gloria A.
Castro,Letícia O.
Granjeiro,Jose M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes,Ligia L.
Resende,Cristiane X.
Tavares,Débora S.
Soares,Gloria A.
Castro,Letícia O.
Granjeiro,Jose M.
dc.subject.por.fl_str_mv Collagen
chitosan
blend
cytotoxicity
topic Collagen
chitosan
blend
cytotoxicity
description In this work, chitosan and collagen-chitosan porous scaffolds were produced by the freeze drying method and characterized as potential skin substitutes. Their beneficial effects on soft tissues justify the choice of both collagen and chitosan. Samples were characterized using scanning electron microscope, Fourier Transform InfraRed Spectroscopy (FTIR) and thermogravimetry (TG). The in vitro cytocompatibility of chitosan and collagen-chitosan scaffolds was evaluated with three different assays. Phenol and titanium powder were used as positive and negative controls, respectively. Scanning electron microscopy revealed the highly interconnected porous structure of the scaffolds. The addition of collagen to chitosan increased both pore diameter and porosity of the scaffolds. Results of FTIR and TG analysis indicate that the two polymers interact yielding a miscible blend with intermediate thermal degradation properties. The reduction of XTT ((2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) and the uptake of Neutral Red (NR) were not affected by the blend or by the chitosan scaffold extracts, but the blend and the titanium powder presented greater incorporation of Crystal Violet (CV) than phenol and chitosan alone. In conclusion, collagen-chitosan scaffolds produced by freeze-drying methods were cytocompatible and presented mixed properties of each component with intermediate thermal degradation properties.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282011000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282011000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-14282011005000008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Polímeros
publisher.none.fl_str_mv Associação Brasileira de Polímeros
dc.source.none.fl_str_mv Polímeros v.21 n.1 2011
reponame:Polímeros (São Carlos. Online)
instname:Associação Brasileira de Polímeros (ABPol)
instacron:ABPO
instname_str Associação Brasileira de Polímeros (ABPol)
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reponame_str Polímeros (São Carlos. Online)
collection Polímeros (São Carlos. Online)
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