Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200099 |
Resumo: | Abstract Considering that smoking is a public health problem that has been growing among adolescents, the aim of this study was to investigate the impact of cigarette smoke on osteogenic and osteoclastogenic signaling in middle palatal suture of rats. Male Wistar rats exposed (n = 30) or not to cigarette smoke (n = 30) were used. Exposure to smoke was carried out for two daily periods of 3 minutes each, with an interval of 12 hours between exposures. After the experimental periods of 3, 7, 14 and 21 days, the animals were euthanized. The collected tissues were analyzed using light microscopy and real-time RT-PCR was performed to investigate gene expression. The data obtained were compared using the Kruskal Wallis and Dunn tests (⍺ = 5%). Morphologically, there were no significant changes in the middle palatal suture of rats exposed or not to cigarette smoke during 3, 7, 14 and 21 days (p> 0.05). On the other hand, osteoclastogenic signaling was increased in animals exposed to smoke and was characterized by a higher production of RANKL at 3 and 14 days (p <0.05), with no change in the synthesis of RANK and osteoprotegerin (p> 0.05). Interestingly, in the exposed animals, an early increase in the synthesis of osteocalcin, bone sialoprotein and osteopontin was also identified at 3 days of exposure (p <0.05), not sustained over time (p> 0.05). Cigarette smoke modulates osteogenic and osteoclastogenic signaling in the middle palatal suture of young rats, although morphological changes have not been evidenced. |
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Brazilian Dental Journal |
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Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suturecigarette smokemiddle palatal suturechildrenAbstract Considering that smoking is a public health problem that has been growing among adolescents, the aim of this study was to investigate the impact of cigarette smoke on osteogenic and osteoclastogenic signaling in middle palatal suture of rats. Male Wistar rats exposed (n = 30) or not to cigarette smoke (n = 30) were used. Exposure to smoke was carried out for two daily periods of 3 minutes each, with an interval of 12 hours between exposures. After the experimental periods of 3, 7, 14 and 21 days, the animals were euthanized. The collected tissues were analyzed using light microscopy and real-time RT-PCR was performed to investigate gene expression. The data obtained were compared using the Kruskal Wallis and Dunn tests (⍺ = 5%). Morphologically, there were no significant changes in the middle palatal suture of rats exposed or not to cigarette smoke during 3, 7, 14 and 21 days (p> 0.05). On the other hand, osteoclastogenic signaling was increased in animals exposed to smoke and was characterized by a higher production of RANKL at 3 and 14 days (p <0.05), with no change in the synthesis of RANK and osteoprotegerin (p> 0.05). Interestingly, in the exposed animals, an early increase in the synthesis of osteocalcin, bone sialoprotein and osteopontin was also identified at 3 days of exposure (p <0.05), not sustained over time (p> 0.05). Cigarette smoke modulates osteogenic and osteoclastogenic signaling in the middle palatal suture of young rats, although morphological changes have not been evidenced.Fundação Odontológica de Ribeirão Preto2022-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200099Brazilian Dental Journal v.33 n.2 2022reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202203966info:eu-repo/semantics/openAccessArnez,Maya Fernanda ManfrinMonteiro,Patrícia MariaPaula-Silva,Francisco Wanderley GarciaDessotti,Gabriel BarrettoMenezes,Luciane Macedo deKüchler,Erika CalvanoAlves,Sandra Yasuyo FukadaMatsumoto,Mirian Aiko NakaneStuani,Maria Bernadete Sassoeng2022-04-26T00:00:00Zoai:scielo:S0103-64402022000200099Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2022-04-26T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
title |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
spellingShingle |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture Arnez,Maya Fernanda Manfrin cigarette smoke middle palatal suture children |
title_short |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
title_full |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
title_fullStr |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
title_full_unstemmed |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
title_sort |
Impact of cigarette smoke on osteogenic and osteoclast signaling in middle palatal suture |
author |
Arnez,Maya Fernanda Manfrin |
author_facet |
Arnez,Maya Fernanda Manfrin Monteiro,Patrícia Maria Paula-Silva,Francisco Wanderley Garcia Dessotti,Gabriel Barretto Menezes,Luciane Macedo de Küchler,Erika Calvano Alves,Sandra Yasuyo Fukada Matsumoto,Mirian Aiko Nakane Stuani,Maria Bernadete Sasso |
author_role |
author |
author2 |
Monteiro,Patrícia Maria Paula-Silva,Francisco Wanderley Garcia Dessotti,Gabriel Barretto Menezes,Luciane Macedo de Küchler,Erika Calvano Alves,Sandra Yasuyo Fukada Matsumoto,Mirian Aiko Nakane Stuani,Maria Bernadete Sasso |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Arnez,Maya Fernanda Manfrin Monteiro,Patrícia Maria Paula-Silva,Francisco Wanderley Garcia Dessotti,Gabriel Barretto Menezes,Luciane Macedo de Küchler,Erika Calvano Alves,Sandra Yasuyo Fukada Matsumoto,Mirian Aiko Nakane Stuani,Maria Bernadete Sasso |
dc.subject.por.fl_str_mv |
cigarette smoke middle palatal suture children |
topic |
cigarette smoke middle palatal suture children |
description |
Abstract Considering that smoking is a public health problem that has been growing among adolescents, the aim of this study was to investigate the impact of cigarette smoke on osteogenic and osteoclastogenic signaling in middle palatal suture of rats. Male Wistar rats exposed (n = 30) or not to cigarette smoke (n = 30) were used. Exposure to smoke was carried out for two daily periods of 3 minutes each, with an interval of 12 hours between exposures. After the experimental periods of 3, 7, 14 and 21 days, the animals were euthanized. The collected tissues were analyzed using light microscopy and real-time RT-PCR was performed to investigate gene expression. The data obtained were compared using the Kruskal Wallis and Dunn tests (⍺ = 5%). Morphologically, there were no significant changes in the middle palatal suture of rats exposed or not to cigarette smoke during 3, 7, 14 and 21 days (p> 0.05). On the other hand, osteoclastogenic signaling was increased in animals exposed to smoke and was characterized by a higher production of RANKL at 3 and 14 days (p <0.05), with no change in the synthesis of RANK and osteoprotegerin (p> 0.05). Interestingly, in the exposed animals, an early increase in the synthesis of osteocalcin, bone sialoprotein and osteopontin was also identified at 3 days of exposure (p <0.05), not sustained over time (p> 0.05). Cigarette smoke modulates osteogenic and osteoclastogenic signaling in the middle palatal suture of young rats, although morphological changes have not been evidenced. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200099 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440202203966 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.33 n.2 2022 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204096632455168 |