An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004 |
Resumo: | The aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts. |
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Brazilian Dental Journal |
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An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblastsendodontic sealersfibrosisbiocompatibilitymyofibroblastsThe aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts.Fundação Odontológica de Ribeirão Preto2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004Brazilian Dental Journal v.22 n.5 2011reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402011000500004info:eu-repo/semantics/openAccessGiovanini,Allan FernandoLeonardi,Denise PiottoBaratto-Filho,FlaresValença,Paola CristineMoresca,Ricardo CésarMoro,AlexandreSchramm,Celso Alfredoeng2011-10-14T00:00:00Zoai:scielo:S0103-64402011000500004Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2011-10-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
title |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
spellingShingle |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts Giovanini,Allan Fernando endodontic sealers fibrosis biocompatibility myofibroblasts |
title_short |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
title_full |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
title_fullStr |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
title_full_unstemmed |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
title_sort |
An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts |
author |
Giovanini,Allan Fernando |
author_facet |
Giovanini,Allan Fernando Leonardi,Denise Piotto Baratto-Filho,Flares Valença,Paola Cristine Moresca,Ricardo César Moro,Alexandre Schramm,Celso Alfredo |
author_role |
author |
author2 |
Leonardi,Denise Piotto Baratto-Filho,Flares Valença,Paola Cristine Moresca,Ricardo César Moro,Alexandre Schramm,Celso Alfredo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Giovanini,Allan Fernando Leonardi,Denise Piotto Baratto-Filho,Flares Valença,Paola Cristine Moresca,Ricardo César Moro,Alexandre Schramm,Celso Alfredo |
dc.subject.por.fl_str_mv |
endodontic sealers fibrosis biocompatibility myofibroblasts |
topic |
endodontic sealers fibrosis biocompatibility myofibroblasts |
description |
The aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-64402011000500004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.22 n.5 2011 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204091819491328 |