An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts

Detalhes bibliográficos
Autor(a) principal: Giovanini,Allan Fernando
Data de Publicação: 2011
Outros Autores: Leonardi,Denise Piotto, Baratto-Filho,Flares, Valença,Paola Cristine, Moresca,Ricardo César, Moro,Alexandre, Schramm,Celso Alfredo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004
Resumo: The aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts.
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spelling An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblastsendodontic sealersfibrosisbiocompatibilitymyofibroblastsThe aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts.Fundação Odontológica de Ribeirão Preto2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004Brazilian Dental Journal v.22 n.5 2011reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402011000500004info:eu-repo/semantics/openAccessGiovanini,Allan FernandoLeonardi,Denise PiottoBaratto-Filho,FlaresValença,Paola CristineMoresca,Ricardo CésarMoro,AlexandreSchramm,Celso Alfredoeng2011-10-14T00:00:00Zoai:scielo:S0103-64402011000500004Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2011-10-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
title An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
spellingShingle An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
Giovanini,Allan Fernando
endodontic sealers
fibrosis
biocompatibility
myofibroblasts
title_short An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
title_full An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
title_fullStr An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
title_full_unstemmed An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
title_sort An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts
author Giovanini,Allan Fernando
author_facet Giovanini,Allan Fernando
Leonardi,Denise Piotto
Baratto-Filho,Flares
Valença,Paola Cristine
Moresca,Ricardo César
Moro,Alexandre
Schramm,Celso Alfredo
author_role author
author2 Leonardi,Denise Piotto
Baratto-Filho,Flares
Valença,Paola Cristine
Moresca,Ricardo César
Moro,Alexandre
Schramm,Celso Alfredo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Giovanini,Allan Fernando
Leonardi,Denise Piotto
Baratto-Filho,Flares
Valença,Paola Cristine
Moresca,Ricardo César
Moro,Alexandre
Schramm,Celso Alfredo
dc.subject.por.fl_str_mv endodontic sealers
fibrosis
biocompatibility
myofibroblasts
topic endodontic sealers
fibrosis
biocompatibility
myofibroblasts
description The aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402011000500004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.22 n.5 2011
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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