Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study

Detalhes bibliográficos
Autor(a) principal: Pinheiro,Antonio Luiz Barbosa
Data de Publicação: 2002
Outros Autores: Nascimento,Silene Carneiro do, Vieira,Alessandro Leonardo de Barros, Rolim,Aluízio Barros, Silva,Pedro Soriano da, Brugnera Jr.,Aldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200006
Resumo: Low level laser therapy (LLLT) has been used successfully in biomedicine and some of the results are thought to be related to cell proliferation. The effects of LLLT on cell proliferation is debatable because studies have found both an increase and a decrease in proliferation of cell cultures. Cell culture is an excellent method to assess both effects and dose of treatment. The aim of this study was to assess the effect of 635nm and 670nm laser irradiation of H.Ep.2 cells in vitro using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). The cells were obtained from squamous cell carcinoma (SCC) of the larynx and were routinely processed from defrost to the experimental condition. Twenty-four hours after transplantation the cells were irradiated with doses ranging from 0.04 to 0.48J/cm² for seven consecutive days (5 mW diode lasers: 635nm or 670nm, beam cross-section ~1mm) at local light doses between 0.04 and 0.48J/cm². The results showed that 635nm laser light did not significantly stimulate the proliferation of H.Ep.2 cells at doses of 0.04J/cm² to 0.48J/cm², However, 670nm laser irradiation led to an increased cell proliferation when compared to both control and 635nm irradiated cells. The best cell proliferation was found with 670nm laser irradiated cultures exposed to doses of doses of 0.04 to 0.48J/cm². We conclude that both dose and wavelength are factors that may affect cell proliferation of H.Ep.2 cells.
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spelling Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" studycell proliferationhazardsstimulatory effectLow level laser therapy (LLLT) has been used successfully in biomedicine and some of the results are thought to be related to cell proliferation. The effects of LLLT on cell proliferation is debatable because studies have found both an increase and a decrease in proliferation of cell cultures. Cell culture is an excellent method to assess both effects and dose of treatment. The aim of this study was to assess the effect of 635nm and 670nm laser irradiation of H.Ep.2 cells in vitro using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). The cells were obtained from squamous cell carcinoma (SCC) of the larynx and were routinely processed from defrost to the experimental condition. Twenty-four hours after transplantation the cells were irradiated with doses ranging from 0.04 to 0.48J/cm² for seven consecutive days (5 mW diode lasers: 635nm or 670nm, beam cross-section ~1mm) at local light doses between 0.04 and 0.48J/cm². The results showed that 635nm laser light did not significantly stimulate the proliferation of H.Ep.2 cells at doses of 0.04J/cm² to 0.48J/cm², However, 670nm laser irradiation led to an increased cell proliferation when compared to both control and 635nm irradiated cells. The best cell proliferation was found with 670nm laser irradiated cultures exposed to doses of doses of 0.04 to 0.48J/cm². We conclude that both dose and wavelength are factors that may affect cell proliferation of H.Ep.2 cells.Fundação Odontológica de Ribeirão Preto2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200006Brazilian Dental Journal v.13 n.2 2002reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402002000200006info:eu-repo/semantics/openAccessPinheiro,Antonio Luiz BarbosaNascimento,Silene Carneiro doVieira,Alessandro Leonardo de BarrosRolim,Aluízio BarrosSilva,Pedro Soriano daBrugnera Jr.,Aldoeng2003-04-04T00:00:00Zoai:scielo:S0103-64402002000200006Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2003-04-04T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
title Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
spellingShingle Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
Pinheiro,Antonio Luiz Barbosa
cell proliferation
hazards
stimulatory effect
title_short Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
title_full Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
title_fullStr Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
title_full_unstemmed Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
title_sort Does LLLT stimulate laryngeal carcinoma cells? An "in vitro" study
author Pinheiro,Antonio Luiz Barbosa
author_facet Pinheiro,Antonio Luiz Barbosa
Nascimento,Silene Carneiro do
Vieira,Alessandro Leonardo de Barros
Rolim,Aluízio Barros
Silva,Pedro Soriano da
Brugnera Jr.,Aldo
author_role author
author2 Nascimento,Silene Carneiro do
Vieira,Alessandro Leonardo de Barros
Rolim,Aluízio Barros
Silva,Pedro Soriano da
Brugnera Jr.,Aldo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro,Antonio Luiz Barbosa
Nascimento,Silene Carneiro do
Vieira,Alessandro Leonardo de Barros
Rolim,Aluízio Barros
Silva,Pedro Soriano da
Brugnera Jr.,Aldo
dc.subject.por.fl_str_mv cell proliferation
hazards
stimulatory effect
topic cell proliferation
hazards
stimulatory effect
description Low level laser therapy (LLLT) has been used successfully in biomedicine and some of the results are thought to be related to cell proliferation. The effects of LLLT on cell proliferation is debatable because studies have found both an increase and a decrease in proliferation of cell cultures. Cell culture is an excellent method to assess both effects and dose of treatment. The aim of this study was to assess the effect of 635nm and 670nm laser irradiation of H.Ep.2 cells in vitro using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). The cells were obtained from squamous cell carcinoma (SCC) of the larynx and were routinely processed from defrost to the experimental condition. Twenty-four hours after transplantation the cells were irradiated with doses ranging from 0.04 to 0.48J/cm² for seven consecutive days (5 mW diode lasers: 635nm or 670nm, beam cross-section ~1mm) at local light doses between 0.04 and 0.48J/cm². The results showed that 635nm laser light did not significantly stimulate the proliferation of H.Ep.2 cells at doses of 0.04J/cm² to 0.48J/cm², However, 670nm laser irradiation led to an increased cell proliferation when compared to both control and 635nm irradiated cells. The best cell proliferation was found with 670nm laser irradiated cultures exposed to doses of doses of 0.04 to 0.48J/cm². We conclude that both dose and wavelength are factors that may affect cell proliferation of H.Ep.2 cells.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402002000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402002000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.13 n.2 2002
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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