Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study

Detalhes bibliográficos
Autor(a) principal: Pinheiro, Antonio Luiz Barbosa
Data de Publicação: 2002
Outros Autores: Nascimento, Silene Carneiro do, Vieira, Alessandro Leonardo de Barros, Rolim, Aluízio Barros, Silva, Pedro Soriano da, Brugnera Júnior, Aldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5046
Resumo: p.109-112
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spelling Pinheiro, Antonio Luiz BarbosaNascimento, Silene Carneiro doVieira, Alessandro Leonardo de BarrosRolim, Aluízio BarrosSilva, Pedro Soriano daBrugnera Júnior, AldoPinheiro, Antonio Luiz BarbosaNascimento, Silene Carneiro doVieira, Alessandro Leonardo de BarrosRolim, Aluízio BarrosSilva, Pedro Soriano daBrugnera Júnior, Aldo2012-01-06T12:29:20Z2012-01-06T12:29:20Z20020103-6440http://www.repositorio.ufba.br/ri/handle/ri/504613(2)p.109-112Low level laser therapy (LLLT) has been used successfully in biomedicine and some of the results are thought to be related to cell proliferation. The effects of LLLT on cell proliferation is debatable because studies have found both an increase and a decrease in proliferation of cell cultures. Cell culture is an excellent method to assess both effects and dose of treatment. The aim of this study was to assess the effect of 635nm and 670nm laser irradiation of H.Ep.2 cells in vitro using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide). The cells were obtained from squamous cell carcinoma (SCC) of the larynx and were routinely processed from defrost to the experimental condition. Twenty-four hours after transplantation the cells were irradiated with doses ranging from 0.04 to 0.48J/cm2 for seven consecutive days (5 mW diode lasers: 635nm or 670nm, beam cross-section ~1mm) at local light doses between 0.04 and 0.48J/cm2. The results showed that 635nm laser light did not significantly stimulate the proliferation of H.Ep.2 cells at doses of 0.04J/cm2 to 0.48J/cm2, However, 670nm laser irradiation led to an increased cell proliferation when compared to both control and 635nm irradiated cells. The best cell proliferation was found with 670nm laser irradiated cultures exposed to doses of doses of 0.04 to 0.48J/cm2. We conclude that both dose and wavelength are factors that may affect cell proliferation of H.Ep.2 cells.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-06T12:29:20Z No. of bitstreams: 1 v13n2a06.pdf: 29662 bytes, checksum: 921ef6f246c0d71aeedc74ffb7ccddfb (MD5)Made available in DSpace on 2012-01-06T12:29:20Z (GMT). No. of bitstreams: 1 v13n2a06.pdf: 29662 bytes, checksum: 921ef6f246c0d71aeedc74ffb7ccddfb (MD5) Previous issue date: 2002cell proliferationhazardsstimulatory effectDoes LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro StudyBrazilian Dental Journalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALv13n2a06.pdfv13n2a06.pdfapplication/pdf29662https://repositorio.ufba.br/bitstream/ri/5046/1/v13n2a06.pdf921ef6f246c0d71aeedc74ffb7ccddfbMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5046/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTv13n2a06.pdf.txtv13n2a06.pdf.txtExtracted texttext/plain16604https://repositorio.ufba.br/bitstream/ri/5046/3/v13n2a06.pdf.txtb43e2e9b2eb03113c5f4e214542de2c3MD53ri/50462022-07-05 14:03:44.101oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:44Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
dc.title.alternative.pt_BR.fl_str_mv Brazilian Dental Journal
title Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
spellingShingle Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
Pinheiro, Antonio Luiz Barbosa
cell proliferation
hazards
stimulatory effect
title_short Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
title_full Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
title_fullStr Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
title_full_unstemmed Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
title_sort Does LLLT Stimulate Laryngeal Carcinoma Cells? An In Vitro Study
author Pinheiro, Antonio Luiz Barbosa
author_facet Pinheiro, Antonio Luiz Barbosa
Nascimento, Silene Carneiro do
Vieira, Alessandro Leonardo de Barros
Rolim, Aluízio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
author_role author
author2 Nascimento, Silene Carneiro do
Vieira, Alessandro Leonardo de Barros
Rolim, Aluízio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro, Antonio Luiz Barbosa
Nascimento, Silene Carneiro do
Vieira, Alessandro Leonardo de Barros
Rolim, Aluízio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
Pinheiro, Antonio Luiz Barbosa
Nascimento, Silene Carneiro do
Vieira, Alessandro Leonardo de Barros
Rolim, Aluízio Barros
Silva, Pedro Soriano da
Brugnera Júnior, Aldo
dc.subject.por.fl_str_mv cell proliferation
hazards
stimulatory effect
topic cell proliferation
hazards
stimulatory effect
description p.109-112
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2012-01-06T12:29:20Z
dc.date.available.fl_str_mv 2012-01-06T12:29:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5046
dc.identifier.issn.none.fl_str_mv 0103-6440
dc.identifier.number.pt_BR.fl_str_mv 13(2)
identifier_str_mv 0103-6440
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url http://www.repositorio.ufba.br/ri/handle/ri/5046
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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