Hypomethylation of tumor suppressor genes in odontogenic myxoma

Detalhes bibliográficos
Autor(a) principal: Moreira,Paula Rocha
Data de Publicação: 2011
Outros Autores: Cardoso,Fabiano Pereira, Brito,João Artur Ricieri, Batista,Aline Carvalho, Gomes,Carolina Cavaliéri, Gomez,Ricardo Santiago
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500013
Resumo: Odontogenic myxoma (OM) is an ectomesenchymal benign odontogenic tumor characterized by spindle or stellate-shaped cells embedded in an abundant myxoid or mucoid extracellular matrix. DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands in the promoter gene. DNA methylation can decrease the expression of tumor suppressor genes and contribute to the development of neoplastic lesions. The aim of study was to evaluate the methylation pattern of the tumor suppressor genes P16 (CDKN2A), P21 (CDKN1A), P27 (CDKN1B), P53 (TP53) and RB1 in OM and dental pulp. Methylation was evaluated using methylation-specific polymerase chain reaction (PCR). The transcription was studied in some cases by using reverse transcription quantitative PCR. A higher frequency of unmethylated P27, P53, and RB1 samples was observed in the OM when compared with the dental pulp. OM expressed mRNA of all the genes evaluated. Considering all the samples together, the expression of Rb was higher in the unmethylated samples compared with the partially methylated samples. This investigation revealed hypomethylation of the genes P27, P53, and RB1 in OM. In addition, methylation of tumor suppressor genes was found to be an usual event in normal dental pulp.
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spelling Hypomethylation of tumor suppressor genes in odontogenic myxomamethylationtumor suppressor genesodontogenic myxomadental pulpOdontogenic myxoma (OM) is an ectomesenchymal benign odontogenic tumor characterized by spindle or stellate-shaped cells embedded in an abundant myxoid or mucoid extracellular matrix. DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands in the promoter gene. DNA methylation can decrease the expression of tumor suppressor genes and contribute to the development of neoplastic lesions. The aim of study was to evaluate the methylation pattern of the tumor suppressor genes P16 (CDKN2A), P21 (CDKN1A), P27 (CDKN1B), P53 (TP53) and RB1 in OM and dental pulp. Methylation was evaluated using methylation-specific polymerase chain reaction (PCR). The transcription was studied in some cases by using reverse transcription quantitative PCR. A higher frequency of unmethylated P27, P53, and RB1 samples was observed in the OM when compared with the dental pulp. OM expressed mRNA of all the genes evaluated. Considering all the samples together, the expression of Rb was higher in the unmethylated samples compared with the partially methylated samples. This investigation revealed hypomethylation of the genes P27, P53, and RB1 in OM. In addition, methylation of tumor suppressor genes was found to be an usual event in normal dental pulp.Fundação Odontológica de Ribeirão Preto2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500013Brazilian Dental Journal v.22 n.5 2011reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402011000500013info:eu-repo/semantics/openAccessMoreira,Paula RochaCardoso,Fabiano PereiraBrito,João Artur RicieriBatista,Aline CarvalhoGomes,Carolina CavaliériGomez,Ricardo Santiagoeng2011-10-14T00:00:00Zoai:scielo:S0103-64402011000500013Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2011-10-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Hypomethylation of tumor suppressor genes in odontogenic myxoma
title Hypomethylation of tumor suppressor genes in odontogenic myxoma
spellingShingle Hypomethylation of tumor suppressor genes in odontogenic myxoma
Moreira,Paula Rocha
methylation
tumor suppressor genes
odontogenic myxoma
dental pulp
title_short Hypomethylation of tumor suppressor genes in odontogenic myxoma
title_full Hypomethylation of tumor suppressor genes in odontogenic myxoma
title_fullStr Hypomethylation of tumor suppressor genes in odontogenic myxoma
title_full_unstemmed Hypomethylation of tumor suppressor genes in odontogenic myxoma
title_sort Hypomethylation of tumor suppressor genes in odontogenic myxoma
author Moreira,Paula Rocha
author_facet Moreira,Paula Rocha
Cardoso,Fabiano Pereira
Brito,João Artur Ricieri
Batista,Aline Carvalho
Gomes,Carolina Cavaliéri
Gomez,Ricardo Santiago
author_role author
author2 Cardoso,Fabiano Pereira
Brito,João Artur Ricieri
Batista,Aline Carvalho
Gomes,Carolina Cavaliéri
Gomez,Ricardo Santiago
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira,Paula Rocha
Cardoso,Fabiano Pereira
Brito,João Artur Ricieri
Batista,Aline Carvalho
Gomes,Carolina Cavaliéri
Gomez,Ricardo Santiago
dc.subject.por.fl_str_mv methylation
tumor suppressor genes
odontogenic myxoma
dental pulp
topic methylation
tumor suppressor genes
odontogenic myxoma
dental pulp
description Odontogenic myxoma (OM) is an ectomesenchymal benign odontogenic tumor characterized by spindle or stellate-shaped cells embedded in an abundant myxoid or mucoid extracellular matrix. DNA methylation is characterized by the addition of methyl groups in cytosines within CpG islands in the promoter gene. DNA methylation can decrease the expression of tumor suppressor genes and contribute to the development of neoplastic lesions. The aim of study was to evaluate the methylation pattern of the tumor suppressor genes P16 (CDKN2A), P21 (CDKN1A), P27 (CDKN1B), P53 (TP53) and RB1 in OM and dental pulp. Methylation was evaluated using methylation-specific polymerase chain reaction (PCR). The transcription was studied in some cases by using reverse transcription quantitative PCR. A higher frequency of unmethylated P27, P53, and RB1 samples was observed in the OM when compared with the dental pulp. OM expressed mRNA of all the genes evaluated. Considering all the samples together, the expression of Rb was higher in the unmethylated samples compared with the partially methylated samples. This investigation revealed hypomethylation of the genes P27, P53, and RB1 in OM. In addition, methylation of tumor suppressor genes was found to be an usual event in normal dental pulp.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000500013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402011000500013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.22 n.5 2011
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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