Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma

Detalhes bibliográficos
Autor(a) principal: Miyagi,Sueli Patricia Harumi
Data de Publicação: 2012
Outros Autores: Maranduba,Carlos Magno da Costa, Silva,Fernando de Sá, Marques,Márcia Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000200009
Resumo: Little is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.
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spelling Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxomaStem CellsDental PulpMyxomaOdontogenic TumorsLittle is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000200009Brazilian Oral Research v.26 n.2 2012reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242012000200009info:eu-repo/semantics/openAccessMiyagi,Sueli Patricia HarumiMaranduba,Carlos Magno da CostaSilva,Fernando de SáMarques,Márcia Martinseng2012-04-10T00:00:00Zoai:scielo:S1806-83242012000200009Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2012-04-10T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
title Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
spellingShingle Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
Miyagi,Sueli Patricia Harumi
Stem Cells
Dental Pulp
Myxoma
Odontogenic Tumors
title_short Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
title_full Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
title_fullStr Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
title_full_unstemmed Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
title_sort Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma
author Miyagi,Sueli Patricia Harumi
author_facet Miyagi,Sueli Patricia Harumi
Maranduba,Carlos Magno da Costa
Silva,Fernando de Sá
Marques,Márcia Martins
author_role author
author2 Maranduba,Carlos Magno da Costa
Silva,Fernando de Sá
Marques,Márcia Martins
author2_role author
author
author
dc.contributor.author.fl_str_mv Miyagi,Sueli Patricia Harumi
Maranduba,Carlos Magno da Costa
Silva,Fernando de Sá
Marques,Márcia Martins
dc.subject.por.fl_str_mv Stem Cells
Dental Pulp
Myxoma
Odontogenic Tumors
topic Stem Cells
Dental Pulp
Myxoma
Odontogenic Tumors
description Little is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000200009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-83242012000200009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.26 n.2 2012
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron:SBPQO
instname_str Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
instacron_str SBPQO
institution SBPQO
reponame_str Brazilian Oral Research
collection Brazilian Oral Research
repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
repository.mail.fl_str_mv pob@edu.usp.br||bor@sbpqo.org.br
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