Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study

Detalhes bibliográficos
Autor(a) principal: Cohenca,Nestor
Data de Publicação: 2013
Outros Autores: Silva,Lea Assed Bezerra, Silva,Raquel Assed Bezerra, Nelson-Filho,Paulo, Heilborn,Carlos, Watanabe,Evandro, Saraiva,Maria Conceição Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467
Resumo: The present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control – sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction.
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spelling Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Studyroot canal disinfectionirrigationpassive ultrasonic irrigationapical negative pressureThe present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control – sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction.Fundação Odontológica de Ribeirão Preto2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467Brazilian Dental Journal v.24 n.5 2013reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201302179info:eu-repo/semantics/openAccessCohenca,NestorSilva,Lea Assed BezerraSilva,Raquel Assed BezerraNelson-Filho,PauloHeilborn,CarlosWatanabe,EvandroSaraiva,Maria Conceição Pereiraeng2015-10-27T00:00:00Zoai:scielo:S0103-64402013000500467Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2015-10-27T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
title Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
spellingShingle Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
Cohenca,Nestor
root canal disinfection
irrigation
passive ultrasonic irrigation
apical negative pressure
title_short Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
title_full Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
title_fullStr Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
title_full_unstemmed Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
title_sort Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
author Cohenca,Nestor
author_facet Cohenca,Nestor
Silva,Lea Assed Bezerra
Silva,Raquel Assed Bezerra
Nelson-Filho,Paulo
Heilborn,Carlos
Watanabe,Evandro
Saraiva,Maria Conceição Pereira
author_role author
author2 Silva,Lea Assed Bezerra
Silva,Raquel Assed Bezerra
Nelson-Filho,Paulo
Heilborn,Carlos
Watanabe,Evandro
Saraiva,Maria Conceição Pereira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cohenca,Nestor
Silva,Lea Assed Bezerra
Silva,Raquel Assed Bezerra
Nelson-Filho,Paulo
Heilborn,Carlos
Watanabe,Evandro
Saraiva,Maria Conceição Pereira
dc.subject.por.fl_str_mv root canal disinfection
irrigation
passive ultrasonic irrigation
apical negative pressure
topic root canal disinfection
irrigation
passive ultrasonic irrigation
apical negative pressure
description The present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control – sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440201302179
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.24 n.5 2013
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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