Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467 |
Resumo: | The present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control – sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction. |
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Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Studyroot canal disinfectionirrigationpassive ultrasonic irrigationapical negative pressureThe present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control – sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction.Fundação Odontológica de Ribeirão Preto2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467Brazilian Dental Journal v.24 n.5 2013reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201302179info:eu-repo/semantics/openAccessCohenca,NestorSilva,Lea Assed BezerraSilva,Raquel Assed BezerraNelson-Filho,PauloHeilborn,CarlosWatanabe,EvandroSaraiva,Maria Conceição Pereiraeng2015-10-27T00:00:00Zoai:scielo:S0103-64402013000500467Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2015-10-27T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
title |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
spellingShingle |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study Cohenca,Nestor root canal disinfection irrigation passive ultrasonic irrigation apical negative pressure |
title_short |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
title_full |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
title_fullStr |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
title_full_unstemmed |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
title_sort |
Microbiological Evaluation of Different Irrigation Protocols on Root Canal Disinfection in Teeth with Apical Periodontitis: An In Vivo Study |
author |
Cohenca,Nestor |
author_facet |
Cohenca,Nestor Silva,Lea Assed Bezerra Silva,Raquel Assed Bezerra Nelson-Filho,Paulo Heilborn,Carlos Watanabe,Evandro Saraiva,Maria Conceição Pereira |
author_role |
author |
author2 |
Silva,Lea Assed Bezerra Silva,Raquel Assed Bezerra Nelson-Filho,Paulo Heilborn,Carlos Watanabe,Evandro Saraiva,Maria Conceição Pereira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cohenca,Nestor Silva,Lea Assed Bezerra Silva,Raquel Assed Bezerra Nelson-Filho,Paulo Heilborn,Carlos Watanabe,Evandro Saraiva,Maria Conceição Pereira |
dc.subject.por.fl_str_mv |
root canal disinfection irrigation passive ultrasonic irrigation apical negative pressure |
topic |
root canal disinfection irrigation passive ultrasonic irrigation apical negative pressure |
description |
The present in vivo study was designed to evaluate the efficacy of apical negative pressure irrigation (ANP), passive ultrasonic irrigation (PUI) and positive pressure irrigation (PP) in the reduction of intracanal bacteria of dogs' teeth with pulp necrosis and apical periodontitis. Eighty root canals were randomly distributed into 3 experimental and 2 control groups according to the irrigation delivery system: group ANP (n=20), group PUI (n=20), group PP (n=20), group PC (positive control – sterile saline irrigation; n=10) and group NC (negative control - vital pulps not subjected to bacterial inoculation; n=10). The first sample (S1) was collected at baseline, and the second sample (S2) was collected after the disinfection protocols. All samples were seeded in culture media for anaerobic bacteria. CFU counts were analyzed statistically by the Kruskal-Wallis, Dwass-Steel-Critchlow-Fligner post-hoc and Chi-square followed by Tukey like multiple comparisons for proportions (α=0.05). All experimental groups were effective in reducing Gram-positive bacteria compared with PC (p<0.05). Regarding the reduction of Gram-negative bacteria, group ANP was significantly better than PP (p<0.05). No statistically significant difference could be found between PP and PUI (p>0.05). In dog's teeth with apical periodontitis, the use of ANP and PUI can be considered promising disinfection protocols as both delivery systems promoted a significant bacterial reduction. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000500467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201302179 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.24 n.5 2013 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204092710780928 |