Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201300022 http://hdl.handle.net/11449/172016 |
Resumo: | Endodontic irrigation aims to clean and disinfect the root canal system. Passive ultrasonic irrigation (PUI) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. The aim of this study was to evaluate, ex vivo, the effectiveness of PUI in eliminating Enterococcus faecalis from root canals. Seventy-five extracted human single-root teeth were used. After root canal preparation, specimens were inoculated with E. faecalis and incubated at 37 °C for 21 days. Specimens were distributed into five groups (n=15), according to the irrigation method: PUI + saline solution (PUI/SS); PUI + 1% NaOCl (PUI/NaOCl); conventional needle irrigation (CNI) + saline solution (CNI/SS); CNI + 1% NaOCl (CNI/NaOCl); No irrigation (control). Microbiological samples were collected at three time points: initial (21 days after inoculation), post-irrigation (immediately after irrigation), and final (7 days after irrigation). Data were obtained in CFU mL-1 and subjected to analysis by ANOVA and Tukey’s tests at 5% significance level. The post-irrigation samples did not demonstrate statistical difference between PUI/SS and CNI/SS nor between PUI/NaOCl and CNI/NaOCl (p>0.05), but PUI/NaOCl and CNI/NaOCl had lower CFU mL-1 number than the other groups (p>0.05). Statistically significant difference was observed between the initial and post-irrigation samples and between the post-irrigation and final samples (p<0.05) in all groups, except in the control. The final samples of all groups presented bacterial counts similar to the initial samples. PUI or CNI with 1% NaOCl contribute to disinfection, but are unable to eradicate E. faecalis from the root canal system. |
id |
UNSP_f81e8f0b3097648391944b3c827f3545 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/172016 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo studyEndodonticsEnterococcus faecalisPassive ultrasonic irrigationRoot canal disinfectionEndodontic irrigation aims to clean and disinfect the root canal system. Passive ultrasonic irrigation (PUI) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. The aim of this study was to evaluate, ex vivo, the effectiveness of PUI in eliminating Enterococcus faecalis from root canals. Seventy-five extracted human single-root teeth were used. After root canal preparation, specimens were inoculated with E. faecalis and incubated at 37 °C for 21 days. Specimens were distributed into five groups (n=15), according to the irrigation method: PUI + saline solution (PUI/SS); PUI + 1% NaOCl (PUI/NaOCl); conventional needle irrigation (CNI) + saline solution (CNI/SS); CNI + 1% NaOCl (CNI/NaOCl); No irrigation (control). Microbiological samples were collected at three time points: initial (21 days after inoculation), post-irrigation (immediately after irrigation), and final (7 days after irrigation). Data were obtained in CFU mL-1 and subjected to analysis by ANOVA and Tukey’s tests at 5% significance level. The post-irrigation samples did not demonstrate statistical difference between PUI/SS and CNI/SS nor between PUI/NaOCl and CNI/NaOCl (p>0.05), but PUI/NaOCl and CNI/NaOCl had lower CFU mL-1 number than the other groups (p>0.05). Statistically significant difference was observed between the initial and post-irrigation samples and between the post-irrigation and final samples (p<0.05) in all groups, except in the control. The final samples of all groups presented bacterial counts similar to the initial samples. PUI or CNI with 1% NaOCl contribute to disinfection, but are unable to eradicate E. faecalis from the root canal system.Department of Restorative Dentistry, Araraquara Dental School, UNESP - Universidade Estadual PaulistaDepartment of Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São PauloDepartment of Restorative Dentistry, Araraquara Dental School, UNESP - Universidade Estadual PaulistaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Guerreiro-Tanomaru, Juliane Maria [UNESP]Chávez-Andrade, Gisselle Moraima [UNESP]de Faria-Júnior, Norberto Batista [UNESP]Watanabe, EvandroTanomaru-Filho, Mário [UNESP]2018-12-11T16:58:09Z2018-12-11T16:58:09Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article342-346application/pdfhttp://dx.doi.org/10.1590/0103-6440201300022Brazilian Dental Journal, v. 26, n. 4, p. 342-346, 2015.1806-47600103-6440http://hdl.handle.net/11449/17201610.1590/0103-6440201300022S0103-644020150004003422-s2.0-84940032448S0103-64402015000400342.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2024-01-04T06:24:17Zoai:repositorio.unesp.br:11449/172016Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-04T06:24:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
title |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
spellingShingle |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study Guerreiro-Tanomaru, Juliane Maria [UNESP] Endodontics Enterococcus faecalis Passive ultrasonic irrigation Root canal disinfection |
title_short |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
title_full |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
title_fullStr |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
title_full_unstemmed |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
title_sort |
Effect of passive ultrasonic irrigation on Enterococcus faecalis from root canals: An ex vivo study |
author |
Guerreiro-Tanomaru, Juliane Maria [UNESP] |
author_facet |
Guerreiro-Tanomaru, Juliane Maria [UNESP] Chávez-Andrade, Gisselle Moraima [UNESP] de Faria-Júnior, Norberto Batista [UNESP] Watanabe, Evandro Tanomaru-Filho, Mário [UNESP] |
author_role |
author |
author2 |
Chávez-Andrade, Gisselle Moraima [UNESP] de Faria-Júnior, Norberto Batista [UNESP] Watanabe, Evandro Tanomaru-Filho, Mário [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Guerreiro-Tanomaru, Juliane Maria [UNESP] Chávez-Andrade, Gisselle Moraima [UNESP] de Faria-Júnior, Norberto Batista [UNESP] Watanabe, Evandro Tanomaru-Filho, Mário [UNESP] |
dc.subject.por.fl_str_mv |
Endodontics Enterococcus faecalis Passive ultrasonic irrigation Root canal disinfection |
topic |
Endodontics Enterococcus faecalis Passive ultrasonic irrigation Root canal disinfection |
description |
Endodontic irrigation aims to clean and disinfect the root canal system. Passive ultrasonic irrigation (PUI) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. The aim of this study was to evaluate, ex vivo, the effectiveness of PUI in eliminating Enterococcus faecalis from root canals. Seventy-five extracted human single-root teeth were used. After root canal preparation, specimens were inoculated with E. faecalis and incubated at 37 °C for 21 days. Specimens were distributed into five groups (n=15), according to the irrigation method: PUI + saline solution (PUI/SS); PUI + 1% NaOCl (PUI/NaOCl); conventional needle irrigation (CNI) + saline solution (CNI/SS); CNI + 1% NaOCl (CNI/NaOCl); No irrigation (control). Microbiological samples were collected at three time points: initial (21 days after inoculation), post-irrigation (immediately after irrigation), and final (7 days after irrigation). Data were obtained in CFU mL-1 and subjected to analysis by ANOVA and Tukey’s tests at 5% significance level. The post-irrigation samples did not demonstrate statistical difference between PUI/SS and CNI/SS nor between PUI/NaOCl and CNI/NaOCl (p>0.05), but PUI/NaOCl and CNI/NaOCl had lower CFU mL-1 number than the other groups (p>0.05). Statistically significant difference was observed between the initial and post-irrigation samples and between the post-irrigation and final samples (p<0.05) in all groups, except in the control. The final samples of all groups presented bacterial counts similar to the initial samples. PUI or CNI with 1% NaOCl contribute to disinfection, but are unable to eradicate E. faecalis from the root canal system. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T16:58:09Z 2018-12-11T16:58:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201300022 Brazilian Dental Journal, v. 26, n. 4, p. 342-346, 2015. 1806-4760 0103-6440 http://hdl.handle.net/11449/172016 10.1590/0103-6440201300022 S0103-64402015000400342 2-s2.0-84940032448 S0103-64402015000400342.pdf |
url |
http://dx.doi.org/10.1590/0103-6440201300022 http://hdl.handle.net/11449/172016 |
identifier_str_mv |
Brazilian Dental Journal, v. 26, n. 4, p. 342-346, 2015. 1806-4760 0103-6440 10.1590/0103-6440201300022 S0103-64402015000400342 2-s2.0-84940032448 S0103-64402015000400342.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
342-346 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965510822002688 |